Forster Critical Distance evaluator uses Forster Critical Distance = 0.0211*((Refractive Index of Medium)^(-4)*(Fluorescence Quantum Yield without FRET)*(Orientation Factor)*(Spectral Overlap Integral))^(1/6) to evaluate the Forster Critical Distance, The Forster Critical Distance formula is defined as the Förster distance of this pair of donor and acceptor, i.e. the distance at which the energy transfer efficiency is 50%. Forster Critical Distance is denoted by R0 symbol.
How to evaluate Forster Critical Distance using this online evaluator? To use this online evaluator for Forster Critical Distance, enter Refractive Index of Medium (η), Fluorescence Quantum Yield without FRET (Φd), Orientation Factor (κ2) & Spectral Overlap Integral (J) and hit the calculate button.