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Michaelis Constant from Michaelis Menten kinetics equation Formula

GoMichaelis Constant given Catalytic Rate Constant and Initial Enzyme Concentration Formula

GoMichaelis Constant at Low Substrate Concentration Formula

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The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system. Check FAQs

K M = S \cdot (\frac{V max - V 0}{V 0})

K_M - Michaelis Constant?S - Substrate Concentration?V_max - Maximum Rate?V₀ - Initial Reaction Rate?

Michaelis Constant from Michaelis Menten kinetics equation Example

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Here is how the Michaelis Constant from Michaelis Menten kinetics equation equation looks like with Values.

Here is how the Michaelis Constant from Michaelis Menten kinetics equation equation looks like with Units.

Here is how the Michaelis Constant from Michaelis Menten kinetics equation equation looks like.

131.8333 = 1.5 \cdot (\frac{40 - 0.45}{0.45})

131.8333mol/L = 1.5mol/L \cdot (\frac{40mol/L*s - 0.45mol/L*s}{0.45mol/L*s})

131.8333 = 1.5 \cdot (\frac{40 - 0.45}{0.45})

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Michaelis Constant from Michaelis Menten kinetics equation Solution

Follow our step by step solution on how to calculate Michaelis Constant from Michaelis Menten kinetics equation?

FIRST Step Consider the formula

K M = S \cdot (\frac{V max - V 0}{V 0})

Next Step Substitute values of Variables

∴

K M = 1.5mol/L \cdot (\frac{40mol/L*s - 0.45mol/L*s}{0.45mol/L*s})

Next Step Convert Units

∴

K M = 1500mol/m³ \cdot (\frac{40000mol/m³*s - 450mol/m³*s}{450mol/m³*s})

Next Step Prepare to Evaluate

∴

K M = 1500 \cdot (\frac{40000 - 450}{450})

Next Step Evaluate

∴

K M = 131833.333333333mol/m³

Next Step Convert to Output's Unit

∴

K M = 131.833333333333mol/L

LAST Step Rounding Answer

∴

K M = 131.8333mol/L

Michaelis Constant from Michaelis Menten kinetics equation Formula Elements

Variables

Michaelis Constant

The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.

Symbol: K_M

Measurement: Molar ConcentrationUnit: mol/L

Note: Value can be positive or negative.

Formulas to find Michaelis Constant

Substrate Concentration

The Substrate Concentration is the number of moles of substrate per liter solution.

Symbol: S

Measurement: Molar ConcentrationUnit: mol/L

Note: Value can be positive or negative.

Formulas to find Substrate Concentration

Maximum Rate

The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration.

Symbol: V_max

Measurement: Reaction RateUnit: mol/L*s

Note: Value can be positive or negative.

Formulas to find Maximum Rate

Initial Reaction Rate

The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place.

Symbol: V₀

Measurement: Reaction RateUnit: mol/L*s

Note: Value can be positive or negative.

Formulas to find Initial Reaction Rate

Other Formulas to find Michaelis Constant

Go Michaelis Constant given Catalytic Rate Constant and Initial Enzyme Concentration

K M = \frac{S \cdot ((k cat \cdot [E 0]) - V 0)}{V 0}

Go Michaelis Constant at Low Substrate Concentration

K M = \frac{k cat \cdot [E 0] \cdot S}{V 0}

Go Michaelis Constant given Maximum Rate at Low Substrate Concentration

K M = \frac{V max \cdot S}{V 0}

Go Michaelis Constant given Forward, Reverse, and Catalytic Rate Constants

K M = \frac{k r + k cat}{k f}

Other formulas in Michaelis Menten Kinetics Equation category

Go Maximum Rate of System from Michaelis Menten Kinetics equation

V max = \frac{V 0 \cdot (K M + S)}{S}

Go Substrate Concentration from Michaelis Menten Kinetics Equation

S = \frac{K M \cdot V 0}{V max - V 0}

Go Catalytic Rate Constant from Michaelis Menten Kinetics Equation

k cat_MM = \frac{V 0 \cdot (K M + S)}{[E 0] \cdot S}

Go Enzyme Concentration from Michaelis Menten Kinetics equation

[E i] = \frac{V 0 \cdot (K M + S)}{k cat \cdot S}

How to Evaluate Michaelis Constant from Michaelis Menten kinetics equation?

