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Inhibitor's Dissociation Constant given Michaelis Menten Constant Formula

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The Enzyme Inhibitor Dissociation Constant is measured by the method in which the inhibitor is titrated into a solution of enzyme and the heat released or absorbed is measured. Check FAQs

K i = (\frac{I}{(\frac{Km app}{K M}) - 1})

K_i - Enzyme Inhibitor Dissociation Constant?I - Inhibitor Concentration?Km^app - Apparent Michaelis Constant?K_M - Michaelis Constant?

Inhibitor's Dissociation Constant given Michaelis Menten Constant Example

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Here is how the Inhibitor's Dissociation Constant given Michaelis Menten Constant equation looks like with Values.

Here is how the Inhibitor's Dissociation Constant given Michaelis Menten Constant equation looks like with Units.

Here is how the Inhibitor's Dissociation Constant given Michaelis Menten Constant equation looks like.

3 = (\frac{9}{(\frac{12}{3}) - 1})

3mol/L = (\frac{9mol/L}{(\frac{12mol/L}{3mol/L}) - 1})

3 = (\frac{9}{(\frac{12}{3}) - 1})

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Inhibitor's Dissociation Constant given Michaelis Menten Constant Solution

Follow our step by step solution on how to calculate Inhibitor's Dissociation Constant given Michaelis Menten Constant?

FIRST Step Consider the formula

K i = (\frac{I}{(\frac{Km app}{K M}) - 1})

Next Step Substitute values of Variables

∴

K i = (\frac{9mol/L}{(\frac{12mol/L}{3mol/L}) - 1})

Next Step Convert Units

∴

K i = (\frac{9000mol/m³}{(\frac{12000mol/m³}{3000mol/m³}) - 1})

Next Step Prepare to Evaluate

∴

K i = (\frac{9000}{(\frac{12000}{3000}) - 1})

Next Step Evaluate

∴

K i = 3000mol/m³

LAST Step Convert to Output's Unit

∴

K i = 3mol/L

Inhibitor's Dissociation Constant given Michaelis Menten Constant Formula Elements

Variables

Enzyme Inhibitor Dissociation Constant

The Enzyme Inhibitor Dissociation Constant is measured by the method in which the inhibitor is titrated into a solution of enzyme and the heat released or absorbed is measured.

Symbol: K_i

Measurement: Molar ConcentrationUnit: mol/L

Note: Value can be positive or negative.

Formulas to find Enzyme Inhibitor Dissociation Constant

Inhibitor Concentration

The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system.

Symbol: I

Measurement: Molar ConcentrationUnit: mol/L

Note: Value can be positive or negative.

Formulas to find Inhibitor Concentration

Apparent Michaelis Constant

The Apparent Michaelis Constant is defined as the Michaelis–Menten constant in the presence of a competitive inhibitor.

Symbol: Km^app

Measurement: Molar ConcentrationUnit: mol/L

Note: Value can be positive or negative.

Formulas to find Apparent Michaelis Constant

Michaelis Constant

The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.

Symbol: K_M

Measurement: Molar ConcentrationUnit: mol/L

Note: Value can be positive or negative.

Formulas to find Michaelis Constant

Other formulas in Michaelis Menten Kinetics Equation category

Go Maximum Rate of System from Michaelis Menten Kinetics equation

V max = \frac{V 0 \cdot (K M + S)}{S}

Go Substrate Concentration from Michaelis Menten Kinetics Equation

S = \frac{K M \cdot V 0}{V max - V 0}

Go Michaelis Constant from Michaelis Menten kinetics equation

K M = S \cdot (\frac{V max - V 0}{V 0})

Go Michaelis Constant given Catalytic Rate Constant and Initial Enzyme Concentration

K M = \frac{S \cdot ((k cat \cdot [E 0]) - V 0)}{V 0}

How to Evaluate Inhibitor's Dissociation Constant given Michaelis Menten Constant?

Inhibitor's Dissociation Constant given Michaelis Menten Constant evaluator uses Enzyme Inhibitor Dissociation Constant = (Inhibitor Concentration/((Apparent Michaelis Constant/Michaelis Constant)-1)) to evaluate the Enzyme Inhibitor Dissociation Constant, The Inhibitor's dissociation constant given Michaelis Menten constant formula is defined as the relation with Michaelis constant and apparent Michaelis constant and with the inhibitor concentration. Enzyme Inhibitor Dissociation Constant is denoted by K_i symbol.

How to evaluate Inhibitor's Dissociation Constant given Michaelis Menten Constant using this online evaluator? To use this online evaluator for Inhibitor's Dissociation Constant given Michaelis Menten Constant, enter Inhibitor Concentration (I), Apparent Michaelis Constant (Km^app) & Michaelis Constant (K_M) and hit the calculate button.

FAQs on Inhibitor's Dissociation Constant given Michaelis Menten Constant

What is the formula to find Inhibitor's Dissociation Constant given Michaelis Menten Constant?

The formula of Inhibitor's Dissociation Constant given Michaelis Menten Constant is expressed as Enzyme Inhibitor Dissociation Constant = (Inhibitor Concentration/((Apparent Michaelis Constant/Michaelis Constant)-1)). Here is an example- 0.003 = (9000/((12000/3000)-1)).

How to calculate Inhibitor's Dissociation Constant given Michaelis Menten Constant?

With Inhibitor Concentration (I), Apparent Michaelis Constant (Km^app) & Michaelis Constant (K_M) we can find Inhibitor's Dissociation Constant given Michaelis Menten Constant using the formula - Enzyme Inhibitor Dissociation Constant = (Inhibitor Concentration/((Apparent Michaelis Constant/Michaelis Constant)-1)).

Can the Inhibitor's Dissociation Constant given Michaelis Menten Constant be negative?

Yes, the Inhibitor's Dissociation Constant given Michaelis Menten Constant, measured in Molar Concentration can be negative.

Which unit is used to measure Inhibitor's Dissociation Constant given Michaelis Menten Constant?

Inhibitor's Dissociation Constant given Michaelis Menten Constant is usually measured using the Mole per Liter[mol/L] for Molar Concentration. Mole per Cubic Meter[mol/L], Mole per Cubic Millimeter[mol/L], Kilomole per Cubic Meter[mol/L] are the few other units in which Inhibitor's Dissociation Constant given Michaelis Menten Constant can be measured.

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Inhibitor's Dissociation Constant given Michaelis Menten Constant Formula

Inhibitor's Dissociation Constant given Michaelis Menten Constant Example

Inhibitor's Dissociation Constant given Michaelis Menten Constant Solution

Inhibitor's Dissociation Constant given Michaelis Menten Constant Formula Elements

Other formulas in Michaelis Menten Kinetics Equation category

How to Evaluate Inhibitor's Dissociation Constant given Michaelis Menten Constant?

FAQs on Inhibitor's Dissociation Constant given Michaelis Menten Constant

Variable Name