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Substrate Concentration of Competitive Inhibition of Enzyme Catalysis Formula

GoSubstrate Concentration in Competitive Inhibition given Maximum Rate of System Formula

GoSubstrate Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration Formula

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The Substrate Concentration is the number of moles of substrate per liter solution. Check FAQs

S = \frac{V 0 \cdot (K M \cdot (1 + (\frac{I}{K i})))}{(k 2 \cdot [E 0]) - V 0}

S - Substrate Concentration?V₀ - Initial Reaction Rate?K_M - Michaelis Constant?I - Inhibitor Concentration?K_i - Enzyme Inhibitor Dissociation Constant?k₂ - Final Rate Constant?[E₀] - Initial Enzyme Concentration?

Substrate Concentration of Competitive Inhibition of Enzyme Catalysis Example

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Here is how the Substrate Concentration of Competitive Inhibition of Enzyme Catalysis equation looks like with Values.

Here is how the Substrate Concentration of Competitive Inhibition of Enzyme Catalysis equation looks like with Units.

Here is how the Substrate Concentration of Competitive Inhibition of Enzyme Catalysis equation looks like.

0.0009 = \frac{0.45 \cdot (3 \cdot (1 + (\frac{9}{19})))}{(23 \cdot 100) - 0.45}

0.0009mol/L = \frac{0.45mol/L*s \cdot (3mol/L \cdot (1 + (\frac{9mol/L}{19mol/L})))}{(23s⁻¹ \cdot 100mol/L) - 0.45mol/L*s}

0.0009 = \frac{0.45 \cdot (3 \cdot (1 + (\frac{9}{19})))}{(23 \cdot 100) - 0.45}

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Substrate Concentration of Competitive Inhibition of Enzyme Catalysis Solution

Follow our step by step solution on how to calculate Substrate Concentration of Competitive Inhibition of Enzyme Catalysis?

FIRST Step Consider the formula

S = \frac{V 0 \cdot (K M \cdot (1 + (\frac{I}{K i})))}{(k 2 \cdot [E 0]) - V 0}

Next Step Substitute values of Variables

∴

S = \frac{0.45mol/L*s \cdot (3mol/L \cdot (1 + (\frac{9mol/L}{19mol/L})))}{(23s⁻¹ \cdot 100mol/L) - 0.45mol/L*s}

Next Step Convert Units

∴

S = \frac{450mol/m³*s \cdot (3000mol/m³ \cdot (1 + (\frac{9000mol/m³}{19000mol/m³})))}{(23s⁻¹ \cdot 100000mol/m³) - 450mol/m³*s}

Next Step Prepare to Evaluate

∴

S = \frac{450 \cdot (3000 \cdot (1 + (\frac{9000}{19000})))}{(23 \cdot 100000) - 450}

Next Step Evaluate

∴

S = 0.8651578283623mol/m³

Next Step Convert to Output's Unit

∴

S = 0.0008651578283623mol/L

LAST Step Rounding Answer

∴

S = 0.0009mol/L

Substrate Concentration of Competitive Inhibition of Enzyme Catalysis Formula Elements

Variables

Substrate Concentration

The Substrate Concentration is the number of moles of substrate per liter solution.

Symbol: S

Measurement: Molar ConcentrationUnit: mol/L

Note: Value can be positive or negative.

Formulas to find Substrate Concentration

Initial Reaction Rate

The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place.

Symbol: V₀

Measurement: Reaction RateUnit: mol/L*s

Note: Value can be positive or negative.

Formulas to find Initial Reaction Rate

Michaelis Constant

The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.

Symbol: K_M

Measurement: Molar ConcentrationUnit: mol/L

Note: Value can be positive or negative.

Formulas to find Michaelis Constant

Inhibitor Concentration

The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system.

Symbol: I

Measurement: Molar ConcentrationUnit: mol/L

Note: Value can be positive or negative.

Formulas to find Inhibitor Concentration

Enzyme Inhibitor Dissociation Constant

The Enzyme Inhibitor Dissociation Constant is measured by the method in which the inhibitor is titrated into a solution of enzyme and the heat released or absorbed is measured.

Symbol: K_i

Measurement: Molar ConcentrationUnit: mol/L

Note: Value can be positive or negative.

Formulas to find Enzyme Inhibitor Dissociation Constant

Final Rate Constant

The Final Rate Constant is the rate constant when the enzyme-substrate complex on reaction with inhibitor is converted into the enzyme catalyst and product.

Symbol: k₂

Measurement: First Order Reaction Rate ConstantUnit: s⁻¹

Note: Value can be positive or negative.

Formulas to find Final Rate Constant

Initial Enzyme Concentration

The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction.

Symbol: [E₀]

Measurement: Molar ConcentrationUnit: mol/L

Note: Value should be greater than 0.

