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Michaelis Constant given Modifying Factor in Michaelis Menten Equation Formula

GoMichaelis Constant from Michaelis Menten kinetics equation Formula

GoMichaelis Constant given Catalytic Rate Constant and Initial Enzyme Concentration Formula

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The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system. Check FAQs

K M = \frac{S \cdot ((\frac{1}{α'}) \cdot V max) - V 0}{(\frac{α}{α'}) \cdot S}

K_M - Michaelis Constant?S - Substrate Concentration?α^' - Enzyme Substrate Modifying Factor?V_max - Maximum Rate?V₀ - Initial Reaction Rate?α - Enzyme Modifying Factor?

Michaelis Constant given Modifying Factor in Michaelis Menten Equation Example

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Here is how the Michaelis Constant given Modifying Factor in Michaelis Menten Equation equation looks like with Values.

Here is how the Michaelis Constant given Modifying Factor in Michaelis Menten Equation equation looks like with Units.

Here is how the Michaelis Constant given Modifying Factor in Michaelis Menten Equation equation looks like.

7.9999 = \frac{1.5 \cdot ((\frac{1}{2}) \cdot 40) - 0.45}{(\frac{5}{2}) \cdot 1.5}

7.9999mol/L = \frac{1.5mol/L \cdot ((\frac{1}{2}) \cdot 40mol/L*s) - 0.45mol/L*s}{(\frac{5}{2}) \cdot 1.5mol/L}

7.9999 = \frac{1.5 \cdot ((\frac{1}{2}) \cdot 40) - 0.45}{(\frac{5}{2}) \cdot 1.5}

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Michaelis Constant given Modifying Factor in Michaelis Menten Equation Solution

Follow our step by step solution on how to calculate Michaelis Constant given Modifying Factor in Michaelis Menten Equation?

FIRST Step Consider the formula

K M = \frac{S \cdot ((\frac{1}{α'}) \cdot V max) - V 0}{(\frac{α}{α'}) \cdot S}

Next Step Substitute values of Variables

∴

K M = \frac{1.5mol/L \cdot ((\frac{1}{2}) \cdot 40mol/L*s) - 0.45mol/L*s}{(\frac{5}{2}) \cdot 1.5mol/L}

Next Step Convert Units

∴

K M = \frac{1500mol/m³ \cdot ((\frac{1}{2}) \cdot 40000mol/m³*s) - 450mol/m³*s}{(\frac{5}{2}) \cdot 1500mol/m³}

Next Step Prepare to Evaluate

∴

K M = \frac{1500 \cdot ((\frac{1}{2}) \cdot 40000) - 450}{(\frac{5}{2}) \cdot 1500}

Next Step Evaluate

∴

K M = 7999.88mol/m³

Next Step Convert to Output's Unit

∴

K M = 7.99988mol/L

LAST Step Rounding Answer

∴

K M = 7.9999mol/L

Michaelis Constant given Modifying Factor in Michaelis Menten Equation Formula Elements

Variables

Michaelis Constant

The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.

Symbol: K_M

Measurement: Molar ConcentrationUnit: mol/L

Note: Value can be positive or negative.

Formulas to find Michaelis Constant

Substrate Concentration

The Substrate Concentration is the number of moles of substrate per liter solution.

Symbol: S

Measurement: Molar ConcentrationUnit: mol/L

Note: Value can be positive or negative.

Formulas to find Substrate Concentration

Enzyme Substrate Modifying Factor

The Enzyme Substrate Modifying Factor is defined by the inhibitor concentration and the dissociation constant of the enzyme-substrate complex.

Symbol: α^'

Measurement: NAUnit: Unitless

Note: Value can be positive or negative.

Formulas to find Enzyme Substrate Modifying Factor

Maximum Rate

The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration.

Symbol: V_max

Measurement: Reaction RateUnit: mol/L*s

Note: Value can be positive or negative.

Formulas to find Maximum Rate

Initial Reaction Rate

The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place.

Symbol: V₀

Measurement: Reaction RateUnit: mol/L*s

Note: Value can be positive or negative.

Formulas to find Initial Reaction Rate

Enzyme Modifying Factor

The Enzyme Modifying Factor is defined by the inhibitor concentration and the dissociation constants of enzyme.

Symbol: α

Measurement: NAUnit: Unitless

Note: Value can be positive or negative.

Formulas to find Enzyme Modifying Factor

Other Formulas to find Michaelis Constant

Go Michaelis Constant from Michaelis Menten kinetics equation

K M = S \cdot (\frac{V max - V 0}{V 0})

Go Michaelis Constant given Catalytic Rate Constant and Initial Enzyme Concentration

K M = \frac{S \cdot ((k cat \cdot [E 0]) - V 0)}{V 0}

Go Michaelis Constant at Low Substrate Concentration

K M = \frac{k cat \cdot [E 0] \cdot S}{V 0}

Go Michaelis Constant given Maximum Rate at Low Substrate Concentration

K M = \frac{V max \cdot S}{V 0}

Other formulas in Michaelis Menten Kinetics Equation category

Go Maximum Rate of System from Michaelis Menten Kinetics equation

V max = \frac{V 0 \cdot (K M + S)}{S}

Go Substrate Concentration from Michaelis Menten Kinetics Equation

S = \frac{K M \cdot V 0}{V max - V 0}

Go Catalytic Rate Constant from Michaelis Menten Kinetics Equation

k cat_MM = \frac{V 0 \cdot (K M + S)}{[E 0] \cdot S}

Go Enzyme Concentration from Michaelis Menten Kinetics equation

[E i] = \frac{V 0 \cdot (K M + S)}{k cat \cdot S}

How to Evaluate Michaelis Constant given Modifying Factor in Michaelis Menten Equation?

