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The Substrate Concentration is the number of moles of substrate per liter solution. Check FAQs
S=KDES[E0]-ES
S - Substrate Concentration?KD - Dissociation Rate Constant?ES - Enzyme Substrate Complex Concentration?[E0] - Initial Enzyme Concentration?

Substrate Concentration given Dissociation Rate Constant Example

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With units
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Here is how the Substrate Concentration given Dissociation Rate Constant equation looks like with Values.

Here is how the Substrate Concentration given Dissociation Rate Constant equation looks like with Units.

Here is how the Substrate Concentration given Dissociation Rate Constant equation looks like.

0.6333Edit=5.7Edit10Edit100Edit-10Edit
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Substrate Concentration given Dissociation Rate Constant Solution

Follow our step by step solution on how to calculate Substrate Concentration given Dissociation Rate Constant?

FIRST Step Consider the formula
S=KDES[E0]-ES
Next Step Substitute values of Variables
S=5.7mol/L10mol/L100mol/L-10mol/L
Next Step Convert Units
S=5700mol/m³10000mol/m³100000mol/m³-10000mol/m³
Next Step Prepare to Evaluate
S=570010000100000-10000
Next Step Evaluate
S=633.333333333333mol/m³
Next Step Convert to Output's Unit
S=0.633333333333333mol/L
LAST Step Rounding Answer
S=0.6333mol/L

Substrate Concentration given Dissociation Rate Constant Formula Elements

Variables
Substrate Concentration
The Substrate Concentration is the number of moles of substrate per liter solution.
Symbol: S
Measurement: Molar ConcentrationUnit: mol/L
Note: Value can be positive or negative.
Dissociation Rate Constant
The Dissociation Rate Constant is the ratio of reverse and forward rate constant.
Symbol: KD
Measurement: Molar ConcentrationUnit: mol/L
Note: Value can be positive or negative.
Enzyme Substrate Complex Concentration
The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate.
Symbol: ES
Measurement: Molar ConcentrationUnit: mol/L
Note: Value can be positive or negative.
Initial Enzyme Concentration
The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction.
Symbol: [E0]
Measurement: Molar ConcentrationUnit: mol/L
Note: Value should be greater than 0.

Other Formulas to find Substrate Concentration

​Go Substrate Concentration if Michaelis Constant is very Large than Substrate Concentration
S=V0KMkcat[E0]
​Go Substrate Concentration given Maximum Rate at Low Concentration
S=V0KMVmax
​Go Substrate Concentration in Enzymatic Reaction Mechanism
S=krESkf([E0]-ES)
​Go Substrate Concentration given Maximum Rate and Dissociation Rate Constant
S=KDV0Vmax-V0

Other formulas in Complex Concentration category

​Go Substrate Concentration given Catalytic Rate Constant and Initial Enzyme Concentration
So=KMV0(kcat[E0])-V0
​Go Initial Enzyme Concentration at Low Substrate Concentration
[E0]=V0KMkcatS
​Go Enzyme Substrate Complex Concentration in Instantaneous Chemical Equilibrium
ES=kf[E0]Skr+(kfS)
​Go Initial Enzyme Concentration in Enzymatic Reaction Mechanism
[E0]=(krESkfS)+ES

How to Evaluate Substrate Concentration given Dissociation Rate Constant?

Substrate Concentration given Dissociation Rate Constant evaluator uses Substrate Concentration = (Dissociation Rate Constant*Enzyme Substrate Complex Concentration)/(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration) to evaluate the Substrate Concentration, The Substrate concentration given dissociation rate constant formula is defined as the relation with the dissociation rate constant and the concentration of enzyme and substrate. Substrate Concentration is denoted by S symbol.

How to evaluate Substrate Concentration given Dissociation Rate Constant using this online evaluator? To use this online evaluator for Substrate Concentration given Dissociation Rate Constant, enter Dissociation Rate Constant (KD), Enzyme Substrate Complex Concentration (ES) & Initial Enzyme Concentration ([E0]) and hit the calculate button.

FAQs on Substrate Concentration given Dissociation Rate Constant

What is the formula to find Substrate Concentration given Dissociation Rate Constant?
The formula of Substrate Concentration given Dissociation Rate Constant is expressed as Substrate Concentration = (Dissociation Rate Constant*Enzyme Substrate Complex Concentration)/(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration). Here is an example- 0.000633 = (5700*10000)/(100000-10000).
How to calculate Substrate Concentration given Dissociation Rate Constant?
With Dissociation Rate Constant (KD), Enzyme Substrate Complex Concentration (ES) & Initial Enzyme Concentration ([E0]) we can find Substrate Concentration given Dissociation Rate Constant using the formula - Substrate Concentration = (Dissociation Rate Constant*Enzyme Substrate Complex Concentration)/(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration).
What are the other ways to Calculate Substrate Concentration?
Here are the different ways to Calculate Substrate Concentration-
  • Substrate Concentration=(Initial Reaction Rate*Michaelis Constant)/(Catalytic Rate Constant*Initial Enzyme Concentration)OpenImg
  • Substrate Concentration=(Initial Reaction Rate*Michaelis Constant)/Maximum RateOpenImg
  • Substrate Concentration=(Reverse Rate Constant*Enzyme Substrate Complex Concentration)/(Forward Rate Constant*(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration))OpenImg
Can the Substrate Concentration given Dissociation Rate Constant be negative?
Yes, the Substrate Concentration given Dissociation Rate Constant, measured in Molar Concentration can be negative.
Which unit is used to measure Substrate Concentration given Dissociation Rate Constant?
Substrate Concentration given Dissociation Rate Constant is usually measured using the Mole per Liter[mol/L] for Molar Concentration. Mole per Cubic Meter[mol/L], Mole per Cubic Millimeter[mol/L], Kilomole per Cubic Meter[mol/L] are the few other units in which Substrate Concentration given Dissociation Rate Constant can be measured.
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