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Substrate Concentration if Michaelis Constant is very Large than Substrate Concentration Formula

GoSubstrate Concentration given Maximum Rate at Low Concentration Formula

GoSubstrate Concentration in Enzymatic Reaction Mechanism Formula

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The Substrate Concentration is the number of moles of substrate per liter solution. Check FAQs

S = \frac{V 0 \cdot K M}{k cat \cdot [E 0]}

S - Substrate Concentration?V₀ - Initial Reaction Rate?K_M - Michaelis Constant?k_cat - Catalytic Rate Constant?[E₀] - Initial Enzyme Concentration?

Substrate Concentration if Michaelis Constant is very Large than Substrate Concentration Example

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Here is how the Substrate Concentration if Michaelis Constant is very Large than Substrate Concentration equation looks like with Values.

Here is how the Substrate Concentration if Michaelis Constant is very Large than Substrate Concentration equation looks like with Units.

Here is how the Substrate Concentration if Michaelis Constant is very Large than Substrate Concentration equation looks like.

0.0208 = \frac{0.45 \cdot 3}{0.65 \cdot 100}

0.0208mol/L = \frac{0.45mol/L*s \cdot 3mol/L}{0.65s⁻¹ \cdot 100mol/L}

0.0208 = \frac{0.45 \cdot 3}{0.65 \cdot 100}

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Enzyme Kinetics » fx Substrate Concentration if Michaelis Constant is very Large than Substrate Concentration

Substrate Concentration if Michaelis Constant is very Large than Substrate Concentration Solution

Follow our step by step solution on how to calculate Substrate Concentration if Michaelis Constant is very Large than Substrate Concentration?

FIRST Step Consider the formula

S = \frac{V 0 \cdot K M}{k cat \cdot [E 0]}

Next Step Substitute values of Variables

∴

S = \frac{0.45mol/L*s \cdot 3mol/L}{0.65s⁻¹ \cdot 100mol/L}

Next Step Convert Units

∴

S = \frac{450mol/m³*s \cdot 3000mol/m³}{0.65s⁻¹ \cdot 100000mol/m³}

Next Step Prepare to Evaluate

∴

S = \frac{450 \cdot 3000}{0.65 \cdot 100000}

Next Step Evaluate

∴

S = 20.7692307692308mol/m³

Next Step Convert to Output's Unit

∴

S = 0.0207692307692308mol/L

LAST Step Rounding Answer

∴

S = 0.0208mol/L

Substrate Concentration if Michaelis Constant is very Large than Substrate Concentration Formula Elements

Variables

Substrate Concentration

The Substrate Concentration is the number of moles of substrate per liter solution.

Symbol: S

Measurement: Molar ConcentrationUnit: mol/L

Note: Value can be positive or negative.

Formulas to find Substrate Concentration

Initial Reaction Rate

The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place.

Symbol: V₀

Measurement: Reaction RateUnit: mol/L*s

Note: Value can be positive or negative.

Formulas to find Initial Reaction Rate

Michaelis Constant

The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.

Symbol: K_M

Measurement: Molar ConcentrationUnit: mol/L

Note: Value can be positive or negative.

Formulas to find Michaelis Constant

Catalytic Rate Constant

The Catalytic Rate Constant is defined as the rate constant for conversion of the enzyme-substrate complex to enzyme and product.

Symbol: k_cat

Measurement: First Order Reaction Rate ConstantUnit: s⁻¹

Note: Value can be positive or negative.

Formulas to find Catalytic Rate Constant

Initial Enzyme Concentration

The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction.

Symbol: [E₀]

Measurement: Molar ConcentrationUnit: mol/L

Note: Value should be greater than 0.

Formulas to find Initial Enzyme Concentration

Other Formulas to find Substrate Concentration

Go Substrate Concentration given Maximum Rate at Low Concentration

S = \frac{V 0 \cdot K M}{V max}

Go Substrate Concentration in Enzymatic Reaction Mechanism

S = \frac{k r \cdot ES}{k f \cdot ([E 0] - ES)}

Go Substrate Concentration given Dissociation Rate Constant

S = \frac{K D \cdot ES}{[E 0] - ES}

Go Substrate Concentration given Maximum Rate and Dissociation Rate Constant

S = \frac{K D \cdot V 0}{V max - V 0}

Other formulas in Complex Concentration category

Go Substrate Concentration given Catalytic Rate Constant and Initial Enzyme Concentration

S o = \frac{K M \cdot V 0}{(k cat \cdot [E 0]) - V 0}

Go Initial Enzyme Concentration at Low Substrate Concentration

[E 0] = \frac{V 0 \cdot K M}{k cat \cdot S}

Go Enzyme Substrate Complex Concentration in Instantaneous Chemical Equilibrium

ES = \frac{k f \cdot [E 0] \cdot S}{k r + (k f \cdot S)}

Go Initial Enzyme Concentration in Enzymatic Reaction Mechanism

[E 0] = (\frac{k r \cdot ES}{k f \cdot S}) + ES

How to Evaluate Substrate Concentration if Michaelis Constant is very Large than Substrate Concentration?

