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The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place. Check FAQs
V0=kcat[E0]SKM
V0 - Initial Reaction Rate?kcat - Catalytic Rate Constant?[E0] - Initial Enzyme Concentration?S - Substrate Concentration?KM - Michaelis Constant?

Initial Reaction Rate at Low Substrate Concentration Example

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With units
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Here is how the Initial Reaction Rate at Low Substrate Concentration equation looks like with Values.

Here is how the Initial Reaction Rate at Low Substrate Concentration equation looks like with Units.

Here is how the Initial Reaction Rate at Low Substrate Concentration equation looks like.

32.5Edit=0.65Edit100Edit1.5Edit3Edit
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Initial Reaction Rate at Low Substrate Concentration Solution

Follow our step by step solution on how to calculate Initial Reaction Rate at Low Substrate Concentration?

FIRST Step Consider the formula
V0=kcat[E0]SKM
Next Step Substitute values of Variables
V0=0.65s⁻¹100mol/L1.5mol/L3mol/L
Next Step Convert Units
V0=0.65s⁻¹100000mol/m³1500mol/m³3000mol/m³
Next Step Prepare to Evaluate
V0=0.6510000015003000
Next Step Evaluate
V0=32500mol/m³*s
LAST Step Convert to Output's Unit
V0=32.5mol/L*s

Initial Reaction Rate at Low Substrate Concentration Formula Elements

Variables
Initial Reaction Rate
The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place.
Symbol: V0
Measurement: Reaction RateUnit: mol/L*s
Note: Value can be positive or negative.
Catalytic Rate Constant
The Catalytic Rate Constant is defined as the rate constant for conversion of the enzyme-substrate complex to enzyme and product.
Symbol: kcat
Measurement: First Order Reaction Rate ConstantUnit: s⁻¹
Note: Value can be positive or negative.
Initial Enzyme Concentration
The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction.
Symbol: [E0]
Measurement: Molar ConcentrationUnit: mol/L
Note: Value should be greater than 0.
Substrate Concentration
The Substrate Concentration is the number of moles of substrate per liter solution.
Symbol: S
Measurement: Molar ConcentrationUnit: mol/L
Note: Value can be positive or negative.
Michaelis Constant
The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.
Symbol: KM
Measurement: Molar ConcentrationUnit: mol/L
Note: Value can be positive or negative.

Other Formulas to find Initial Reaction Rate

​Go Initial Reaction Rate in Michaelis Menten kinetics Equation
V0=VmaxSKM+S
​Go Initial Reaction Rate given Catalytic Rate Constant and Initial Enzyme Concentration
V0=kcat[E0]SKM+S
​Go Initial Reaction Rate at Low Substrate Concentration terms of Maximum Rate
V0=VmaxSKM
​Go Initial Reaction Rate given Catalytic Rate Constant and Dissociation Rate Constants
V0=kcat[E0]SKD+S

Other formulas in Enzyme Kinetics category

​Go Maximum Rate of System at Low Substrate Concentration
Vmax=V0KMS
​Go Initial Reaction Rate given Dissociation Rate Constant
VDRC=VmaxSKD+S
​Go Maximum Rate given Dissociation Rate Constant
Vmax_DRC=V0(KD+S)S
​Go Modifying Factor of Enzyme Substrate Complex
α'=1+(IKi')

How to Evaluate Initial Reaction Rate at Low Substrate Concentration?

Initial Reaction Rate at Low Substrate Concentration evaluator uses Initial Reaction Rate = (Catalytic Rate Constant*Initial Enzyme Concentration*Substrate Concentration)/Michaelis Constant to evaluate the Initial Reaction Rate, The Initial reaction rate at low substrate concentration formula is defined as the relation with initial enzyme concentration and the concentration of a substrate. Here substrate concentration is very less i.e negligible in comparison to Michaelis constant. Initial Reaction Rate is denoted by V0 symbol.

How to evaluate Initial Reaction Rate at Low Substrate Concentration using this online evaluator? To use this online evaluator for Initial Reaction Rate at Low Substrate Concentration, enter Catalytic Rate Constant (kcat), Initial Enzyme Concentration ([E0]), Substrate Concentration (S) & Michaelis Constant (KM) and hit the calculate button.

FAQs on Initial Reaction Rate at Low Substrate Concentration

What is the formula to find Initial Reaction Rate at Low Substrate Concentration?
The formula of Initial Reaction Rate at Low Substrate Concentration is expressed as Initial Reaction Rate = (Catalytic Rate Constant*Initial Enzyme Concentration*Substrate Concentration)/Michaelis Constant. Here is an example- 0.0325 = (0.65*100000*1500)/3000.
How to calculate Initial Reaction Rate at Low Substrate Concentration?
With Catalytic Rate Constant (kcat), Initial Enzyme Concentration ([E0]), Substrate Concentration (S) & Michaelis Constant (KM) we can find Initial Reaction Rate at Low Substrate Concentration using the formula - Initial Reaction Rate = (Catalytic Rate Constant*Initial Enzyme Concentration*Substrate Concentration)/Michaelis Constant.
What are the other ways to Calculate Initial Reaction Rate?
Here are the different ways to Calculate Initial Reaction Rate-
  • Initial Reaction Rate=(Maximum Rate*Substrate Concentration)/(Michaelis Constant+Substrate Concentration)OpenImg
  • Initial Reaction Rate=(Catalytic Rate Constant*Initial Enzyme Concentration*Substrate Concentration)/(Michaelis Constant+Substrate Concentration)OpenImg
  • Initial Reaction Rate=(Maximum Rate*Substrate Concentration)/Michaelis ConstantOpenImg
Can the Initial Reaction Rate at Low Substrate Concentration be negative?
Yes, the Initial Reaction Rate at Low Substrate Concentration, measured in Reaction Rate can be negative.
Which unit is used to measure Initial Reaction Rate at Low Substrate Concentration?
Initial Reaction Rate at Low Substrate Concentration is usually measured using the Mole per Liter Second[mol/L*s] for Reaction Rate. Millimole per Liter Second[mol/L*s], Mole per Cubic Meter Second[mol/L*s] are the few other units in which Initial Reaction Rate at Low Substrate Concentration can be measured.
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