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Total Volume of Mobile Phase within Column given Retention Factor Formula

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Void Volume is the volume of mobile phase in a column. Check FAQs

V 0 = \frac{V R}{R f + 1}

V₀ - Void Volume?V_R - Retention Volume?R_f - Retention Factor?

Total Volume of Mobile Phase within Column given Retention Factor Example

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Here is how the Total Volume of Mobile Phase within Column given Retention Factor equation looks like with Values.

Here is how the Total Volume of Mobile Phase within Column given Retention Factor equation looks like with Units.

Here is how the Total Volume of Mobile Phase within Column given Retention Factor equation looks like.

1018.1818 = \frac{11.2}{10 + 1}

1018.1818mL = \frac{11.2L}{10 + 1}

1018.1818 = \frac{11.2}{10 + 1}

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Total Volume of Mobile Phase within Column given Retention Factor Solution

Follow our step by step solution on how to calculate Total Volume of Mobile Phase within Column given Retention Factor?

FIRST Step Consider the formula

V 0 = \frac{V R}{R f + 1}

Next Step Substitute values of Variables

∴

V 0 = \frac{11.2L}{10 + 1}

Next Step Convert Units

∴

V 0 = \frac{0.0112m³}{10 + 1}

Next Step Prepare to Evaluate

∴

V 0 = \frac{0.0112}{10 + 1}

Next Step Evaluate

∴

V 0 = 0.00101818181818182m³

Next Step Convert to Output's Unit

∴

V 0 = 1018.18181818182mL

LAST Step Rounding Answer

∴

V 0 = 1018.1818mL

Total Volume of Mobile Phase within Column given Retention Factor Formula Elements

Variables

Void Volume

Void Volume is the volume of mobile phase in a column.

Symbol: V₀

Measurement: VolumeUnit: mL

Note: Value should be greater than 0.

Formulas to find Void Volume

Retention Volume

The Retention Volume is defined as the volume of the mobile phase required to elute the solute from the column.

Symbol: V_R

Measurement: VolumeUnit: L

Note: Value can be positive or negative.

Formulas to find Retention Volume

Retention Factor

Retention Factor is defined as the ratio of the distance traveled by the center of a spot to the distance traveled by the solvent front.

Symbol: R_f

Measurement: NAUnit: Unitless

Note: Value should be greater than 0.

Formulas to find Retention Factor

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How to Evaluate Total Volume of Mobile Phase within Column given Retention Factor?

Total Volume of Mobile Phase within Column given Retention Factor evaluator uses Void Volume = Retention Volume/(Retention Factor+1) to evaluate the Void Volume, The Total Volume of Mobile Phase within Column given Retention Factor formula in column chromatography is defined as the volume of the mobile phase is the total bed volume of the column minus the volume occupied by the support particles. Void Volume is denoted by V₀ symbol.

How to evaluate Total Volume of Mobile Phase within Column given Retention Factor using this online evaluator? To use this online evaluator for Total Volume of Mobile Phase within Column given Retention Factor, enter Retention Volume (V_R) & Retention Factor (R_f) and hit the calculate button.

FAQs on Total Volume of Mobile Phase within Column given Retention Factor

What is the formula to find Total Volume of Mobile Phase within Column given Retention Factor?

The formula of Total Volume of Mobile Phase within Column given Retention Factor is expressed as Void Volume = Retention Volume/(Retention Factor+1). Here is an example- 1E+9 = 0.0112/(10+1).

How to calculate Total Volume of Mobile Phase within Column given Retention Factor?

With Retention Volume (V_R) & Retention Factor (R_f) we can find Total Volume of Mobile Phase within Column given Retention Factor using the formula - Void Volume = Retention Volume/(Retention Factor+1).

Can the Total Volume of Mobile Phase within Column given Retention Factor be negative?

No, the Total Volume of Mobile Phase within Column given Retention Factor, measured in Volume cannot be negative.

Which unit is used to measure Total Volume of Mobile Phase within Column given Retention Factor?

Total Volume of Mobile Phase within Column given Retention Factor is usually measured using the Milliliter[mL] for Volume. Cubic Meter[mL], Cubic Centimeter[mL], Cubic Millimeter[mL] are the few other units in which Total Volume of Mobile Phase within Column given Retention Factor can be measured.

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