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Retention Volume using Column Efficiency Formula

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The Retention Volume is defined as the volume of the mobile phase required to elute the solute from the column. Check FAQs

V R = W b \cdot (\sqrt{\frac{N}{16}})

V_R - Retention Volume?W_b - Width of Chromatographic Peak?N - Column Efficiency?

Retention Volume using Column Efficiency Example

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Here is how the Retention Volume using Column Efficiency equation looks like with Values.

Here is how the Retention Volume using Column Efficiency equation looks like with Units.

Here is how the Retention Volume using Column Efficiency equation looks like.

14.6575 = 25 \cdot (\sqrt{\frac{5.5}{16}})

14.6575L = 25mm \cdot (\sqrt{\frac{5.5}{16}})

14.6575 = 25 \cdot (\sqrt{\frac{5.5}{16}})

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Retention Volume using Column Efficiency Solution

Follow our step by step solution on how to calculate Retention Volume using Column Efficiency?

FIRST Step Consider the formula

V R = W b \cdot (\sqrt{\frac{N}{16}})

Next Step Substitute values of Variables

∴

V R = 25mm \cdot (\sqrt{\frac{5.5}{16}})

Next Step Convert Units

∴

V R = 0.025m \cdot (\sqrt{\frac{5.5}{16}})

Next Step Prepare to Evaluate

∴

V R = 0.025 \cdot (\sqrt{\frac{5.5}{16}})

Next Step Evaluate

∴

V R = 0.0146575492494482m³

Next Step Convert to Output's Unit

∴

V R = 14.6575492494482L

LAST Step Rounding Answer

∴

V R = 14.6575L

Retention Volume using Column Efficiency Formula Elements

Variables

Functions

Retention Volume

The Retention Volume is defined as the volume of the mobile phase required to elute the solute from the column.

Symbol: V_R

Measurement: VolumeUnit: L

Note: Value can be positive or negative.

Formulas to find Retention Volume

Width of Chromatographic Peak

Width of Chromatographic Peak is the distance between points where lines tangent to the peak's left and right inflection points intersect the baseline.

Symbol: W_b

Measurement: LengthUnit: mm

Note: Value should be greater than 0.

Formulas to find Width of Chromatographic Peak

Column Efficiency

Column Efficiency, also known as plate count, is a measure of the dispersion of a peak.

Symbol: N

Measurement: NAUnit: Unitless

Note: Value should be greater than 0.

Formulas to find Column Efficiency

sqrt

A square root function is a function that takes a non-negative number as an input and returns the square root of the given input number.

Syntax: sqrt(Number)

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How to Evaluate Retention Volume using Column Efficiency?

Retention Volume using Column Efficiency evaluator uses Retention Volume = Width of Chromatographic Peak*(sqrt(Column Efficiency/16)) to evaluate the Retention Volume, The Retention Volume using Column Efficiency formula is defined as the volume of eluent carrier gas admitted to the column between the injection of the sample and the emergence of the peak maximum of the specified component. Retention Volume is denoted by V_R symbol.

How to evaluate Retention Volume using Column Efficiency using this online evaluator? To use this online evaluator for Retention Volume using Column Efficiency, enter Width of Chromatographic Peak (W_b) & Column Efficiency (N) and hit the calculate button.

FAQs on Retention Volume using Column Efficiency

What is the formula to find Retention Volume using Column Efficiency?

The formula of Retention Volume using Column Efficiency is expressed as Retention Volume = Width of Chromatographic Peak*(sqrt(Column Efficiency/16)). Here is an example- 14657.55 = 0.025*(sqrt(5.5/16)).

How to calculate Retention Volume using Column Efficiency?

With Width of Chromatographic Peak (W_b) & Column Efficiency (N) we can find Retention Volume using Column Efficiency using the formula - Retention Volume = Width of Chromatographic Peak*(sqrt(Column Efficiency/16)). This formula also uses Square Root (sqrt) function(s).

What are the other ways to Calculate Retention Volume?

Here are the different ways to Calculate Retention Volume-

Retention Volume=Void Volume*(Retention Factor+1)

Can the Retention Volume using Column Efficiency be negative?

Yes, the Retention Volume using Column Efficiency, measured in Volume can be negative.

Which unit is used to measure Retention Volume using Column Efficiency?

Retention Volume using Column Efficiency is usually measured using the Liter[L] for Volume. Cubic Meter[L], Cubic Centimeter[L], Cubic Millimeter[L] are the few other units in which Retention Volume using Column Efficiency can be measured.

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