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Initial Enzyme in Competitive Inhibition given Enzyme Substrate Complex Concentration Formula

GoInitial Enzyme Concentration of Competitive Inhibition of Enzyme Catalysis Formula

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The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction. Check FAQs

[E 0] = \frac{ES \cdot (K M \cdot (1 + (\frac{I}{K i})) + S)}{S}

[E₀] - Initial Enzyme Concentration?ES - Enzyme Substrate Complex Concentration?K_M - Michaelis Constant?I - Inhibitor Concentration?K_i - Enzyme Inhibitor Dissociation Constant?S - Substrate Concentration?

Initial Enzyme in Competitive Inhibition given Enzyme Substrate Complex Concentration Example

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Here is how the Initial Enzyme in Competitive Inhibition given Enzyme Substrate Complex Concentration equation looks like with Values.

Here is how the Initial Enzyme in Competitive Inhibition given Enzyme Substrate Complex Concentration equation looks like with Units.

Here is how the Initial Enzyme in Competitive Inhibition given Enzyme Substrate Complex Concentration equation looks like.

39.4737 = \frac{10 \cdot (3 \cdot (1 + (\frac{9}{19})) + 1.5)}{1.5}

39.4737mol/L = \frac{10mol/L \cdot (3mol/L \cdot (1 + (\frac{9mol/L}{19mol/L})) + 1.5mol/L)}{1.5mol/L}

39.4737 = \frac{10 \cdot (3 \cdot (1 + (\frac{9}{19})) + 1.5)}{1.5}

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Initial Enzyme in Competitive Inhibition given Enzyme Substrate Complex Concentration Solution

Follow our step by step solution on how to calculate Initial Enzyme in Competitive Inhibition given Enzyme Substrate Complex Concentration?

FIRST Step Consider the formula

[E 0] = \frac{ES \cdot (K M \cdot (1 + (\frac{I}{K i})) + S)}{S}

Next Step Substitute values of Variables

∴

[E 0] = \frac{10mol/L \cdot (3mol/L \cdot (1 + (\frac{9mol/L}{19mol/L})) + 1.5mol/L)}{1.5mol/L}

Next Step Convert Units

∴

[E 0] = \frac{10000mol/m³ \cdot (3000mol/m³ \cdot (1 + (\frac{9000mol/m³}{19000mol/m³})) + 1500mol/m³)}{1500mol/m³}

Next Step Prepare to Evaluate

∴

[E 0] = \frac{10000 \cdot (3000 \cdot (1 + (\frac{9000}{19000})) + 1500)}{1500}

Next Step Evaluate

∴

[E 0] = 39473.6842105263mol/m³

Next Step Convert to Output's Unit

∴

[E 0] = 39.4736842105263mol/L

LAST Step Rounding Answer

∴

[E 0] = 39.4737mol/L

Initial Enzyme in Competitive Inhibition given Enzyme Substrate Complex Concentration Formula Elements

Variables

Initial Enzyme Concentration

The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction.

Symbol: [E₀]

Measurement: Molar ConcentrationUnit: mol/L

Note: Value should be greater than 0.

Formulas to find Initial Enzyme Concentration

Enzyme Substrate Complex Concentration

The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate.

Symbol: ES

Measurement: Molar ConcentrationUnit: mol/L

Note: Value can be positive or negative.

Formulas to find Enzyme Substrate Complex Concentration

Michaelis Constant

The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.

Symbol: K_M

Measurement: Molar ConcentrationUnit: mol/L

Note: Value can be positive or negative.

Formulas to find Michaelis Constant

Inhibitor Concentration

The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system.

Symbol: I

Measurement: Molar ConcentrationUnit: mol/L

Note: Value can be positive or negative.

Formulas to find Inhibitor Concentration

Enzyme Inhibitor Dissociation Constant

The Enzyme Inhibitor Dissociation Constant is measured by the method in which the inhibitor is titrated into a solution of enzyme and the heat released or absorbed is measured.

Symbol: K_i

Measurement: Molar ConcentrationUnit: mol/L

Note: Value can be positive or negative.

Formulas to find Enzyme Inhibitor Dissociation Constant

Substrate Concentration

The Substrate Concentration is the number of moles of substrate per liter solution.

Symbol: S

Measurement: Molar ConcentrationUnit: mol/L

Note: Value can be positive or negative.