Michaelis Constant given Modifying Factor in Michaelis Menten Equation evaluator uses Michaelis Constant = (Substrate Concentration*((1/Enzyme Substrate Modifying Factor)*Maximum Rate)-Initial Reaction Rate)/((Enzyme Modifying Factor/Enzyme Substrate Modifying Factor)*Substrate Concentration) to evaluate the Michaelis Constant, The Michaelis constant given modifying factor in Michaelis Menten equation formula is defined as the enzyme activity under various substrate and inhibitor concentrations, to obtain a modified Michaelis–Menten equation. Michaelis Constant is denoted by K_M symbol.

How to evaluate Michaelis Constant given Modifying Factor in Michaelis Menten Equation using this online evaluator? To use this online evaluator for Michaelis Constant given Modifying Factor in Michaelis Menten Equation, enter Substrate Concentration (S), Enzyme Substrate Modifying Factor (α^'), Maximum Rate (V_max), Initial Reaction Rate (V₀) & Enzyme Modifying Factor (α) and hit the calculate button.

FAQs on Michaelis Constant given Modifying Factor in Michaelis Menten Equation

What is the formula to find Michaelis Constant given Modifying Factor in Michaelis Menten Equation?

The formula of Michaelis Constant given Modifying Factor in Michaelis Menten Equation is expressed as Michaelis Constant = (Substrate Concentration*((1/Enzyme Substrate Modifying Factor)*Maximum Rate)-Initial Reaction Rate)/((Enzyme Modifying Factor/Enzyme Substrate Modifying Factor)*Substrate Concentration). Here is an example- 0.008 = (1500*((1/2)*40000)-450)/((5/2)*1500).

How to calculate Michaelis Constant given Modifying Factor in Michaelis Menten Equation?

With Substrate Concentration (S), Enzyme Substrate Modifying Factor (α^'), Maximum Rate (V_max), Initial Reaction Rate (V₀) & Enzyme Modifying Factor (α) we can find Michaelis Constant given Modifying Factor in Michaelis Menten Equation using the formula - Michaelis Constant = (Substrate Concentration*((1/Enzyme Substrate Modifying Factor)*Maximum Rate)-Initial Reaction Rate)/((Enzyme Modifying Factor/Enzyme Substrate Modifying Factor)*Substrate Concentration).

What are the other ways to Calculate Michaelis Constant?

Here are the different ways to Calculate Michaelis Constant-

Michaelis Constant=Substrate Concentration*((Maximum Rate-Initial Reaction Rate)/Initial Reaction Rate)
Michaelis Constant=(Substrate Concentration*((Catalytic Rate Constant*Initial Enzyme Concentration)-Initial Reaction Rate))/Initial Reaction Rate
Michaelis Constant=(Catalytic Rate Constant*Initial Enzyme Concentration*Substrate Concentration)/Initial Reaction Rate

Can the Michaelis Constant given Modifying Factor in Michaelis Menten Equation be negative?

Yes, the Michaelis Constant given Modifying Factor in Michaelis Menten Equation, measured in Molar Concentration can be negative.

Which unit is used to measure Michaelis Constant given Modifying Factor in Michaelis Menten Equation?

Michaelis Constant given Modifying Factor in Michaelis Menten Equation is usually measured using the Mole per Liter[mol/L] for Molar Concentration. Mole per Cubic Meter[mol/L], Mole per Cubic Millimeter[mol/L], Kilomole per Cubic Meter[mol/L] are the few other units in which Michaelis Constant given Modifying Factor in Michaelis Menten Equation can be measured.

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Michaelis Constant given Modifying Factor in Michaelis Menten Equation Formula

​GoMichaelis Constant from Michaelis Menten kinetics equation Formula

​GoMichaelis Constant given Catalytic Rate Constant and Initial Enzyme Concentration Formula

Michaelis Constant given Modifying Factor in Michaelis Menten Equation Example

Michaelis Constant given Modifying Factor in Michaelis Menten Equation Solution

Michaelis Constant given Modifying Factor in Michaelis Menten Equation Formula Elements

Other Formulas to find Michaelis Constant

Other formulas in Michaelis Menten Kinetics Equation category

How to Evaluate Michaelis Constant given Modifying Factor in Michaelis Menten Equation?

FAQs on Michaelis Constant given Modifying Factor in Michaelis Menten Equation

Variable Name

GoMichaelis Constant from Michaelis Menten kinetics equation Formula

GoMichaelis Constant given Catalytic Rate Constant and Initial Enzyme Concentration Formula