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The Retention Volume is defined as the volume of the mobile phase required to elute the solute from the column. Check FAQs
VR=V0(Rf+1)
VR - Retention Volume?V0 - Void Volume?Rf - Retention Factor?

Retention Volume using Retention Factor Example

With values
With units
Only example

Here is how the Retention Volume using Retention Factor equation looks like with Values.

Here is how the Retention Volume using Retention Factor equation looks like with Units.

Here is how the Retention Volume using Retention Factor equation looks like.

0.0616Edit=5.6Edit(10Edit+1)
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Retention Volume using Retention Factor Solution

Follow our step by step solution on how to calculate Retention Volume using Retention Factor?

FIRST Step Consider the formula
VR=V0(Rf+1)
Next Step Substitute values of Variables
VR=5.6mL(10+1)
Next Step Convert Units
VR=5.6E-6(10+1)
Next Step Prepare to Evaluate
VR=5.6E-6(10+1)
Next Step Evaluate
VR=6.16E-05
LAST Step Convert to Output's Unit
VR=0.0616L

Retention Volume using Retention Factor Formula Elements

Variables
Retention Volume
The Retention Volume is defined as the volume of the mobile phase required to elute the solute from the column.
Symbol: VR
Measurement: VolumeUnit: L
Note: Value can be positive or negative.
Void Volume
Void Volume is the volume of mobile phase in a column.
Symbol: V0
Measurement: VolumeUnit: mL
Note: Value should be greater than 0.
Retention Factor
Retention Factor is defined as the ratio of the distance traveled by the center of a spot to the distance traveled by the solvent front.
Symbol: Rf
Measurement: NAUnit: Unitless
Note: Value should be greater than 0.

Other Formulas to find Retention Volume

​Go Retention Volume using Column Efficiency
VR=Wb(N16)

Other formulas in Osmolality category

​Go Increase of Splicing Potential by Wild-type Sequence
SPi=log10(ωincrease)
​Go Decrease of Splicing Potential by Mutant Sequence
SPi=-log10(ω)
​Go Plasma Osmolality
Oplasma=(2Sodiumplasma)
​Go Plasma Concentration using Plasma Osmolality
Sodiumplasma=Oplasma2

How to Evaluate Retention Volume using Retention Factor?

Retention Volume using Retention Factor evaluator uses Retention Volume = Void Volume*(Retention Factor+1) to evaluate the Retention Volume, The Retention Volume using Retention Factor formula is defined as the volume that has passed through the column since the target molecule was introduced onto the column. Retention Volume is denoted by VR symbol.

How to evaluate Retention Volume using Retention Factor using this online evaluator? To use this online evaluator for Retention Volume using Retention Factor, enter Void Volume (V0) & Retention Factor (Rf) and hit the calculate button.

FAQs on Retention Volume using Retention Factor

What is the formula to find Retention Volume using Retention Factor?
The formula of Retention Volume using Retention Factor is expressed as Retention Volume = Void Volume*(Retention Factor+1). Here is an example- 61.6 = 5.6E-06*(10+1).
How to calculate Retention Volume using Retention Factor?
With Void Volume (V0) & Retention Factor (Rf) we can find Retention Volume using Retention Factor using the formula - Retention Volume = Void Volume*(Retention Factor+1).
What are the other ways to Calculate Retention Volume?
Here are the different ways to Calculate Retention Volume-
  • Retention Volume=Width of Chromatographic Peak*(sqrt(Column Efficiency/16))OpenImg
Can the Retention Volume using Retention Factor be negative?
Yes, the Retention Volume using Retention Factor, measured in Volume can be negative.
Which unit is used to measure Retention Volume using Retention Factor?
Retention Volume using Retention Factor is usually measured using the Liter[L] for Volume. Cubic Meter[L], Cubic Centimeter[L], Cubic Millimeter[L] are the few other units in which Retention Volume using Retention Factor can be measured.
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