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Initial Enzyme Concentration in Enzymatic Reaction Mechanism Formula

GoInitial Enzyme Concentration at Low Substrate Concentration Formula

GoInitial Enzyme Concentration given Catalytic Rate Constant and Dissociation Rate Constants Formula

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The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction. Check FAQs

[E 0] = (\frac{k r \cdot ES}{k f \cdot S}) + ES

[E₀] - Initial Enzyme Concentration?k_r - Reverse Rate Constant?ES - Enzyme Substrate Complex Concentration?k_f - Forward Rate Constant?S - Substrate Concentration?

Initial Enzyme Concentration in Enzymatic Reaction Mechanism Example

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Here is how the Initial Enzyme Concentration in Enzymatic Reaction Mechanism equation looks like with Values.

Here is how the Initial Enzyme Concentration in Enzymatic Reaction Mechanism equation looks like with Units.

Here is how the Initial Enzyme Concentration in Enzymatic Reaction Mechanism equation looks like.

29.3237 = (\frac{20 \cdot 10}{6.9 \cdot 1.5}) + 10

29.3237mol/L = (\frac{20mol/L*s \cdot 10mol/L}{6.9s⁻¹ \cdot 1.5mol/L}) + 10mol/L

29.3237 = (\frac{20 \cdot 10}{6.9 \cdot 1.5}) + 10

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Initial Enzyme Concentration in Enzymatic Reaction Mechanism Solution

Follow our step by step solution on how to calculate Initial Enzyme Concentration in Enzymatic Reaction Mechanism?

FIRST Step Consider the formula

[E 0] = (\frac{k r \cdot ES}{k f \cdot S}) + ES

Next Step Substitute values of Variables

∴

[E 0] = (\frac{20mol/L*s \cdot 10mol/L}{6.9s⁻¹ \cdot 1.5mol/L}) + 10mol/L

Next Step Convert Units

∴

[E 0] = (\frac{20000mol/m³*s \cdot 10000mol/m³}{6.9s⁻¹ \cdot 1500mol/m³}) + 10000mol/m³

Next Step Prepare to Evaluate

∴

[E 0] = (\frac{20000 \cdot 10000}{6.9 \cdot 1500}) + 10000

Next Step Evaluate

∴

[E 0] = 29323.6714975845mol/m³

Next Step Convert to Output's Unit

∴

[E 0] = 29.3236714975845mol/L

LAST Step Rounding Answer

∴

[E 0] = 29.3237mol/L

Initial Enzyme Concentration in Enzymatic Reaction Mechanism Formula Elements

Variables

Initial Enzyme Concentration

The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction.

Symbol: [E₀]

Measurement: Molar ConcentrationUnit: mol/L

Note: Value should be greater than 0.

Formulas to find Initial Enzyme Concentration

Reverse Rate Constant

The Reverse Rate Constant is defined as the rate constant for the backward reaction.

Symbol: k_r

Measurement: Reaction RateUnit: mol/L*s

Note: Value can be positive or negative.

Formulas to find Reverse Rate Constant

Enzyme Substrate Complex Concentration

The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate.

Symbol: ES

Measurement: Molar ConcentrationUnit: mol/L

Note: Value can be positive or negative.

Formulas to find Enzyme Substrate Complex Concentration

Forward Rate Constant

The Forward Rate Constant is defined as the rate constant for the forward occurring reaction.

Symbol: k_f

Measurement: First Order Reaction Rate ConstantUnit: s⁻¹

Note: Value can be positive or negative.

Formulas to find Forward Rate Constant

Substrate Concentration

The Substrate Concentration is the number of moles of substrate per liter solution.

Symbol: S

Measurement: Molar ConcentrationUnit: mol/L

Note: Value can be positive or negative.

Formulas to find Substrate Concentration

Other Formulas to find Initial Enzyme Concentration

Go Initial Enzyme Concentration at Low Substrate Concentration

[E 0] = \frac{V 0 \cdot K M}{k cat \cdot S}

Go Initial Enzyme Concentration given Catalytic Rate Constant and Dissociation Rate Constants

[E 0] = \frac{V 0 \cdot (K D + S)}{k cat \cdot S}

Go Initial Enzyme Concentration given Rate Constant and Maximum Rate

[E 0] = \frac{V max}{k 2}

Other formulas in Complex Concentration category

Go Substrate Concentration given Catalytic Rate Constant and Initial Enzyme Concentration

S o = \frac{K M \cdot V 0}{(k cat \cdot [E 0]) - V 0}

Go Substrate Concentration if Michaelis Constant is very Large than Substrate Concentration

S = \frac{V 0 \cdot K M}{k cat \cdot [E 0]}

Go Substrate Concentration given Maximum Rate at Low Concentration

S = \frac{V 0 \cdot K M}{V max}

Go Enzyme Substrate Complex Concentration in Instantaneous Chemical Equilibrium

ES = \frac{k f \cdot [E 0] \cdot S}{k r + (k f \cdot S)}

How to Evaluate Initial Enzyme Concentration in Enzymatic Reaction Mechanism?

