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Substrate Concentration given Apparent value of Michaelis Menten Constant Formula

GoSubstrate Concentration of Competitive Inhibition of Enzyme Catalysis Formula

GoSubstrate Concentration in Competitive Inhibition given Maximum Rate of System Formula

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The Substrate Concentration is the number of moles of substrate per liter solution. Check FAQs

S = \frac{Km app \cdot V 0}{V max - V 0}

S - Substrate Concentration?Km^app - Apparent Michaelis Constant?V₀ - Initial Reaction Rate?V_max - Maximum Rate?

Substrate Concentration given Apparent value of Michaelis Menten Constant Example

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Here is how the Substrate Concentration given Apparent value of Michaelis Menten Constant equation looks like with Values.

Here is how the Substrate Concentration given Apparent value of Michaelis Menten Constant equation looks like with Units.

Here is how the Substrate Concentration given Apparent value of Michaelis Menten Constant equation looks like.

0.1365 = \frac{12 \cdot 0.45}{40 - 0.45}

0.1365mol/L = \frac{12mol/L \cdot 0.45mol/L*s}{40mol/L*s - 0.45mol/L*s}

0.1365 = \frac{12 \cdot 0.45}{40 - 0.45}

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Substrate Concentration given Apparent value of Michaelis Menten Constant Solution

Follow our step by step solution on how to calculate Substrate Concentration given Apparent value of Michaelis Menten Constant?

FIRST Step Consider the formula

S = \frac{Km app \cdot V 0}{V max - V 0}

Next Step Substitute values of Variables

∴

S = \frac{12mol/L \cdot 0.45mol/L*s}{40mol/L*s - 0.45mol/L*s}

Next Step Convert Units

∴

S = \frac{12000mol/m³ \cdot 450mol/m³*s}{40000mol/m³*s - 450mol/m³*s}

Next Step Prepare to Evaluate

∴

S = \frac{12000 \cdot 450}{40000 - 450}

Next Step Evaluate

∴

S = 136.53603034134mol/m³

Next Step Convert to Output's Unit

∴

S = 0.13653603034134mol/L

LAST Step Rounding Answer

∴

S = 0.1365mol/L

Substrate Concentration given Apparent value of Michaelis Menten Constant Formula Elements

Variables

Substrate Concentration

The Substrate Concentration is the number of moles of substrate per liter solution.

Symbol: S

Measurement: Molar ConcentrationUnit: mol/L

Note: Value can be positive or negative.

Formulas to find Substrate Concentration

Apparent Michaelis Constant

The Apparent Michaelis Constant is defined as the Michaelis–Menten constant in the presence of a competitive inhibitor.

Symbol: Km^app

Measurement: Molar ConcentrationUnit: mol/L

Note: Value can be positive or negative.

Formulas to find Apparent Michaelis Constant

Initial Reaction Rate

The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place.

Symbol: V₀

Measurement: Reaction RateUnit: mol/L*s

Note: Value can be positive or negative.

Formulas to find Initial Reaction Rate

Maximum Rate

The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration.

Symbol: V_max

Measurement: Reaction RateUnit: mol/L*s

Note: Value can be positive or negative.

Formulas to find Maximum Rate

Other Formulas to find Substrate Concentration

Go Substrate Concentration of Competitive Inhibition of Enzyme Catalysis

S = \frac{V 0 \cdot (K M \cdot (1 + (\frac{I}{K i})))}{(k 2 \cdot [E 0]) - V 0}

Go Substrate Concentration in Competitive Inhibition given Maximum Rate of System

S = \frac{V 0 \cdot (K M \cdot (1 + (\frac{I}{K i})))}{V max - V 0}

Go Substrate Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration

S = \frac{ES \cdot (K M \cdot (1 + (\frac{I}{K i})))}{([E 0]) - ES}

Go Substrate Concentration given Modifying Factor in Michaelis Menten Equation

S = \frac{V 0 \cdot (\frac{α}{α'}) \cdot K M}{((\frac{1}{α'}) \cdot V max) - V 0}

Other formulas in Competitive Inhibitor category

Go Apparent Value of Michaelis Menten Constant in Presence of Competitive Inhibition

Km app = \frac{S \cdot (V max - V 0)}{V 0}

Go Dissociation Constant for Competitive Inhibition of Enzyme Catalysis

K i = \frac{I}{(\frac{(\frac{k 2 \cdot [E 0] \cdot S}{V 0}) - S}{K M}) - 1}

Go Dissociation Constant in Competitive Inhibition given Maximum Rate of System

K i = \frac{I}{(\frac{(\frac{V max \cdot S}{V 0}) - S}{K M}) - 1}

Go Dissociation Constant of Enzyme given Modifying Factor of Enzyme

K ei = \frac{I}{α - 1}

How to Evaluate Substrate Concentration given Apparent value of Michaelis Menten Constant?

