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The Substrate Concentration is the number of moles of substrate per liter solution. Check FAQs
S=V0(αα')KM((1α')Vmax)-V0
S - Substrate Concentration?V0 - Initial Reaction Rate?α - Enzyme Modifying Factor?α' - Enzyme Substrate Modifying Factor?KM - Michaelis Constant?Vmax - Maximum Rate?

Substrate Concentration given Modifying Factor in Michaelis Menten Equation Example

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With units
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Here is how the Substrate Concentration given Modifying Factor in Michaelis Menten Equation equation looks like with Values.

Here is how the Substrate Concentration given Modifying Factor in Michaelis Menten Equation equation looks like with Units.

Here is how the Substrate Concentration given Modifying Factor in Michaelis Menten Equation equation looks like.

0.1726Edit=0.45Edit(5Edit2Edit)3Edit((12Edit)40Edit)-0.45Edit
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Substrate Concentration given Modifying Factor in Michaelis Menten Equation Solution

Follow our step by step solution on how to calculate Substrate Concentration given Modifying Factor in Michaelis Menten Equation?

FIRST Step Consider the formula
S=V0(αα')KM((1α')Vmax)-V0
Next Step Substitute values of Variables
S=0.45mol/L*s(52)3mol/L((12)40mol/L*s)-0.45mol/L*s
Next Step Convert Units
S=450mol/m³*s(52)3000mol/m³((12)40000mol/m³*s)-450mol/m³*s
Next Step Prepare to Evaluate
S=450(52)3000((12)40000)-450
Next Step Evaluate
S=172.634271099744mol/m³
Next Step Convert to Output's Unit
S=0.172634271099744mol/L
LAST Step Rounding Answer
S=0.1726mol/L

Substrate Concentration given Modifying Factor in Michaelis Menten Equation Formula Elements

Variables
Substrate Concentration
The Substrate Concentration is the number of moles of substrate per liter solution.
Symbol: S
Measurement: Molar ConcentrationUnit: mol/L
Note: Value can be positive or negative.
Initial Reaction Rate
The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place.
Symbol: V0
Measurement: Reaction RateUnit: mol/L*s
Note: Value can be positive or negative.
Enzyme Modifying Factor
The Enzyme Modifying Factor is defined by the inhibitor concentration and the dissociation constants of enzyme.
Symbol: α
Measurement: NAUnit: Unitless
Note: Value can be positive or negative.
Enzyme Substrate Modifying Factor
The Enzyme Substrate Modifying Factor is defined by the inhibitor concentration and the dissociation constant of the enzyme-substrate complex.
Symbol: α'
Measurement: NAUnit: Unitless
Note: Value can be positive or negative.
Michaelis Constant
The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.
Symbol: KM
Measurement: Molar ConcentrationUnit: mol/L
Note: Value can be positive or negative.
Maximum Rate
The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration.
Symbol: Vmax
Measurement: Reaction RateUnit: mol/L*s
Note: Value can be positive or negative.

Other Formulas to find Substrate Concentration

​Go Substrate Concentration of Competitive Inhibition of Enzyme Catalysis
S=V0(KM(1+(IKi)))(k2[E0])-V0
​Go Substrate Concentration in Competitive Inhibition given Maximum Rate of System
S=V0(KM(1+(IKi)))Vmax-V0
​Go Substrate Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration
S=ES(KM(1+(IKi)))([E0])-ES
​Go Substrate Concentration given Apparent value of Michaelis Menten Constant
S=KmappV0Vmax-V0

Other formulas in Competitive Inhibitor category

​Go Apparent Value of Michaelis Menten Constant in Presence of Competitive Inhibition
Kmapp=S(Vmax-V0)V0
​Go Dissociation Constant for Competitive Inhibition of Enzyme Catalysis
Ki=I((k2[E0]SV0)-SKM)-1
​Go Dissociation Constant in Competitive Inhibition given Maximum Rate of System
Ki=I((VmaxSV0)-SKM)-1
​Go Dissociation Constant of Enzyme given Modifying Factor of Enzyme
Kei=Iα-1

How to Evaluate Substrate Concentration given Modifying Factor in Michaelis Menten Equation?