Formulas to find Substrate Concentration

Other Formulas to find Initial Enzyme Concentration

Go Initial Enzyme Concentration of Competitive Inhibition of Enzyme Catalysis

[E 0] = \frac{V 0 \cdot (K M \cdot (1 + (\frac{I}{K i})) + S)}{k 2 \cdot S}

Other formulas in Competitive Inhibitor category

Go Apparent Value of Michaelis Menten Constant in Presence of Competitive Inhibition

Km app = \frac{S \cdot (V max - V 0)}{V 0}

Go Substrate Concentration of Competitive Inhibition of Enzyme Catalysis

S = \frac{V 0 \cdot (K M \cdot (1 + (\frac{I}{K i})))}{(k 2 \cdot [E 0]) - V 0}

Go Substrate Concentration in Competitive Inhibition given Maximum Rate of System

S = \frac{V 0 \cdot (K M \cdot (1 + (\frac{I}{K i})))}{V max - V 0}

Go Substrate Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration

S = \frac{ES \cdot (K M \cdot (1 + (\frac{I}{K i})))}{([E 0]) - ES}

How to Evaluate Initial Enzyme in Competitive Inhibition given Enzyme Substrate Complex Concentration?

Initial Enzyme in Competitive Inhibition given Enzyme Substrate Complex Concentration evaluator uses Initial Enzyme Concentration = (Enzyme Substrate Complex Concentration*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))+Substrate Concentration))/(Substrate Concentration) to evaluate the Initial Enzyme Concentration, The Initial enzyme in competitive inhibition given enzyme substrate complex concentration formula is defined as a plot of the reaction velocity (V0) associated with the concentration [S] of the substrate can then be used to determine values such as Vmax, initial velocity, and Km. Initial Enzyme Concentration is denoted by [E₀] symbol.

How to evaluate Initial Enzyme in Competitive Inhibition given Enzyme Substrate Complex Concentration using this online evaluator? To use this online evaluator for Initial Enzyme in Competitive Inhibition given Enzyme Substrate Complex Concentration, enter Enzyme Substrate Complex Concentration (ES), Michaelis Constant (K_M), Inhibitor Concentration (I), Enzyme Inhibitor Dissociation Constant (K_i) & Substrate Concentration (S) and hit the calculate button.

FAQs on Initial Enzyme in Competitive Inhibition given Enzyme Substrate Complex Concentration

What is the formula to find Initial Enzyme in Competitive Inhibition given Enzyme Substrate Complex Concentration?

The formula of Initial Enzyme in Competitive Inhibition given Enzyme Substrate Complex Concentration is expressed as Initial Enzyme Concentration = (Enzyme Substrate Complex Concentration*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))+Substrate Concentration))/(Substrate Concentration). Here is an example- 0.039474 = (10000*(3000*(1+(9000/19000))+1500))/(1500).

How to calculate Initial Enzyme in Competitive Inhibition given Enzyme Substrate Complex Concentration?

With Enzyme Substrate Complex Concentration (ES), Michaelis Constant (K_M), Inhibitor Concentration (I), Enzyme Inhibitor Dissociation Constant (K_i) & Substrate Concentration (S) we can find Initial Enzyme in Competitive Inhibition given Enzyme Substrate Complex Concentration using the formula - Initial Enzyme Concentration = (Enzyme Substrate Complex Concentration*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))+Substrate Concentration))/(Substrate Concentration).

What are the other ways to Calculate Initial Enzyme Concentration?

Here are the different ways to Calculate Initial Enzyme Concentration-

Initial Enzyme Concentration=(Initial Reaction Rate*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))+Substrate Concentration))/(Final Rate Constant*Substrate Concentration)

Can the Initial Enzyme in Competitive Inhibition given Enzyme Substrate Complex Concentration be negative?

No, the Initial Enzyme in Competitive Inhibition given Enzyme Substrate Complex Concentration, measured in Molar Concentration cannot be negative.

Which unit is used to measure Initial Enzyme in Competitive Inhibition given Enzyme Substrate Complex Concentration?

Initial Enzyme in Competitive Inhibition given Enzyme Substrate Complex Concentration is usually measured using the Mole per Liter[mol/L] for Molar Concentration. Mole per Cubic Meter[mol/L], Mole per Cubic Millimeter[mol/L], Kilomole per Cubic Meter[mol/L] are the few other units in which Initial Enzyme in Competitive Inhibition given Enzyme Substrate Complex Concentration can be measured.

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Initial Enzyme in Competitive Inhibition given Enzyme Substrate Complex Concentration Formula

​GoInitial Enzyme Concentration of Competitive Inhibition of Enzyme Catalysis Formula

Initial Enzyme in Competitive Inhibition given Enzyme Substrate Complex Concentration Example

Initial Enzyme in Competitive Inhibition given Enzyme Substrate Complex Concentration Solution

Initial Enzyme in Competitive Inhibition given Enzyme Substrate Complex Concentration Formula Elements

Other Formulas to find Initial Enzyme Concentration

Other formulas in Competitive Inhibitor category

How to Evaluate Initial Enzyme in Competitive Inhibition given Enzyme Substrate Complex Concentration?

FAQs on Initial Enzyme in Competitive Inhibition given Enzyme Substrate Complex Concentration

Variable Name

GoInitial Enzyme Concentration of Competitive Inhibition of Enzyme Catalysis Formula