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Lineweaver Burk plot Formula

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The initial reaction rate is the rate at which a chemical reaction is procceding at the very beggining, at the moment when the reactants are mixed. Check FAQs

V O = \frac{V max \cdot S conc.}{K M + S conc.}

V_O - The initial reaction rate?V_max - The maximum reaction rate?S_conc. - The substrare concentration?K_M - Michaelis Constant?

Lineweaver Burk plot Example

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Here is how the Lineweaver Burk plot equation looks like with Values.

Here is how the Lineweaver Burk plot equation looks like with Units.

Here is how the Lineweaver Burk plot equation looks like.

0.1987 = \frac{30 \cdot 20}{3 + 20}

0.1987mol/L*s = \frac{30mol/L*s \cdot 20g/L}{3mol/L + 20g/L}

0.1987 = \frac{30 \cdot 20}{3 + 20}

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Lineweaver Burk plot Solution

Follow our step by step solution on how to calculate Lineweaver Burk plot?

FIRST Step Consider the formula

V O = \frac{V max \cdot S conc.}{K M + S conc.}

Next Step Substitute values of Variables

∴

V O = \frac{30mol/L*s \cdot 20g/L}{3mol/L + 20g/L}

Next Step Convert Units

∴

V O = \frac{30000mol/m³*s \cdot 20kg/m³}{3000mol/m³ + 20kg/m³}

Next Step Prepare to Evaluate

∴

V O = \frac{30000 \cdot 20}{3000 + 20}

Next Step Evaluate

∴

V O = 198.675496688742mol/m³*s

Next Step Convert to Output's Unit

∴

V O = 0.198675496688742mol/L*s

LAST Step Rounding Answer

∴

V O = 0.1987mol/L*s

Lineweaver Burk plot Formula Elements

Variables

The initial reaction rate

The initial reaction rate is the rate at which a chemical reaction is procceding at the very beggining, at the moment when the reactants are mixed.

Symbol: V_O

Measurement: Reaction RateUnit: mol/L*s

Note: Value should be greater than 0.

Formulas to find The initial reaction rate

The maximum reaction rate

The maximum reaction rate is the maximum rate occurs in a chemical reaction.

Symbol: V_max

Measurement: Reaction RateUnit: mol/L*s

Note: Value should be greater than 0.

Formulas to find The maximum reaction rate

The substrare concentration

The substrare concentration is the concentration of the substrate which undergoes enzyme treatment.

Symbol: S_conc.

Measurement: Mass ConcentrationUnit: g/L

Note: Value should be greater than 0.

Formulas to find The substrare concentration

Michaelis Constant

The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.

Symbol: K_M

Measurement: Molar ConcentrationUnit: mol/L

Note: Value can be positive or negative.

Formulas to find Michaelis Constant

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How to Evaluate Lineweaver Burk plot?

Lineweaver Burk plot evaluator uses The initial reaction rate = (The maximum reaction rate*The substrare concentration)/(Michaelis Constant+The substrare concentration) to evaluate the The initial reaction rate, Lineweaver Burk plot also known as the double reciprocal plot, is a graphical method used to analyze enzyme kinetics data. It provides a linear representation of the Michaelis-Menten equation, making it easier to determine important kinetic parameters of enzyme-catalyzed reactions. The initial reaction rate is denoted by V_O symbol.

How to evaluate Lineweaver Burk plot using this online evaluator? To use this online evaluator for Lineweaver Burk plot, enter The maximum reaction rate (V_max), The substrare concentration (S_conc.) & Michaelis Constant (K_M) and hit the calculate button.

FAQs on Lineweaver Burk plot

What is the formula to find Lineweaver Burk plot?

The formula of Lineweaver Burk plot is expressed as The initial reaction rate = (The maximum reaction rate*The substrare concentration)/(Michaelis Constant+The substrare concentration). Here is an example- 0.000199 = (30000*20)/(3000+20).

How to calculate Lineweaver Burk plot?

With The maximum reaction rate (V_max), The substrare concentration (S_conc.) & Michaelis Constant (K_M) we can find Lineweaver Burk plot using the formula - The initial reaction rate = (The maximum reaction rate*The substrare concentration)/(Michaelis Constant+The substrare concentration).

Can the Lineweaver Burk plot be negative?

No, the Lineweaver Burk plot, measured in Reaction Rate cannot be negative.

Which unit is used to measure Lineweaver Burk plot?

Lineweaver Burk plot is usually measured using the Mole per Liter Second[mol/L*s] for Reaction Rate. Millimole per Liter Second[mol/L*s], Mole per Cubic Meter Second[mol/L*s] are the few other units in which Lineweaver Burk plot can be measured.

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Lineweaver Burk plot Formula

Lineweaver Burk plot Example

Lineweaver Burk plot Solution

Lineweaver Burk plot Formula Elements

Other formulas in Microbiology category

How to Evaluate Lineweaver Burk plot?

FAQs on Lineweaver Burk plot

Variable Name