Substrate Concentration given Modifying Factor in Michaelis Menten Equation evaluator uses Substrate Concentration = (Initial Reaction Rate*(Enzyme Modifying Factor/Enzyme Substrate Modifying Factor)*Michaelis Constant)/(((1/Enzyme Substrate Modifying Factor)*Maximum Rate)-Initial Reaction Rate) to evaluate the Substrate Concentration, The Substrate concentration given modifying factor in Michaelis Menten equation formula is defined as the enzyme activity under various substrate and inhibitor concentrations, to obtain a modified Michaelis–Menten equation. Substrate Concentration is denoted by S symbol.

How to evaluate Substrate Concentration given Modifying Factor in Michaelis Menten Equation using this online evaluator? To use this online evaluator for Substrate Concentration given Modifying Factor in Michaelis Menten Equation, enter Initial Reaction Rate (V0), Enzyme Modifying Factor (α), Enzyme Substrate Modifying Factor '), Michaelis Constant (KM) & Maximum Rate (Vmax) and hit the calculate button.

FAQs on Substrate Concentration given Modifying Factor in Michaelis Menten Equation

What is the formula to find Substrate Concentration given Modifying Factor in Michaelis Menten Equation?
The formula of Substrate Concentration given Modifying Factor in Michaelis Menten Equation is expressed as Substrate Concentration = (Initial Reaction Rate*(Enzyme Modifying Factor/Enzyme Substrate Modifying Factor)*Michaelis Constant)/(((1/Enzyme Substrate Modifying Factor)*Maximum Rate)-Initial Reaction Rate). Here is an example- 0.000173 = (450*(5/2)*3000)/(((1/2)*40000)-450).
How to calculate Substrate Concentration given Modifying Factor in Michaelis Menten Equation?
With Initial Reaction Rate (V0), Enzyme Modifying Factor (α), Enzyme Substrate Modifying Factor '), Michaelis Constant (KM) & Maximum Rate (Vmax) we can find Substrate Concentration given Modifying Factor in Michaelis Menten Equation using the formula - Substrate Concentration = (Initial Reaction Rate*(Enzyme Modifying Factor/Enzyme Substrate Modifying Factor)*Michaelis Constant)/(((1/Enzyme Substrate Modifying Factor)*Maximum Rate)-Initial Reaction Rate).
What are the other ways to Calculate Substrate Concentration?
Here are the different ways to Calculate Substrate Concentration-
  • Substrate Concentration=(Initial Reaction Rate*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))))/((Final Rate Constant*Initial Enzyme Concentration)-Initial Reaction Rate)OpenImg
  • Substrate Concentration=(Initial Reaction Rate*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))))/(Maximum Rate-Initial Reaction Rate)OpenImg
  • Substrate Concentration=(Enzyme Substrate Complex Concentration*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))))/((Initial Enzyme Concentration)-Enzyme Substrate Complex Concentration)OpenImg
Can the Substrate Concentration given Modifying Factor in Michaelis Menten Equation be negative?
Yes, the Substrate Concentration given Modifying Factor in Michaelis Menten Equation, measured in Molar Concentration can be negative.
Which unit is used to measure Substrate Concentration given Modifying Factor in Michaelis Menten Equation?
Substrate Concentration given Modifying Factor in Michaelis Menten Equation is usually measured using the Mole per Liter[mol/L] for Molar Concentration. Mole per Cubic Meter[mol/L], Mole per Cubic Millimeter[mol/L], Kilomole per Cubic Meter[mol/L] are the few other units in which Substrate Concentration given Modifying Factor in Michaelis Menten Equation can be measured.
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