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Substrate Concentration in Enzymatic Reaction Mechanism Formula

GoSubstrate Concentration if Michaelis Constant is very Large than Substrate Concentration Formula

GoSubstrate Concentration given Maximum Rate at Low Concentration Formula

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The Substrate Concentration is the number of moles of substrate per liter solution. Check FAQs

S = \frac{k r \cdot ES}{k f \cdot ([E 0] - ES)}

S - Substrate Concentration?k_r - Reverse Rate Constant?ES - Enzyme Substrate Complex Concentration?k_f - Forward Rate Constant?[E₀] - Initial Enzyme Concentration?

Substrate Concentration in Enzymatic Reaction Mechanism Example

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Here is how the Substrate Concentration in Enzymatic Reaction Mechanism equation looks like with Values.

Here is how the Substrate Concentration in Enzymatic Reaction Mechanism equation looks like with Units.

Here is how the Substrate Concentration in Enzymatic Reaction Mechanism equation looks like.

0.3221 = \frac{20 \cdot 10}{6.9 \cdot (100 - 10)}

0.3221mol/L = \frac{20mol/L*s \cdot 10mol/L}{6.9s⁻¹ \cdot (100mol/L - 10mol/L)}

0.3221 = \frac{20 \cdot 10}{6.9 \cdot (100 - 10)}

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Enzyme Kinetics » fx Substrate Concentration in Enzymatic Reaction Mechanism

Substrate Concentration in Enzymatic Reaction Mechanism Solution

Follow our step by step solution on how to calculate Substrate Concentration in Enzymatic Reaction Mechanism?

FIRST Step Consider the formula

S = \frac{k r \cdot ES}{k f \cdot ([E 0] - ES)}

Next Step Substitute values of Variables

∴

S = \frac{20mol/L*s \cdot 10mol/L}{6.9s⁻¹ \cdot (100mol/L - 10mol/L)}

Next Step Convert Units

∴

S = \frac{20000mol/m³*s \cdot 10000mol/m³}{6.9s⁻¹ \cdot (100000mol/m³ - 10000mol/m³)}

Next Step Prepare to Evaluate

∴

S = \frac{20000 \cdot 10000}{6.9 \cdot (100000 - 10000)}

Next Step Evaluate

∴

S = 322.061191626409mol/m³

Next Step Convert to Output's Unit

∴

S = 0.322061191626409mol/L

LAST Step Rounding Answer

∴

S = 0.3221mol/L

Substrate Concentration in Enzymatic Reaction Mechanism Formula Elements

Variables

Substrate Concentration

The Substrate Concentration is the number of moles of substrate per liter solution.

Symbol: S

Measurement: Molar ConcentrationUnit: mol/L

Note: Value can be positive or negative.

Formulas to find Substrate Concentration

Reverse Rate Constant

The Reverse Rate Constant is defined as the rate constant for the backward reaction.

Symbol: k_r

Measurement: Reaction RateUnit: mol/L*s

Note: Value can be positive or negative.

Formulas to find Reverse Rate Constant

Enzyme Substrate Complex Concentration

The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate.

Symbol: ES

Measurement: Molar ConcentrationUnit: mol/L

Note: Value can be positive or negative.

Formulas to find Enzyme Substrate Complex Concentration

Forward Rate Constant

The Forward Rate Constant is defined as the rate constant for the forward occurring reaction.

Symbol: k_f

Measurement: First Order Reaction Rate ConstantUnit: s⁻¹

Note: Value can be positive or negative.

Formulas to find Forward Rate Constant

Initial Enzyme Concentration

The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction.

Symbol: [E₀]

Measurement: Molar ConcentrationUnit: mol/L

Note: Value should be greater than 0.

Formulas to find Initial Enzyme Concentration

Other Formulas to find Substrate Concentration

Go Substrate Concentration if Michaelis Constant is very Large than Substrate Concentration

S = \frac{V 0 \cdot K M}{k cat \cdot [E 0]}

Go Substrate Concentration given Maximum Rate at Low Concentration

S = \frac{V 0 \cdot K M}{V max}

Go Substrate Concentration given Dissociation Rate Constant

S = \frac{K D \cdot ES}{[E 0] - ES}

Go Substrate Concentration given Maximum Rate and Dissociation Rate Constant

S = \frac{K D \cdot V 0}{V max - V 0}

Other formulas in Complex Concentration category

Go Substrate Concentration given Catalytic Rate Constant and Initial Enzyme Concentration

S o = \frac{K M \cdot V 0}{(k cat \cdot [E 0]) - V 0}

Go Initial Enzyme Concentration at Low Substrate Concentration

[E 0] = \frac{V 0 \cdot K M}{k cat \cdot S}

Go Enzyme Substrate Complex Concentration in Instantaneous Chemical Equilibrium

ES = \frac{k f \cdot [E 0] \cdot S}{k r + (k f \cdot S)}

Go Initial Enzyme Concentration in Enzymatic Reaction Mechanism

[E 0] = (\frac{k r \cdot ES}{k f \cdot S}) + ES

How to Evaluate Substrate Concentration in Enzymatic Reaction Mechanism?