Michaelis Constant from Michaelis Menten kinetics equation evaluator uses Michaelis Constant = Substrate Concentration*((Maximum Rate-Initial Reaction Rate)/Initial Reaction Rate) to evaluate the Michaelis Constant, The Michaelis constant from Michaelis Menten kinetics equation formula is defined as the relation with the substrate concentration and initial and maximum rate achieved by the system. Michaelis Constant is denoted by K_M symbol.

How to evaluate Michaelis Constant from Michaelis Menten kinetics equation using this online evaluator? To use this online evaluator for Michaelis Constant from Michaelis Menten kinetics equation, enter Substrate Concentration (S), Maximum Rate (V_max) & Initial Reaction Rate (V₀) and hit the calculate button.

FAQs on Michaelis Constant from Michaelis Menten kinetics equation

What is the formula to find Michaelis Constant from Michaelis Menten kinetics equation?

The formula of Michaelis Constant from Michaelis Menten kinetics equation is expressed as Michaelis Constant = Substrate Concentration*((Maximum Rate-Initial Reaction Rate)/Initial Reaction Rate). Here is an example- 0.131833 = 1500*((40000-450)/450).

How to calculate Michaelis Constant from Michaelis Menten kinetics equation?

With Substrate Concentration (S), Maximum Rate (V_max) & Initial Reaction Rate (V₀) we can find Michaelis Constant from Michaelis Menten kinetics equation using the formula - Michaelis Constant = Substrate Concentration*((Maximum Rate-Initial Reaction Rate)/Initial Reaction Rate).

What are the other ways to Calculate Michaelis Constant?

Here are the different ways to Calculate Michaelis Constant-

Michaelis Constant=(Substrate Concentration*((Catalytic Rate Constant*Initial Enzyme Concentration)-Initial Reaction Rate))/Initial Reaction Rate
Michaelis Constant=(Catalytic Rate Constant*Initial Enzyme Concentration*Substrate Concentration)/Initial Reaction Rate
Michaelis Constant=(Maximum Rate*Substrate Concentration)/Initial Reaction Rate

Can the Michaelis Constant from Michaelis Menten kinetics equation be negative?

Yes, the Michaelis Constant from Michaelis Menten kinetics equation, measured in Molar Concentration can be negative.

Which unit is used to measure Michaelis Constant from Michaelis Menten kinetics equation?

Michaelis Constant from Michaelis Menten kinetics equation is usually measured using the Mole per Liter[mol/L] for Molar Concentration. Mole per Cubic Meter[mol/L], Mole per Cubic Millimeter[mol/L], Kilomole per Cubic Meter[mol/L] are the few other units in which Michaelis Constant from Michaelis Menten kinetics equation can be measured.

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Michaelis Constant from Michaelis Menten kinetics equation Formula

​GoMichaelis Constant given Catalytic Rate Constant and Initial Enzyme Concentration Formula

​GoMichaelis Constant at Low Substrate Concentration Formula

Michaelis Constant from Michaelis Menten kinetics equation Example

Michaelis Constant from Michaelis Menten kinetics equation Solution

Michaelis Constant from Michaelis Menten kinetics equation Formula Elements

Other Formulas to find Michaelis Constant

Other formulas in Michaelis Menten Kinetics Equation category

How to Evaluate Michaelis Constant from Michaelis Menten kinetics equation?

FAQs on Michaelis Constant from Michaelis Menten kinetics equation

Variable Name

GoMichaelis Constant given Catalytic Rate Constant and Initial Enzyme Concentration Formula

GoMichaelis Constant at Low Substrate Concentration Formula