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Enzyme Concentration Initially is defined as the concentration of enzyme at the start of the reaction. Check FAQs
[Einitial]=Vmaxkcat
[Einitial] - Enzyme Concentration Initially?Vmax - Maximum Rate?kcat - Catalytic Rate Constant?

Initial Enzyme Concentration if Substrate Concentration is Higher than Michaelis Constant Example

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Here is how the Initial Enzyme Concentration if Substrate Concentration is Higher than Michaelis Constant equation looks like with Values.

Here is how the Initial Enzyme Concentration if Substrate Concentration is Higher than Michaelis Constant equation looks like with Units.

Here is how the Initial Enzyme Concentration if Substrate Concentration is Higher than Michaelis Constant equation looks like.

61.5385Edit=40Edit0.65Edit
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Initial Enzyme Concentration if Substrate Concentration is Higher than Michaelis Constant Solution

Follow our step by step solution on how to calculate Initial Enzyme Concentration if Substrate Concentration is Higher than Michaelis Constant?

FIRST Step Consider the formula
[Einitial]=Vmaxkcat
Next Step Substitute values of Variables
[Einitial]=40mol/L*s0.65s⁻¹
Next Step Convert Units
[Einitial]=40000mol/m³*s0.65s⁻¹
Next Step Prepare to Evaluate
[Einitial]=400000.65
Next Step Evaluate
[Einitial]=61538.4615384615mol/m³
Next Step Convert to Output's Unit
[Einitial]=61.5384615384615mol/L
LAST Step Rounding Answer
[Einitial]=61.5385mol/L

Initial Enzyme Concentration if Substrate Concentration is Higher than Michaelis Constant Formula Elements

Variables
Enzyme Concentration Initially
Enzyme Concentration Initially is defined as the concentration of enzyme at the start of the reaction.
Symbol: [Einitial]
Measurement: Molar ConcentrationUnit: mol/L
Note: Value can be positive or negative.
Maximum Rate
The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration.
Symbol: Vmax
Measurement: Reaction RateUnit: mol/L*s
Note: Value can be positive or negative.
Catalytic Rate Constant
The Catalytic Rate Constant is defined as the rate constant for conversion of the enzyme-substrate complex to enzyme and product.
Symbol: kcat
Measurement: First Order Reaction Rate ConstantUnit: s⁻¹
Note: Value can be positive or negative.

Other Formulas to find Enzyme Concentration Initially

​Go Initial Enzyme Concentration given Dissociation Rate Constant
[Einitial]=ES(KD+S)S
​Go Initial Concentration of Enzyme in presence of Inhibitor by Enzyme Conservation Law
[Einitial]=(E+ES+EI)

Other formulas in Michaelis Menten Kinetics Equation category

​Go Modifying Factor of Enzyme Substrate Complex
α'=1+(IKi')
​Go Initial Rate of System given Rate Constant and Enzyme Substrate Complex Concentration
VRC=k2ES
​Go Initial Reaction Rate given Dissociation Rate Constant
VDRC=VmaxSKD+S
​Go Maximum Rate given Dissociation Rate Constant
Vmax_DRC=V0(KD+S)S

How to Evaluate Initial Enzyme Concentration if Substrate Concentration is Higher than Michaelis Constant?

Initial Enzyme Concentration if Substrate Concentration is Higher than Michaelis Constant evaluator uses Enzyme Concentration Initially = Maximum Rate/Catalytic Rate Constant to evaluate the Enzyme Concentration Initially, The Initial enzyme concentration if substrate concentration is higher than Michaelis constant formula is defined as the ratio of the maximum rate to the catalytic rate constant. Here substrate concentration is very large in comparison to Michaelis constant. Enzyme Concentration Initially is denoted by [Einitial] symbol.

How to evaluate Initial Enzyme Concentration if Substrate Concentration is Higher than Michaelis Constant using this online evaluator? To use this online evaluator for Initial Enzyme Concentration if Substrate Concentration is Higher than Michaelis Constant, enter Maximum Rate (Vmax) & Catalytic Rate Constant (kcat) and hit the calculate button.

FAQs on Initial Enzyme Concentration if Substrate Concentration is Higher than Michaelis Constant

What is the formula to find Initial Enzyme Concentration if Substrate Concentration is Higher than Michaelis Constant?
The formula of Initial Enzyme Concentration if Substrate Concentration is Higher than Michaelis Constant is expressed as Enzyme Concentration Initially = Maximum Rate/Catalytic Rate Constant. Here is an example- 0.061538 = 40000/0.65.
How to calculate Initial Enzyme Concentration if Substrate Concentration is Higher than Michaelis Constant?
With Maximum Rate (Vmax) & Catalytic Rate Constant (kcat) we can find Initial Enzyme Concentration if Substrate Concentration is Higher than Michaelis Constant using the formula - Enzyme Concentration Initially = Maximum Rate/Catalytic Rate Constant.
What are the other ways to Calculate Enzyme Concentration Initially?
Here are the different ways to Calculate Enzyme Concentration Initially-
  • Enzyme Concentration Initially=(Enzyme Substrate Complex Concentration*(Dissociation Rate Constant+Substrate Concentration))/(Substrate Concentration)OpenImg
  • Enzyme Concentration Initially=(Catalyst Concentration+Enzyme Substrate Complex Concentration+Enzyme Inhibitor Complex Concentration)OpenImg
Can the Initial Enzyme Concentration if Substrate Concentration is Higher than Michaelis Constant be negative?
Yes, the Initial Enzyme Concentration if Substrate Concentration is Higher than Michaelis Constant, measured in Molar Concentration can be negative.
Which unit is used to measure Initial Enzyme Concentration if Substrate Concentration is Higher than Michaelis Constant?
Initial Enzyme Concentration if Substrate Concentration is Higher than Michaelis Constant is usually measured using the Mole per Liter[mol/L] for Molar Concentration. Mole per Cubic Meter[mol/L], Mole per Cubic Millimeter[mol/L], Kilomole per Cubic Meter[mol/L] are the few other units in which Initial Enzyme Concentration if Substrate Concentration is Higher than Michaelis Constant can be measured.
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