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The Reverse Rate Constant is defined as the rate constant for the backward reaction. Check FAQs
kr=kfS([E0]-ES)ES
kr - Reverse Rate Constant?kf - Forward Rate Constant?S - Substrate Concentration?[E0] - Initial Enzyme Concentration?ES - Enzyme Substrate Complex Concentration?

Reverse Rate Constant in Enzymatic Reaction Mechanism Example

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Here is how the Reverse Rate Constant in Enzymatic Reaction Mechanism equation looks like with Values.

Here is how the Reverse Rate Constant in Enzymatic Reaction Mechanism equation looks like with Units.

Here is how the Reverse Rate Constant in Enzymatic Reaction Mechanism equation looks like.

93.15Edit=6.9Edit1.5Edit(100Edit-10Edit)10Edit
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Reverse Rate Constant in Enzymatic Reaction Mechanism Solution

Follow our step by step solution on how to calculate Reverse Rate Constant in Enzymatic Reaction Mechanism?

FIRST Step Consider the formula
kr=kfS([E0]-ES)ES
Next Step Substitute values of Variables
kr=6.9s⁻¹1.5mol/L(100mol/L-10mol/L)10mol/L
Next Step Convert Units
kr=6.9s⁻¹1500mol/m³(100000mol/m³-10000mol/m³)10000mol/m³
Next Step Prepare to Evaluate
kr=6.91500(100000-10000)10000
Next Step Evaluate
kr=93150mol/m³*s
LAST Step Convert to Output's Unit
kr=93.15mol/L*s

Reverse Rate Constant in Enzymatic Reaction Mechanism Formula Elements

Variables
Reverse Rate Constant
The Reverse Rate Constant is defined as the rate constant for the backward reaction.
Symbol: kr
Measurement: Reaction RateUnit: mol/L*s
Note: Value can be positive or negative.
Forward Rate Constant
The Forward Rate Constant is defined as the rate constant for the forward occurring reaction.
Symbol: kf
Measurement: First Order Reaction Rate ConstantUnit: s⁻¹
Note: Value can be positive or negative.
Substrate Concentration
The Substrate Concentration is the number of moles of substrate per liter solution.
Symbol: S
Measurement: Molar ConcentrationUnit: mol/L
Note: Value can be positive or negative.
Initial Enzyme Concentration
The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction.
Symbol: [E0]
Measurement: Molar ConcentrationUnit: mol/L
Note: Value should be greater than 0.
Enzyme Substrate Complex Concentration
The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate.
Symbol: ES
Measurement: Molar ConcentrationUnit: mol/L
Note: Value can be positive or negative.

Other Formulas to find Reverse Rate Constant

​Go Reverse Rate Constant given Dissociation Rate Constant
kr=(KDkf)
​Go Reverse Rate Constant given Forward and Catalytic Rate Constants
kr=(kfESES)-kcat
​Go Reverse Rate Constant given Michaelis Constant
kr=(KMkf)-kcat

Other formulas in Rate Constants of Enzymatic Reaction category

​Go Catalytic Rate Constant at Low Substrate Concentration
kcat=V0KM[E0]S
​Go Forward Rate Constant in Enzymatic Reaction mechanism
kf=krESS([E0]-ES)
​Go Dissociation Rate Constant in Enzymatic Reaction Mechanism
KD=krkf
​Go Forward Rate Constant given Dissociation Rate Constant
kf=(krKD)

How to Evaluate Reverse Rate Constant in Enzymatic Reaction Mechanism?

Reverse Rate Constant in Enzymatic Reaction Mechanism evaluator uses Reverse Rate Constant = (Forward Rate Constant*Substrate Concentration*(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration))/Enzyme Substrate Complex Concentration to evaluate the Reverse Rate Constant, The Reverse rate constant in enzymatic reaction mechanism formula is defined as the relation with the rate constant of forward reaction and with the concentration of enzyme and substrate. Reverse Rate Constant is denoted by kr symbol.

How to evaluate Reverse Rate Constant in Enzymatic Reaction Mechanism using this online evaluator? To use this online evaluator for Reverse Rate Constant in Enzymatic Reaction Mechanism, enter Forward Rate Constant (kf), Substrate Concentration (S), Initial Enzyme Concentration ([E0]) & Enzyme Substrate Complex Concentration (ES) and hit the calculate button.

FAQs on Reverse Rate Constant in Enzymatic Reaction Mechanism

What is the formula to find Reverse Rate Constant in Enzymatic Reaction Mechanism?
The formula of Reverse Rate Constant in Enzymatic Reaction Mechanism is expressed as Reverse Rate Constant = (Forward Rate Constant*Substrate Concentration*(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration))/Enzyme Substrate Complex Concentration. Here is an example- 0.09315 = (6.9*1500*(100000-10000))/10000.
How to calculate Reverse Rate Constant in Enzymatic Reaction Mechanism?
With Forward Rate Constant (kf), Substrate Concentration (S), Initial Enzyme Concentration ([E0]) & Enzyme Substrate Complex Concentration (ES) we can find Reverse Rate Constant in Enzymatic Reaction Mechanism using the formula - Reverse Rate Constant = (Forward Rate Constant*Substrate Concentration*(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration))/Enzyme Substrate Complex Concentration.
What are the other ways to Calculate Reverse Rate Constant?
Here are the different ways to Calculate Reverse Rate Constant-
  • Reverse Rate Constant=(Dissociation Rate Constant*Forward Rate Constant)OpenImg
  • Reverse Rate Constant=((Forward Rate Constant*Catalyst Concentration*Substrate Concentration)/Enzyme Substrate Complex Concentration)-Catalytic Rate ConstantOpenImg
  • Reverse Rate Constant=(Michaelis Constant*Forward Rate Constant)-Catalytic Rate ConstantOpenImg
Can the Reverse Rate Constant in Enzymatic Reaction Mechanism be negative?
Yes, the Reverse Rate Constant in Enzymatic Reaction Mechanism, measured in Reaction Rate can be negative.
Which unit is used to measure Reverse Rate Constant in Enzymatic Reaction Mechanism?
Reverse Rate Constant in Enzymatic Reaction Mechanism is usually measured using the Mole per Liter Second[mol/L*s] for Reaction Rate. Millimole per Liter Second[mol/L*s], Mole per Cubic Meter Second[mol/L*s] are the few other units in which Reverse Rate Constant in Enzymatic Reaction Mechanism can be measured.
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