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The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction. Check FAQs
[E0]=V0(KM(1+(IKi))+S)k2S
[E0] - Initial Enzyme Concentration?V0 - Initial Reaction Rate?KM - Michaelis Constant?I - Inhibitor Concentration?Ki - Enzyme Inhibitor Dissociation Constant?S - Substrate Concentration?k2 - Final Rate Constant?

Initial Enzyme Concentration of Competitive Inhibition of Enzyme Catalysis Example

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Here is how the Initial Enzyme Concentration of Competitive Inhibition of Enzyme Catalysis equation looks like with Values.

Here is how the Initial Enzyme Concentration of Competitive Inhibition of Enzyme Catalysis equation looks like with Units.

Here is how the Initial Enzyme Concentration of Competitive Inhibition of Enzyme Catalysis equation looks like.

0.0772Edit=0.45Edit(3Edit(1+(9Edit19Edit))+1.5Edit)23Edit1.5Edit
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Initial Enzyme Concentration of Competitive Inhibition of Enzyme Catalysis Solution

Follow our step by step solution on how to calculate Initial Enzyme Concentration of Competitive Inhibition of Enzyme Catalysis?

FIRST Step Consider the formula
[E0]=V0(KM(1+(IKi))+S)k2S
Next Step Substitute values of Variables
[E0]=0.45mol/L*s(3mol/L(1+(9mol/L19mol/L))+1.5mol/L)23s⁻¹1.5mol/L
Next Step Convert Units
[E0]=450mol/m³*s(3000mol/m³(1+(9000mol/m³19000mol/m³))+1500mol/m³)23s⁻¹1500mol/m³
Next Step Prepare to Evaluate
[E0]=450(3000(1+(900019000))+1500)231500
Next Step Evaluate
[E0]=77.2311212814645mol/m³
Next Step Convert to Output's Unit
[E0]=0.0772311212814645mol/L
LAST Step Rounding Answer
[E0]=0.0772mol/L

Initial Enzyme Concentration of Competitive Inhibition of Enzyme Catalysis Formula Elements

Variables
Initial Enzyme Concentration
The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction.
Symbol: [E0]
Measurement: Molar ConcentrationUnit: mol/L
Note: Value should be greater than 0.
Initial Reaction Rate
The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place.
Symbol: V0
Measurement: Reaction RateUnit: mol/L*s
Note: Value can be positive or negative.
Michaelis Constant
The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.
Symbol: KM
Measurement: Molar ConcentrationUnit: mol/L
Note: Value can be positive or negative.
Inhibitor Concentration
The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system.
Symbol: I
Measurement: Molar ConcentrationUnit: mol/L
Note: Value can be positive or negative.
Enzyme Inhibitor Dissociation Constant
The Enzyme Inhibitor Dissociation Constant is measured by the method in which the inhibitor is titrated into a solution of enzyme and the heat released or absorbed is measured.
Symbol: Ki
Measurement: Molar ConcentrationUnit: mol/L
Note: Value can be positive or negative.
Substrate Concentration
The Substrate Concentration is the number of moles of substrate per liter solution.
Symbol: S
Measurement: Molar ConcentrationUnit: mol/L
Note: Value can be positive or negative.
Final Rate Constant
The Final Rate Constant is the rate constant when the enzyme-substrate complex on reaction with inhibitor is converted into the enzyme catalyst and product.
Symbol: k2
Measurement: First Order Reaction Rate ConstantUnit: s⁻¹
Note: Value can be positive or negative.

Other Formulas to find Initial Enzyme Concentration

​Go Initial Enzyme in Competitive Inhibition given Enzyme Substrate Complex Concentration
[E0]=ES(KM(1+(IKi))+S)S

Other formulas in Competitive Inhibitor category

​Go Apparent Value of Michaelis Menten Constant in Presence of Competitive Inhibition
Kmapp=S(Vmax-V0)V0
​Go Substrate Concentration of Competitive Inhibition of Enzyme Catalysis
S=V0(KM(1+(IKi)))(k2[E0])-V0

How to Evaluate Initial Enzyme Concentration of Competitive Inhibition of Enzyme Catalysis?

Initial Enzyme Concentration of Competitive Inhibition of Enzyme Catalysis evaluator uses Initial Enzyme Concentration = (Initial Reaction Rate*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))+Substrate Concentration))/(Final Rate Constant*Substrate Concentration) to evaluate the Initial Enzyme Concentration, The Initial enzyme concentration of competitive inhibition of enzyme catalysis formula is defined as a plot of the reaction velocity (V0) associated with the concentration [S] of the substrate can then be used to determine values such as Vmax, initial velocity, and Km. Initial Enzyme Concentration is denoted by [E0] symbol.

How to evaluate Initial Enzyme Concentration of Competitive Inhibition of Enzyme Catalysis using this online evaluator? To use this online evaluator for Initial Enzyme Concentration of Competitive Inhibition of Enzyme Catalysis, enter Initial Reaction Rate (V0), Michaelis Constant (KM), Inhibitor Concentration (I), Enzyme Inhibitor Dissociation Constant (Ki), Substrate Concentration (S) & Final Rate Constant (k2) and hit the calculate button.

FAQs on Initial Enzyme Concentration of Competitive Inhibition of Enzyme Catalysis

What is the formula to find Initial Enzyme Concentration of Competitive Inhibition of Enzyme Catalysis?
The formula of Initial Enzyme Concentration of Competitive Inhibition of Enzyme Catalysis is expressed as Initial Enzyme Concentration = (Initial Reaction Rate*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))+Substrate Concentration))/(Final Rate Constant*Substrate Concentration). Here is an example- 7.7E-5 = (450*(3000*(1+(9000/19000))+1500))/(23*1500).
How to calculate Initial Enzyme Concentration of Competitive Inhibition of Enzyme Catalysis?
With Initial Reaction Rate (V0), Michaelis Constant (KM), Inhibitor Concentration (I), Enzyme Inhibitor Dissociation Constant (Ki), Substrate Concentration (S) & Final Rate Constant (k2) we can find Initial Enzyme Concentration of Competitive Inhibition of Enzyme Catalysis using the formula - Initial Enzyme Concentration = (Initial Reaction Rate*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))+Substrate Concentration))/(Final Rate Constant*Substrate Concentration).
What are the other ways to Calculate Initial Enzyme Concentration?
Here are the different ways to Calculate Initial Enzyme Concentration-
  • Initial Enzyme Concentration=(Enzyme Substrate Complex Concentration*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))+Substrate Concentration))/(Substrate Concentration)OpenImg
Can the Initial Enzyme Concentration of Competitive Inhibition of Enzyme Catalysis be negative?
No, the Initial Enzyme Concentration of Competitive Inhibition of Enzyme Catalysis, measured in Molar Concentration cannot be negative.
Which unit is used to measure Initial Enzyme Concentration of Competitive Inhibition of Enzyme Catalysis?
Initial Enzyme Concentration of Competitive Inhibition of Enzyme Catalysis is usually measured using the Mole per Liter[mol/L] for Molar Concentration. Mole per Cubic Meter[mol/L], Mole per Cubic Millimeter[mol/L], Kilomole per Cubic Meter[mol/L] are the few other units in which Initial Enzyme Concentration of Competitive Inhibition of Enzyme Catalysis can be measured.
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