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The Forward Rate Constant is defined as the rate constant for the forward occurring reaction. Check FAQs
kf=krESS([E0]-ES)
kf - Forward Rate Constant?kr - Reverse Rate Constant?ES - Enzyme Substrate Complex Concentration?S - Substrate Concentration?[E0] - Initial Enzyme Concentration?

Forward Rate Constant in Enzymatic Reaction mechanism Example

With values
With units
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Here is how the Forward Rate Constant in Enzymatic Reaction mechanism equation looks like with Values.

Here is how the Forward Rate Constant in Enzymatic Reaction mechanism equation looks like with Units.

Here is how the Forward Rate Constant in Enzymatic Reaction mechanism equation looks like.

1.4815Edit=20Edit10Edit1.5Edit(100Edit-10Edit)
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Forward Rate Constant in Enzymatic Reaction mechanism Solution

Follow our step by step solution on how to calculate Forward Rate Constant in Enzymatic Reaction mechanism?

FIRST Step Consider the formula
kf=krESS([E0]-ES)
Next Step Substitute values of Variables
kf=20mol/L*s10mol/L1.5mol/L(100mol/L-10mol/L)
Next Step Convert Units
kf=20000mol/m³*s10000mol/m³1500mol/m³(100000mol/m³-10000mol/m³)
Next Step Prepare to Evaluate
kf=20000100001500(100000-10000)
Next Step Evaluate
kf=1.48148148148148s⁻¹
LAST Step Rounding Answer
kf=1.4815s⁻¹

Forward Rate Constant in Enzymatic Reaction mechanism Formula Elements

Variables
Forward Rate Constant
The Forward Rate Constant is defined as the rate constant for the forward occurring reaction.
Symbol: kf
Measurement: First Order Reaction Rate ConstantUnit: s⁻¹
Note: Value can be positive or negative.
Reverse Rate Constant
The Reverse Rate Constant is defined as the rate constant for the backward reaction.
Symbol: kr
Measurement: Reaction RateUnit: mol/L*s
Note: Value can be positive or negative.
Enzyme Substrate Complex Concentration
The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate.
Symbol: ES
Measurement: Molar ConcentrationUnit: mol/L
Note: Value can be positive or negative.
Substrate Concentration
The Substrate Concentration is the number of moles of substrate per liter solution.
Symbol: S
Measurement: Molar ConcentrationUnit: mol/L
Note: Value can be positive or negative.
Initial Enzyme Concentration
The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction.
Symbol: [E0]
Measurement: Molar ConcentrationUnit: mol/L
Note: Value should be greater than 0.

Other Formulas to find Forward Rate Constant

​Go Forward Rate Constant given Dissociation Rate Constant
kf=(krKD)
​Go Forward Rate Constant given Reverse and Catalytic Rate Constant
kf=(kr+kcat)(ESES)

Other formulas in Rate Constants of Enzymatic Reaction category

​Go Catalytic Rate Constant at Low Substrate Concentration
kcat=V0KM[E0]S
​Go Reverse Rate Constant in Enzymatic Reaction Mechanism
kr=kfS([E0]-ES)ES
​Go Dissociation Rate Constant in Enzymatic Reaction Mechanism
KD=krkf
​Go Reverse Rate Constant given Dissociation Rate Constant
kr=(KDkf)

How to Evaluate Forward Rate Constant in Enzymatic Reaction mechanism?

Forward Rate Constant in Enzymatic Reaction mechanism evaluator uses Forward Rate Constant = (Reverse Rate Constant*Enzyme Substrate Complex Concentration)/(Substrate Concentration*(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration)) to evaluate the Forward Rate Constant, The Forward rate constant in enzymatic reaction mechanism formula is defined as the relation with the rate constant of backward reaction and with the concentration of enzyme and substrate. Forward Rate Constant is denoted by kf symbol.

How to evaluate Forward Rate Constant in Enzymatic Reaction mechanism using this online evaluator? To use this online evaluator for Forward Rate Constant in Enzymatic Reaction mechanism, enter Reverse Rate Constant (kr), Enzyme Substrate Complex Concentration (ES), Substrate Concentration (S) & Initial Enzyme Concentration ([E0]) and hit the calculate button.

FAQs on Forward Rate Constant in Enzymatic Reaction mechanism

What is the formula to find Forward Rate Constant in Enzymatic Reaction mechanism?
The formula of Forward Rate Constant in Enzymatic Reaction mechanism is expressed as Forward Rate Constant = (Reverse Rate Constant*Enzyme Substrate Complex Concentration)/(Substrate Concentration*(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration)). Here is an example- 1.481481 = (20000*10000)/(1500*(100000-10000)).
How to calculate Forward Rate Constant in Enzymatic Reaction mechanism?
With Reverse Rate Constant (kr), Enzyme Substrate Complex Concentration (ES), Substrate Concentration (S) & Initial Enzyme Concentration ([E0]) we can find Forward Rate Constant in Enzymatic Reaction mechanism using the formula - Forward Rate Constant = (Reverse Rate Constant*Enzyme Substrate Complex Concentration)/(Substrate Concentration*(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration)).
What are the other ways to Calculate Forward Rate Constant?
Here are the different ways to Calculate Forward Rate Constant-
  • Forward Rate Constant=(Reverse Rate Constant/Dissociation Rate Constant)OpenImg
  • Forward Rate Constant=(Reverse Rate Constant+Catalytic Rate Constant)*(Enzyme Substrate Complex Concentration/(Catalyst Concentration*Substrate Concentration))OpenImg
Can the Forward Rate Constant in Enzymatic Reaction mechanism be negative?
Yes, the Forward Rate Constant in Enzymatic Reaction mechanism, measured in First Order Reaction Rate Constant can be negative.
Which unit is used to measure Forward Rate Constant in Enzymatic Reaction mechanism?
Forward Rate Constant in Enzymatic Reaction mechanism is usually measured using the 1 Per Second[s⁻¹] for First Order Reaction Rate Constant. 1 Per Millisecond[s⁻¹], 1 Per Day[s⁻¹], 1 Per Hour[s⁻¹] are the few other units in which Forward Rate Constant in Enzymatic Reaction mechanism can be measured.
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