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Michaelis Constant at Low Substrate Concentration Formula

GoMichaelis Constant from Michaelis Menten kinetics equation Formula

GoMichaelis Constant given Catalytic Rate Constant and Initial Enzyme Concentration Formula

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The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system. Check FAQs

K M = \frac{k cat \cdot [E 0] \cdot S}{V 0}

K_M - Michaelis Constant?k_cat - Catalytic Rate Constant?[E₀] - Initial Enzyme Concentration?S - Substrate Concentration?V₀ - Initial Reaction Rate?

Michaelis Constant at Low Substrate Concentration Example

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Here is how the Michaelis Constant at Low Substrate Concentration equation looks like with Values.

Here is how the Michaelis Constant at Low Substrate Concentration equation looks like with Units.

Here is how the Michaelis Constant at Low Substrate Concentration equation looks like.

216.6667 = \frac{0.65 \cdot 100 \cdot 1.5}{0.45}

216.6667mol/L = \frac{0.65s⁻¹ \cdot 100mol/L \cdot 1.5mol/L}{0.45mol/L*s}

216.6667 = \frac{0.65 \cdot 100 \cdot 1.5}{0.45}

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Enzyme Kinetics » fx Michaelis Constant at Low Substrate Concentration

Michaelis Constant at Low Substrate Concentration Solution

Follow our step by step solution on how to calculate Michaelis Constant at Low Substrate Concentration?

FIRST Step Consider the formula

K M = \frac{k cat \cdot [E 0] \cdot S}{V 0}

Next Step Substitute values of Variables

∴

K M = \frac{0.65s⁻¹ \cdot 100mol/L \cdot 1.5mol/L}{0.45mol/L*s}

Next Step Convert Units

∴

K M = \frac{0.65s⁻¹ \cdot 100000mol/m³ \cdot 1500mol/m³}{450mol/m³*s}

Next Step Prepare to Evaluate

∴

K M = \frac{0.65 \cdot 100000 \cdot 1500}{450}

Next Step Evaluate

∴

K M = 216666.666666667mol/m³

Next Step Convert to Output's Unit

∴

K M = 216.666666666667mol/L

LAST Step Rounding Answer

∴

K M = 216.6667mol/L

Michaelis Constant at Low Substrate Concentration Formula Elements

Variables

Michaelis Constant

The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.

Symbol: K_M

Measurement: Molar ConcentrationUnit: mol/L

Note: Value can be positive or negative.

Formulas to find Michaelis Constant

Catalytic Rate Constant

The Catalytic Rate Constant is defined as the rate constant for conversion of the enzyme-substrate complex to enzyme and product.

Symbol: k_cat

Measurement: First Order Reaction Rate ConstantUnit: s⁻¹

Note: Value can be positive or negative.

Formulas to find Catalytic Rate Constant

Initial Enzyme Concentration

The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction.

Symbol: [E₀]

Measurement: Molar ConcentrationUnit: mol/L

Note: Value should be greater than 0.

Formulas to find Initial Enzyme Concentration

Substrate Concentration

The Substrate Concentration is the number of moles of substrate per liter solution.

Symbol: S

Measurement: Molar ConcentrationUnit: mol/L

Note: Value can be positive or negative.

Formulas to find Substrate Concentration

Initial Reaction Rate

The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place.

Symbol: V₀

Measurement: Reaction RateUnit: mol/L*s

Note: Value can be positive or negative.

Formulas to find Initial Reaction Rate

Other Formulas to find Michaelis Constant

Go Michaelis Constant from Michaelis Menten kinetics equation

K M = S \cdot (\frac{V max - V 0}{V 0})

Go Michaelis Constant given Catalytic Rate Constant and Initial Enzyme Concentration

K M = \frac{S \cdot ((k cat \cdot [E 0]) - V 0)}{V 0}

Go Michaelis Constant given Maximum Rate at Low Substrate Concentration

K M = \frac{V max \cdot S}{V 0}

Go Michaelis Constant given Forward, Reverse, and Catalytic Rate Constants

K M = \frac{k r + k cat}{k f}

Other formulas in Michaelis Menten Kinetics Equation category

Go Maximum Rate of System from Michaelis Menten Kinetics equation

V max = \frac{V 0 \cdot (K M + S)}{S}

Go Substrate Concentration from Michaelis Menten Kinetics Equation

S = \frac{K M \cdot V 0}{V max - V 0}

Go Catalytic Rate Constant from Michaelis Menten Kinetics Equation

k cat_MM = \frac{V 0 \cdot (K M + S)}{[E 0] \cdot S}

Go Enzyme Concentration from Michaelis Menten Kinetics equation

[E i] = \frac{V 0 \cdot (K M + S)}{k cat \cdot S}

How to Evaluate Michaelis Constant at Low Substrate Concentration?

