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The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system. Check FAQs
KM=VmaxSV0
KM - Michaelis Constant?Vmax - Maximum Rate?S - Substrate Concentration?V0 - Initial Reaction Rate?

Michaelis Constant given Maximum Rate at Low Substrate Concentration Example

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With units
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Here is how the Michaelis Constant given Maximum Rate at Low Substrate Concentration equation looks like with Values.

Here is how the Michaelis Constant given Maximum Rate at Low Substrate Concentration equation looks like with Units.

Here is how the Michaelis Constant given Maximum Rate at Low Substrate Concentration equation looks like.

133.3333Edit=40Edit1.5Edit0.45Edit
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Michaelis Constant given Maximum Rate at Low Substrate Concentration Solution

Follow our step by step solution on how to calculate Michaelis Constant given Maximum Rate at Low Substrate Concentration?

FIRST Step Consider the formula
KM=VmaxSV0
Next Step Substitute values of Variables
KM=40mol/L*s1.5mol/L0.45mol/L*s
Next Step Convert Units
KM=40000mol/m³*s1500mol/m³450mol/m³*s
Next Step Prepare to Evaluate
KM=400001500450
Next Step Evaluate
KM=133333.333333333mol/m³
Next Step Convert to Output's Unit
KM=133.333333333333mol/L
LAST Step Rounding Answer
KM=133.3333mol/L

Michaelis Constant given Maximum Rate at Low Substrate Concentration Formula Elements

Variables
Michaelis Constant
The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.
Symbol: KM
Measurement: Molar ConcentrationUnit: mol/L
Note: Value can be positive or negative.
Maximum Rate
The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration.
Symbol: Vmax
Measurement: Reaction RateUnit: mol/L*s
Note: Value can be positive or negative.
Substrate Concentration
The Substrate Concentration is the number of moles of substrate per liter solution.
Symbol: S
Measurement: Molar ConcentrationUnit: mol/L
Note: Value can be positive or negative.
Initial Reaction Rate
The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place.
Symbol: V0
Measurement: Reaction RateUnit: mol/L*s
Note: Value can be positive or negative.

Other Formulas to find Michaelis Constant

​Go Michaelis Constant from Michaelis Menten kinetics equation
KM=S(Vmax-V0V0)
​Go Michaelis Constant given Catalytic Rate Constant and Initial Enzyme Concentration
KM=S((kcat[E0])-V0)V0
​Go Michaelis Constant at Low Substrate Concentration
KM=kcat[E0]SV0
​Go Michaelis Constant given Forward, Reverse, and Catalytic Rate Constants
KM=kr+kcatkf

Other formulas in Michaelis Menten Kinetics Equation category

​Go Maximum Rate of System from Michaelis Menten Kinetics equation
Vmax=V0(KM+S)S
​Go Substrate Concentration from Michaelis Menten Kinetics Equation
S=KMV0Vmax-V0
​Go Catalytic Rate Constant from Michaelis Menten Kinetics Equation
kcat_MM=V0(KM+S)[E0]S
​Go Enzyme Concentration from Michaelis Menten Kinetics equation
[Ei]=V0(KM+S)kcatS

How to Evaluate Michaelis Constant given Maximum Rate at Low Substrate Concentration?

Michaelis Constant given Maximum Rate at Low Substrate Concentration evaluator uses Michaelis Constant = (Maximum Rate*Substrate Concentration)/Initial Reaction Rate to evaluate the Michaelis Constant, The Michaelis constant given maximum rate at low substrate concentration formula is defined as the relation with the initial and maximum rate of the system and with the substrate concentration. Here substrate concentration is very less i.e negligible in comparison to Michaelis constant. Michaelis Constant is denoted by KM symbol.

How to evaluate Michaelis Constant given Maximum Rate at Low Substrate Concentration using this online evaluator? To use this online evaluator for Michaelis Constant given Maximum Rate at Low Substrate Concentration, enter Maximum Rate (Vmax), Substrate Concentration (S) & Initial Reaction Rate (V0) and hit the calculate button.

FAQs on Michaelis Constant given Maximum Rate at Low Substrate Concentration

What is the formula to find Michaelis Constant given Maximum Rate at Low Substrate Concentration?
The formula of Michaelis Constant given Maximum Rate at Low Substrate Concentration is expressed as Michaelis Constant = (Maximum Rate*Substrate Concentration)/Initial Reaction Rate. Here is an example- 0.133333 = (40000*1500)/450.
How to calculate Michaelis Constant given Maximum Rate at Low Substrate Concentration?
With Maximum Rate (Vmax), Substrate Concentration (S) & Initial Reaction Rate (V0) we can find Michaelis Constant given Maximum Rate at Low Substrate Concentration using the formula - Michaelis Constant = (Maximum Rate*Substrate Concentration)/Initial Reaction Rate.
What are the other ways to Calculate Michaelis Constant?
Here are the different ways to Calculate Michaelis Constant-
  • Michaelis Constant=Substrate Concentration*((Maximum Rate-Initial Reaction Rate)/Initial Reaction Rate)OpenImg
  • Michaelis Constant=(Substrate Concentration*((Catalytic Rate Constant*Initial Enzyme Concentration)-Initial Reaction Rate))/Initial Reaction RateOpenImg
  • Michaelis Constant=(Catalytic Rate Constant*Initial Enzyme Concentration*Substrate Concentration)/Initial Reaction RateOpenImg
Can the Michaelis Constant given Maximum Rate at Low Substrate Concentration be negative?
Yes, the Michaelis Constant given Maximum Rate at Low Substrate Concentration, measured in Molar Concentration can be negative.
Which unit is used to measure Michaelis Constant given Maximum Rate at Low Substrate Concentration?
Michaelis Constant given Maximum Rate at Low Substrate Concentration is usually measured using the Mole per Liter[mol/L] for Molar Concentration. Mole per Cubic Meter[mol/L], Mole per Cubic Millimeter[mol/L], Kilomole per Cubic Meter[mol/L] are the few other units in which Michaelis Constant given Maximum Rate at Low Substrate Concentration can be measured.
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