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Inhibitor Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration Formula

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The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system. Check FAQs

I = ((\frac{(\frac{[E 0] \cdot S}{ES}) - S}{K M}) - 1) \cdot K i

I - Inhibitor Concentration?[E₀] - Initial Enzyme Concentration?S - Substrate Concentration?ES - Enzyme Substrate Complex Concentration?K_M - Michaelis Constant?K_i - Enzyme Inhibitor Dissociation Constant?

Inhibitor Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration Example

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Here is how the Inhibitor Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration equation looks like with Values.

Here is how the Inhibitor Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration equation looks like with Units.

Here is how the Inhibitor Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration equation looks like.

66.5 = ((\frac{(\frac{100 \cdot 1.5}{10}) - 1.5}{3}) - 1) \cdot 19

66.5mol/L = ((\frac{(\frac{100mol/L \cdot 1.5mol/L}{10mol/L}) - 1.5mol/L}{3mol/L}) - 1) \cdot 19mol/L

66.5 = ((\frac{(\frac{100 \cdot 1.5}{10}) - 1.5}{3}) - 1) \cdot 19

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Inhibitor Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration Solution

Follow our step by step solution on how to calculate Inhibitor Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration?

FIRST Step Consider the formula

I = ((\frac{(\frac{[E 0] \cdot S}{ES}) - S}{K M}) - 1) \cdot K i

Next Step Substitute values of Variables

∴

I = ((\frac{(\frac{100mol/L \cdot 1.5mol/L}{10mol/L}) - 1.5mol/L}{3mol/L}) - 1) \cdot 19mol/L

Next Step Convert Units

∴

I = ((\frac{(\frac{100000mol/m³ \cdot 1500mol/m³}{10000mol/m³}) - 1500mol/m³}{3000mol/m³}) - 1) \cdot 19000mol/m³

Next Step Prepare to Evaluate

∴

I = ((\frac{(\frac{100000 \cdot 1500}{10000}) - 1500}{3000}) - 1) \cdot 19000

Next Step Evaluate

∴

I = 66500mol/m³

LAST Step Convert to Output's Unit

∴

I = 66.5mol/L

Inhibitor Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration Formula Elements

Variables

Inhibitor Concentration

The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system.

Symbol: I

Measurement: Molar ConcentrationUnit: mol/L

Note: Value can be positive or negative.

Formulas to find Inhibitor Concentration

Initial Enzyme Concentration

The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction.

Symbol: [E₀]

Measurement: Molar ConcentrationUnit: mol/L

Note: Value should be greater than 0.

Formulas to find Initial Enzyme Concentration

Substrate Concentration

The Substrate Concentration is the number of moles of substrate per liter solution.

Symbol: S

Measurement: Molar ConcentrationUnit: mol/L

Note: Value can be positive or negative.

Formulas to find Substrate Concentration

Enzyme Substrate Complex Concentration

The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate.

Symbol: ES

Measurement: Molar ConcentrationUnit: mol/L

Note: Value can be positive or negative.

Formulas to find Enzyme Substrate Complex Concentration

Michaelis Constant

The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.

Symbol: K_M

Measurement: Molar ConcentrationUnit: mol/L

Note: Value can be positive or negative.

Formulas to find Michaelis Constant

Enzyme Inhibitor Dissociation Constant

The Enzyme Inhibitor Dissociation Constant is measured by the method in which the inhibitor is titrated into a solution of enzyme and the heat released or absorbed is measured.

Symbol: K_i

Measurement: Molar ConcentrationUnit: mol/L

Note: Value can be positive or negative.

Formulas to find Enzyme Inhibitor Dissociation Constant

Other formulas in Competitive Inhibitor category

Go Apparent Value of Michaelis Menten Constant in Presence of Competitive Inhibition

Km app = \frac{S \cdot (V max - V 0)}{V 0}

Go Substrate Concentration of Competitive Inhibition of Enzyme Catalysis

S = \frac{V 0 \cdot (K M \cdot (1 + (\frac{I}{K i})))}{(k 2 \cdot [E 0]) - V 0}

Go Substrate Concentration in Competitive Inhibition given Maximum Rate of System

S = \frac{V 0 \cdot (K M \cdot (1 + (\frac{I}{K i})))}{V max - V 0}

Go Substrate Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration

S = \frac{ES \cdot (K M \cdot (1 + (\frac{I}{K i})))}{([E 0]) - ES}

How to Evaluate Inhibitor Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration?

Inhibitor Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration evaluator uses Inhibitor Concentration = (((((Initial Enzyme Concentration*Substrate Concentration)/Enzyme Substrate Complex Concentration)-Substrate Concentration)/Michaelis Constant)-1)*Enzyme Inhibitor Dissociation Constant to evaluate the Inhibitor Concentration, The Inhibitor concentration in competitive inhibition given enzyme substrate complex concentration formula is defined as a plot of the reaction velocity (V0) associated with the concentration [S] of the substrate can then be used to determine values such as Vmax, initial velocity, and Km. Inhibitor Concentration is denoted by I symbol.

How to evaluate Inhibitor Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration using this online evaluator? To use this online evaluator for Inhibitor Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration, enter Initial Enzyme Concentration ([E₀]), Substrate Concentration (S), Enzyme Substrate Complex Concentration (ES), Michaelis Constant (K_M) & Enzyme Inhibitor Dissociation Constant (K_i) and hit the calculate button.

FAQs on Inhibitor Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration

What is the formula to find Inhibitor Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration?

The formula of Inhibitor Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration is expressed as Inhibitor Concentration = (((((Initial Enzyme Concentration*Substrate Concentration)/Enzyme Substrate Complex Concentration)-Substrate Concentration)/Michaelis Constant)-1)*Enzyme Inhibitor Dissociation Constant. Here is an example- 0.0665 = (((((100000*1500)/10000)-1500)/3000)-1)*19000.

How to calculate Inhibitor Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration?

With Initial Enzyme Concentration ([E₀]), Substrate Concentration (S), Enzyme Substrate Complex Concentration (ES), Michaelis Constant (K_M) & Enzyme Inhibitor Dissociation Constant (K_i) we can find Inhibitor Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration using the formula - Inhibitor Concentration = (((((Initial Enzyme Concentration*Substrate Concentration)/Enzyme Substrate Complex Concentration)-Substrate Concentration)/Michaelis Constant)-1)*Enzyme Inhibitor Dissociation Constant.

Can the Inhibitor Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration be negative?

Yes, the Inhibitor Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration, measured in Molar Concentration can be negative.

Which unit is used to measure Inhibitor Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration?

Inhibitor Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration is usually measured using the Mole per Liter[mol/L] for Molar Concentration. Mole per Cubic Meter[mol/L], Mole per Cubic Millimeter[mol/L], Kilomole per Cubic Meter[mol/L] are the few other units in which Inhibitor Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration can be measured.

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Inhibitor Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration Formula

Inhibitor Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration Example

Inhibitor Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration Solution

Inhibitor Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration Formula Elements

Other formulas in Competitive Inhibitor category

How to Evaluate Inhibitor Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration?

FAQs on Inhibitor Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration

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