Substrate Concentration in Enzymatic Reaction Mechanism evaluator uses Substrate Concentration = (Reverse Rate Constant*Enzyme Substrate Complex Concentration)/(Forward Rate Constant*(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration)) to evaluate the Substrate Concentration, The Substrate concentration in enzymatic reaction mechanism formula is defined as the relation with the rate constant of forward and backward reaction and with the concentration of enzyme and enzyme-substrate complex. Substrate Concentration is denoted by S symbol.

How to evaluate Substrate Concentration in Enzymatic Reaction Mechanism using this online evaluator? To use this online evaluator for Substrate Concentration in Enzymatic Reaction Mechanism, enter Reverse Rate Constant (k_r), Enzyme Substrate Complex Concentration (ES), Forward Rate Constant (k_f) & Initial Enzyme Concentration ([E₀]) and hit the calculate button.

FAQs on Substrate Concentration in Enzymatic Reaction Mechanism

What is the formula to find Substrate Concentration in Enzymatic Reaction Mechanism?

The formula of Substrate Concentration in Enzymatic Reaction Mechanism is expressed as Substrate Concentration = (Reverse Rate Constant*Enzyme Substrate Complex Concentration)/(Forward Rate Constant*(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration)). Here is an example- 0.000322 = (20000*10000)/(6.9*(100000-10000)).

How to calculate Substrate Concentration in Enzymatic Reaction Mechanism?

With Reverse Rate Constant (k_r), Enzyme Substrate Complex Concentration (ES), Forward Rate Constant (k_f) & Initial Enzyme Concentration ([E₀]) we can find Substrate Concentration in Enzymatic Reaction Mechanism using the formula - Substrate Concentration = (Reverse Rate Constant*Enzyme Substrate Complex Concentration)/(Forward Rate Constant*(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration)).

What are the other ways to Calculate Substrate Concentration?

Here are the different ways to Calculate Substrate Concentration-

Substrate Concentration=(Initial Reaction Rate*Michaelis Constant)/(Catalytic Rate Constant*Initial Enzyme Concentration)
Substrate Concentration=(Initial Reaction Rate*Michaelis Constant)/Maximum Rate
Substrate Concentration=(Dissociation Rate Constant*Enzyme Substrate Complex Concentration)/(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration)

Can the Substrate Concentration in Enzymatic Reaction Mechanism be negative?

Yes, the Substrate Concentration in Enzymatic Reaction Mechanism, measured in Molar Concentration can be negative.

Which unit is used to measure Substrate Concentration in Enzymatic Reaction Mechanism?

Substrate Concentration in Enzymatic Reaction Mechanism is usually measured using the Mole per Liter[mol/L] for Molar Concentration. Mole per Cubic Meter[mol/L], Mole per Cubic Millimeter[mol/L], Kilomole per Cubic Meter[mol/L] are the few other units in which Substrate Concentration in Enzymatic Reaction Mechanism can be measured.

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Substrate Concentration in Enzymatic Reaction Mechanism Formula

​GoSubstrate Concentration if Michaelis Constant is very Large than Substrate Concentration Formula

​GoSubstrate Concentration given Maximum Rate at Low Concentration Formula

Substrate Concentration in Enzymatic Reaction Mechanism Example

Substrate Concentration in Enzymatic Reaction Mechanism Solution

Substrate Concentration in Enzymatic Reaction Mechanism Formula Elements

Other Formulas to find Substrate Concentration

Other formulas in Complex Concentration category

How to Evaluate Substrate Concentration in Enzymatic Reaction Mechanism?

FAQs on Substrate Concentration in Enzymatic Reaction Mechanism

Variable Name

GoSubstrate Concentration if Michaelis Constant is very Large than Substrate Concentration Formula

GoSubstrate Concentration given Maximum Rate at Low Concentration Formula