Michaelis Constant at Low Substrate Concentration evaluator uses Michaelis Constant = (Catalytic Rate Constant*Initial Enzyme Concentration*Substrate Concentration)/Initial Reaction Rate to evaluate the Michaelis Constant, The Michaelis constant at low substrate concentration formula is defined as the relation of initial enzyme and substrate concentration and wth initial reaction rate. Here substrate concentration is very less i.e negligible in comparison to Michaelis constant. Michaelis Constant is denoted by K_M symbol.

How to evaluate Michaelis Constant at Low Substrate Concentration using this online evaluator? To use this online evaluator for Michaelis Constant at Low Substrate Concentration, enter Catalytic Rate Constant (k_cat), Initial Enzyme Concentration ([E₀]), Substrate Concentration (S) & Initial Reaction Rate (V₀) and hit the calculate button.

FAQs on Michaelis Constant at Low Substrate Concentration

What is the formula to find Michaelis Constant at Low Substrate Concentration?

The formula of Michaelis Constant at Low Substrate Concentration is expressed as Michaelis Constant = (Catalytic Rate Constant*Initial Enzyme Concentration*Substrate Concentration)/Initial Reaction Rate. Here is an example- 0.216667 = (0.65*100000*1500)/450.

How to calculate Michaelis Constant at Low Substrate Concentration?

With Catalytic Rate Constant (k_cat), Initial Enzyme Concentration ([E₀]), Substrate Concentration (S) & Initial Reaction Rate (V₀) we can find Michaelis Constant at Low Substrate Concentration using the formula - Michaelis Constant = (Catalytic Rate Constant*Initial Enzyme Concentration*Substrate Concentration)/Initial Reaction Rate.

What are the other ways to Calculate Michaelis Constant?

Here are the different ways to Calculate Michaelis Constant-

Michaelis Constant=Substrate Concentration*((Maximum Rate-Initial Reaction Rate)/Initial Reaction Rate)
Michaelis Constant=(Substrate Concentration*((Catalytic Rate Constant*Initial Enzyme Concentration)-Initial Reaction Rate))/Initial Reaction Rate
Michaelis Constant=(Maximum Rate*Substrate Concentration)/Initial Reaction Rate

Can the Michaelis Constant at Low Substrate Concentration be negative?

Yes, the Michaelis Constant at Low Substrate Concentration, measured in Molar Concentration can be negative.

Which unit is used to measure Michaelis Constant at Low Substrate Concentration?

Michaelis Constant at Low Substrate Concentration is usually measured using the Mole per Liter[mol/L] for Molar Concentration. Mole per Cubic Meter[mol/L], Mole per Cubic Millimeter[mol/L], Kilomole per Cubic Meter[mol/L] are the few other units in which Michaelis Constant at Low Substrate Concentration can be measured.

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Michaelis Constant at Low Substrate Concentration Formula

​GoMichaelis Constant from Michaelis Menten kinetics equation Formula

​GoMichaelis Constant given Catalytic Rate Constant and Initial Enzyme Concentration Formula

Michaelis Constant at Low Substrate Concentration Example

Michaelis Constant at Low Substrate Concentration Solution

Michaelis Constant at Low Substrate Concentration Formula Elements

Other Formulas to find Michaelis Constant

Other formulas in Michaelis Menten Kinetics Equation category

How to Evaluate Michaelis Constant at Low Substrate Concentration?

FAQs on Michaelis Constant at Low Substrate Concentration

Variable Name

GoMichaelis Constant from Michaelis Menten kinetics equation Formula

GoMichaelis Constant given Catalytic Rate Constant and Initial Enzyme Concentration Formula