Substrate Concentration if Michaelis Constant is very Large than Substrate Concentration evaluator uses Substrate Concentration = (Initial Reaction Rate*Michaelis Constant)/(Catalytic Rate Constant*Initial Enzyme Concentration) to evaluate the Substrate Concentration, The Substrate concentration if Michaelis constant is very large than substrate concentration formula is defined as the relation with initial reaction rate and initial enzyme concentration. Here substrate concentration is very less i.e negligible in comparison to Michaelis constant. Substrate Concentration is denoted by S symbol.

How to evaluate Substrate Concentration if Michaelis Constant is very Large than Substrate Concentration using this online evaluator? To use this online evaluator for Substrate Concentration if Michaelis Constant is very Large than Substrate Concentration, enter Initial Reaction Rate (V₀), Michaelis Constant (K_M), Catalytic Rate Constant (k_cat) & Initial Enzyme Concentration ([E₀]) and hit the calculate button.

FAQs on Substrate Concentration if Michaelis Constant is very Large than Substrate Concentration

What is the formula to find Substrate Concentration if Michaelis Constant is very Large than Substrate Concentration?

The formula of Substrate Concentration if Michaelis Constant is very Large than Substrate Concentration is expressed as Substrate Concentration = (Initial Reaction Rate*Michaelis Constant)/(Catalytic Rate Constant*Initial Enzyme Concentration). Here is an example- 2.1E-5 = (450*3000)/(0.65*100000).

How to calculate Substrate Concentration if Michaelis Constant is very Large than Substrate Concentration?

With Initial Reaction Rate (V₀), Michaelis Constant (K_M), Catalytic Rate Constant (k_cat) & Initial Enzyme Concentration ([E₀]) we can find Substrate Concentration if Michaelis Constant is very Large than Substrate Concentration using the formula - Substrate Concentration = (Initial Reaction Rate*Michaelis Constant)/(Catalytic Rate Constant*Initial Enzyme Concentration).

What are the other ways to Calculate Substrate Concentration?

Here are the different ways to Calculate Substrate Concentration-

Substrate Concentration=(Initial Reaction Rate*Michaelis Constant)/Maximum Rate
Substrate Concentration=(Reverse Rate Constant*Enzyme Substrate Complex Concentration)/(Forward Rate Constant*(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration))
Substrate Concentration=(Dissociation Rate Constant*Enzyme Substrate Complex Concentration)/(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration)

Can the Substrate Concentration if Michaelis Constant is very Large than Substrate Concentration be negative?

Yes, the Substrate Concentration if Michaelis Constant is very Large than Substrate Concentration, measured in Molar Concentration can be negative.

Which unit is used to measure Substrate Concentration if Michaelis Constant is very Large than Substrate Concentration?

Substrate Concentration if Michaelis Constant is very Large than Substrate Concentration is usually measured using the Mole per Liter[mol/L] for Molar Concentration. Mole per Cubic Meter[mol/L], Mole per Cubic Millimeter[mol/L], Kilomole per Cubic Meter[mol/L] are the few other units in which Substrate Concentration if Michaelis Constant is very Large than Substrate Concentration can be measured.

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Substrate Concentration if Michaelis Constant is very Large than Substrate Concentration Formula

​GoSubstrate Concentration given Maximum Rate at Low Concentration Formula

​GoSubstrate Concentration in Enzymatic Reaction Mechanism Formula

Substrate Concentration if Michaelis Constant is very Large than Substrate Concentration Example

Substrate Concentration if Michaelis Constant is very Large than Substrate Concentration Solution

Substrate Concentration if Michaelis Constant is very Large than Substrate Concentration Formula Elements

Other Formulas to find Substrate Concentration

Other formulas in Complex Concentration category

How to Evaluate Substrate Concentration if Michaelis Constant is very Large than Substrate Concentration?

FAQs on Substrate Concentration if Michaelis Constant is very Large than Substrate Concentration

Variable Name

GoSubstrate Concentration given Maximum Rate at Low Concentration Formula

GoSubstrate Concentration in Enzymatic Reaction Mechanism Formula