Substrate Concentration given Apparent value of Michaelis Menten Constant evaluator uses Substrate Concentration = (Apparent Michaelis Constant*Initial Reaction Rate)/(Maximum Rate-Initial Reaction Rate) to evaluate the Substrate Concentration, The Substrate concentration given apparent value of Michaelis Menten constant formula is defined as a plot of the reaction velocity (V0) associated with the concentration [S] of the substrate can then be used to determine values such as Vmax, initial velocity, and Km(app). Substrate Concentration is denoted by S symbol.

How to evaluate Substrate Concentration given Apparent value of Michaelis Menten Constant using this online evaluator? To use this online evaluator for Substrate Concentration given Apparent value of Michaelis Menten Constant, enter Apparent Michaelis Constant (Km^app), Initial Reaction Rate (V₀) & Maximum Rate (V_max) and hit the calculate button.

FAQs on Substrate Concentration given Apparent value of Michaelis Menten Constant

What is the formula to find Substrate Concentration given Apparent value of Michaelis Menten Constant?

The formula of Substrate Concentration given Apparent value of Michaelis Menten Constant is expressed as Substrate Concentration = (Apparent Michaelis Constant*Initial Reaction Rate)/(Maximum Rate-Initial Reaction Rate). Here is an example- 0.000137 = (12000*450)/(40000-450).

How to calculate Substrate Concentration given Apparent value of Michaelis Menten Constant?

With Apparent Michaelis Constant (Km^app), Initial Reaction Rate (V₀) & Maximum Rate (V_max) we can find Substrate Concentration given Apparent value of Michaelis Menten Constant using the formula - Substrate Concentration = (Apparent Michaelis Constant*Initial Reaction Rate)/(Maximum Rate-Initial Reaction Rate).

What are the other ways to Calculate Substrate Concentration?

Here are the different ways to Calculate Substrate Concentration-

Substrate Concentration=(Initial Reaction Rate*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))))/((Final Rate Constant*Initial Enzyme Concentration)-Initial Reaction Rate)
Substrate Concentration=(Initial Reaction Rate*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))))/(Maximum Rate-Initial Reaction Rate)
Substrate Concentration=(Enzyme Substrate Complex Concentration*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))))/((Initial Enzyme Concentration)-Enzyme Substrate Complex Concentration)

Can the Substrate Concentration given Apparent value of Michaelis Menten Constant be negative?

Yes, the Substrate Concentration given Apparent value of Michaelis Menten Constant, measured in Molar Concentration can be negative.

Which unit is used to measure Substrate Concentration given Apparent value of Michaelis Menten Constant?

Substrate Concentration given Apparent value of Michaelis Menten Constant is usually measured using the Mole per Liter[mol/L] for Molar Concentration. Mole per Cubic Meter[mol/L], Mole per Cubic Millimeter[mol/L], Kilomole per Cubic Meter[mol/L] are the few other units in which Substrate Concentration given Apparent value of Michaelis Menten Constant can be measured.

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Substrate Concentration given Apparent value of Michaelis Menten Constant Formula

​GoSubstrate Concentration of Competitive Inhibition of Enzyme Catalysis Formula

​GoSubstrate Concentration in Competitive Inhibition given Maximum Rate of System Formula

Substrate Concentration given Apparent value of Michaelis Menten Constant Example

Substrate Concentration given Apparent value of Michaelis Menten Constant Solution

Substrate Concentration given Apparent value of Michaelis Menten Constant Formula Elements

Other Formulas to find Substrate Concentration

Other formulas in Competitive Inhibitor category

How to Evaluate Substrate Concentration given Apparent value of Michaelis Menten Constant?

FAQs on Substrate Concentration given Apparent value of Michaelis Menten Constant

Variable Name

GoSubstrate Concentration of Competitive Inhibition of Enzyme Catalysis Formula

GoSubstrate Concentration in Competitive Inhibition given Maximum Rate of System Formula