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The Volume of Stationary Phase is the amount of motionless part of the chromatography column. Check FAQs
Vs=k'Vmobile phaseK
Vs - Volume of Stationary Phase?k' - Capacity Factor?Vmobile phase - Volume of Mobile Phase?K - Partition Coefficient?

Volume of Stationary Phase given Capacity Factor and Partition Coefficient Example

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Here is how the Volume of Stationary Phase given Capacity Factor and Partition Coefficient equation looks like with Values.

Here is how the Volume of Stationary Phase given Capacity Factor and Partition Coefficient equation looks like with Units.

Here is how the Volume of Stationary Phase given Capacity Factor and Partition Coefficient equation looks like.

0.375Edit=3Edit5Edit40Edit
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Volume of Stationary Phase given Capacity Factor and Partition Coefficient Solution

Follow our step by step solution on how to calculate Volume of Stationary Phase given Capacity Factor and Partition Coefficient?

FIRST Step Consider the formula
Vs=k'Vmobile phaseK
Next Step Substitute values of Variables
Vs=35L40
Next Step Convert Units
Vs=30.00540
Next Step Prepare to Evaluate
Vs=30.00540
Next Step Evaluate
Vs=0.000375
LAST Step Convert to Output's Unit
Vs=0.375L

Volume of Stationary Phase given Capacity Factor and Partition Coefficient Formula Elements

Variables
Volume of Stationary Phase
The Volume of Stationary Phase is the amount of motionless part of the chromatography column.
Symbol: Vs
Measurement: VolumeUnit: L
Note: Value can be positive or negative.
Capacity Factor
The Capacity factor is directly proportional to the retention factor. The longer a component is retained by the column, the greater is the capacity factor.
Symbol: k'
Measurement: NAUnit: Unitless
Note: Value can be positive or negative.
Volume of Mobile Phase
The Volume of Mobile Phase is the amount of the solvent that runs through the chromatography column.
Symbol: Vmobile phase
Measurement: VolumeUnit: L
Note: Value can be positive or negative.
Partition Coefficient
The Partition Coefficient is defined as the ratio of the equilibrium concentrations of a dissolved substance in a two-phase system consisting of two largely immiscible solvents.
Symbol: K
Measurement: NAUnit: Unitless
Note: Value can be positive or negative.

Other Formulas to find Volume of Stationary Phase

​Go Volume of Stationary Phase given Capacity Factor
Vs=Vmobile phaseCmk'Cs

Other formulas in Volume and Concentration of Mobile and Stationary Phase category

​Go Volume of Mobile Phase given Capacity Factor
Vmobile phase=VsCsk'Cm
​Go Volume of Mobile Phase given Capacity Factor and Partition Coefficient
Vmobile phase=K(Vsk')
​Go Concentration of Mobile Phase given Capacity Factor
Cm=VsCsVmobile phasek'
​Go Concentration of Stationary Phase given Capacity Factor
Cs=CmVmobile phasek'Vs

How to Evaluate Volume of Stationary Phase given Capacity Factor and Partition Coefficient?

Volume of Stationary Phase given Capacity Factor and Partition Coefficient evaluator uses Volume of Stationary Phase = (Capacity Factor*Volume of Mobile Phase)/Partition Coefficient to evaluate the Volume of Stationary Phase, The Volume of stationary phase given capacity factor and partition coefficient formula is defined as the ratio of the volume of mobile phase and capacity factor to the partition coefficient of the solutes. Volume of Stationary Phase is denoted by Vs symbol.

How to evaluate Volume of Stationary Phase given Capacity Factor and Partition Coefficient using this online evaluator? To use this online evaluator for Volume of Stationary Phase given Capacity Factor and Partition Coefficient, enter Capacity Factor (k'), Volume of Mobile Phase (Vmobile phase) & Partition Coefficient (K) and hit the calculate button.

FAQs on Volume of Stationary Phase given Capacity Factor and Partition Coefficient

What is the formula to find Volume of Stationary Phase given Capacity Factor and Partition Coefficient?
The formula of Volume of Stationary Phase given Capacity Factor and Partition Coefficient is expressed as Volume of Stationary Phase = (Capacity Factor*Volume of Mobile Phase)/Partition Coefficient. Here is an example- 375 = (3*0.005)/40.
How to calculate Volume of Stationary Phase given Capacity Factor and Partition Coefficient?
With Capacity Factor (k'), Volume of Mobile Phase (Vmobile phase) & Partition Coefficient (K) we can find Volume of Stationary Phase given Capacity Factor and Partition Coefficient using the formula - Volume of Stationary Phase = (Capacity Factor*Volume of Mobile Phase)/Partition Coefficient.
What are the other ways to Calculate Volume of Stationary Phase?
Here are the different ways to Calculate Volume of Stationary Phase-
  • Volume of Stationary Phase=(Volume of Mobile Phase*Concentration of Mobile Phase*Capacity Factor)/Concentration of Stationary PhaseOpenImg
Can the Volume of Stationary Phase given Capacity Factor and Partition Coefficient be negative?
Yes, the Volume of Stationary Phase given Capacity Factor and Partition Coefficient, measured in Volume can be negative.
Which unit is used to measure Volume of Stationary Phase given Capacity Factor and Partition Coefficient?
Volume of Stationary Phase given Capacity Factor and Partition Coefficient is usually measured using the Liter[L] for Volume. Cubic Meter[L], Cubic Centimeter[L], Cubic Millimeter[L] are the few other units in which Volume of Stationary Phase given Capacity Factor and Partition Coefficient can be measured.
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