Initial Enzyme Concentration in Enzymatic Reaction Mechanism evaluator uses Initial Enzyme Concentration = ((Reverse Rate Constant*Enzyme Substrate Complex Concentration)/(Forward Rate Constant*Substrate Concentration))+Enzyme Substrate Complex Concentration to evaluate the Initial Enzyme Concentration, The Initial enzyme concentration in enzymatic reaction mechanism formula is defined as the relation with the rate constant of forward and backward reaction and with the concentration of enzyme and substrate. Initial Enzyme Concentration is denoted by [E₀] symbol.

How to evaluate Initial Enzyme Concentration in Enzymatic Reaction Mechanism using this online evaluator? To use this online evaluator for Initial Enzyme Concentration in Enzymatic Reaction Mechanism, enter Reverse Rate Constant (k_r), Enzyme Substrate Complex Concentration (ES), Forward Rate Constant (k_f) & Substrate Concentration (S) and hit the calculate button.

FAQs on Initial Enzyme Concentration in Enzymatic Reaction Mechanism

What is the formula to find Initial Enzyme Concentration in Enzymatic Reaction Mechanism?

The formula of Initial Enzyme Concentration in Enzymatic Reaction Mechanism is expressed as Initial Enzyme Concentration = ((Reverse Rate Constant*Enzyme Substrate Complex Concentration)/(Forward Rate Constant*Substrate Concentration))+Enzyme Substrate Complex Concentration. Here is an example- 0.029324 = ((20000*10000)/(6.9*1500))+10000.

How to calculate Initial Enzyme Concentration in Enzymatic Reaction Mechanism?

With Reverse Rate Constant (k_r), Enzyme Substrate Complex Concentration (ES), Forward Rate Constant (k_f) & Substrate Concentration (S) we can find Initial Enzyme Concentration in Enzymatic Reaction Mechanism using the formula - Initial Enzyme Concentration = ((Reverse Rate Constant*Enzyme Substrate Complex Concentration)/(Forward Rate Constant*Substrate Concentration))+Enzyme Substrate Complex Concentration.

What are the other ways to Calculate Initial Enzyme Concentration?

Here are the different ways to Calculate Initial Enzyme Concentration-

Initial Enzyme Concentration=(Initial Reaction Rate*Michaelis Constant)/(Catalytic Rate Constant*Substrate Concentration)
Initial Enzyme Concentration=(Initial Reaction Rate*(Dissociation Rate Constant+Substrate Concentration))/(Catalytic Rate Constant*Substrate Concentration)
Initial Enzyme Concentration=Maximum Rate/Final Rate Constant

Can the Initial Enzyme Concentration in Enzymatic Reaction Mechanism be negative?

No, the Initial Enzyme Concentration in Enzymatic Reaction Mechanism, measured in Molar Concentration cannot be negative.

Which unit is used to measure Initial Enzyme Concentration in Enzymatic Reaction Mechanism?

Initial Enzyme Concentration in Enzymatic Reaction Mechanism is usually measured using the Mole per Liter[mol/L] for Molar Concentration. Mole per Cubic Meter[mol/L], Mole per Cubic Millimeter[mol/L], Kilomole per Cubic Meter[mol/L] are the few other units in which Initial Enzyme Concentration in Enzymatic Reaction Mechanism can be measured.

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Initial Enzyme Concentration in Enzymatic Reaction Mechanism Formula

​GoInitial Enzyme Concentration at Low Substrate Concentration Formula

​GoInitial Enzyme Concentration given Catalytic Rate Constant and Dissociation Rate Constants Formula

Initial Enzyme Concentration in Enzymatic Reaction Mechanism Example

Initial Enzyme Concentration in Enzymatic Reaction Mechanism Solution

Initial Enzyme Concentration in Enzymatic Reaction Mechanism Formula Elements

Other Formulas to find Initial Enzyme Concentration

Other formulas in Complex Concentration category

How to Evaluate Initial Enzyme Concentration in Enzymatic Reaction Mechanism?

FAQs on Initial Enzyme Concentration in Enzymatic Reaction Mechanism

Variable Name

GoInitial Enzyme Concentration at Low Substrate Concentration Formula

GoInitial Enzyme Concentration given Catalytic Rate Constant and Dissociation Rate Constants Formula