Formulas to find Initial Enzyme Concentration

Other Formulas to find Substrate Concentration

Go Substrate Concentration in Competitive Inhibition given Maximum Rate of System

S = \frac{V 0 \cdot (K M \cdot (1 + (\frac{I}{K i})))}{V max - V 0}

Go Substrate Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration

S = \frac{ES \cdot (K M \cdot (1 + (\frac{I}{K i})))}{([E 0]) - ES}

Other formulas in Competitive Inhibitor category

Go Apparent Value of Michaelis Menten Constant in Presence of Competitive Inhibition

Km app = \frac{S \cdot (V max - V 0)}{V 0}

Go Dissociation Constant for Competitive Inhibition of Enzyme Catalysis

K i = \frac{I}{(\frac{(\frac{k 2 \cdot [E 0] \cdot S}{V 0}) - S}{K M}) - 1}

How to Evaluate Substrate Concentration of Competitive Inhibition of Enzyme Catalysis?

Substrate Concentration of Competitive Inhibition of Enzyme Catalysis evaluator uses Substrate Concentration = (Initial Reaction Rate*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))))/((Final Rate Constant*Initial Enzyme Concentration)-Initial Reaction Rate) to evaluate the Substrate Concentration, The Substrate concentration of competitive inhibition of enzyme catalysis formula is defined as a plot of the reaction velocity (V0) associated with the concentration [S] of the substrate can then be used to determine values such as Vmax, initial velocity, and Km. Substrate Concentration is denoted by S symbol.

How to evaluate Substrate Concentration of Competitive Inhibition of Enzyme Catalysis using this online evaluator? To use this online evaluator for Substrate Concentration of Competitive Inhibition of Enzyme Catalysis, enter Initial Reaction Rate (V₀), Michaelis Constant (K_M), Inhibitor Concentration (I), Enzyme Inhibitor Dissociation Constant (K_i), Final Rate Constant (k₂) & Initial Enzyme Concentration ([E₀]) and hit the calculate button.

FAQs on Substrate Concentration of Competitive Inhibition of Enzyme Catalysis

What is the formula to find Substrate Concentration of Competitive Inhibition of Enzyme Catalysis?

The formula of Substrate Concentration of Competitive Inhibition of Enzyme Catalysis is expressed as Substrate Concentration = (Initial Reaction Rate*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))))/((Final Rate Constant*Initial Enzyme Concentration)-Initial Reaction Rate). Here is an example- 8.7E-7 = (450*(3000*(1+(9000/19000))))/((23*100000)-450).

How to calculate Substrate Concentration of Competitive Inhibition of Enzyme Catalysis?

With Initial Reaction Rate (V₀), Michaelis Constant (K_M), Inhibitor Concentration (I), Enzyme Inhibitor Dissociation Constant (K_i), Final Rate Constant (k₂) & Initial Enzyme Concentration ([E₀]) we can find Substrate Concentration of Competitive Inhibition of Enzyme Catalysis using the formula - Substrate Concentration = (Initial Reaction Rate*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))))/((Final Rate Constant*Initial Enzyme Concentration)-Initial Reaction Rate).

What are the other ways to Calculate Substrate Concentration?

Here are the different ways to Calculate Substrate Concentration-

Substrate Concentration=(Initial Reaction Rate*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))))/(Maximum Rate-Initial Reaction Rate)
Substrate Concentration=(Enzyme Substrate Complex Concentration*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))))/((Initial Enzyme Concentration)-Enzyme Substrate Complex Concentration)
Substrate Concentration=(Apparent Michaelis Constant*Initial Reaction Rate)/(Maximum Rate-Initial Reaction Rate)

Can the Substrate Concentration of Competitive Inhibition of Enzyme Catalysis be negative?

Yes, the Substrate Concentration of Competitive Inhibition of Enzyme Catalysis, measured in Molar Concentration can be negative.

Which unit is used to measure Substrate Concentration of Competitive Inhibition of Enzyme Catalysis?

Substrate Concentration of Competitive Inhibition of Enzyme Catalysis is usually measured using the Mole per Liter[mol/L] for Molar Concentration. Mole per Cubic Meter[mol/L], Mole per Cubic Millimeter[mol/L], Kilomole per Cubic Meter[mol/L] are the few other units in which Substrate Concentration of Competitive Inhibition of Enzyme Catalysis can be measured.

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Substrate Concentration of Competitive Inhibition of Enzyme Catalysis Formula

​GoSubstrate Concentration in Competitive Inhibition given Maximum Rate of System Formula

​GoSubstrate Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration Formula

Substrate Concentration of Competitive Inhibition of Enzyme Catalysis Example

Substrate Concentration of Competitive Inhibition of Enzyme Catalysis Solution

Substrate Concentration of Competitive Inhibition of Enzyme Catalysis Formula Elements

Other Formulas to find Substrate Concentration

Other formulas in Competitive Inhibitor category

How to Evaluate Substrate Concentration of Competitive Inhibition of Enzyme Catalysis?

FAQs on Substrate Concentration of Competitive Inhibition of Enzyme Catalysis

Variable Name

GoSubstrate Concentration in Competitive Inhibition given Maximum Rate of System Formula

GoSubstrate Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration Formula