Forster Critical Distance Formula

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The Forster Critical Distance is the distance at which the energy transfer efficiency is 50%. Check FAQs
R0=0.0211((η)-4(Φd)(κ2)(J))16
R0 - Forster Critical Distance?η - Refractive Index of Medium?Φd - Fluorescence Quantum Yield without FRET?κ2 - Orientation Factor?J - Spectral Overlap Integral?

Forster Critical Distance Example

With values
With units
Only example

Here is how the Forster Critical Distance equation looks like with Values.

Here is how the Forster Critical Distance equation looks like with Units.

Here is how the Forster Critical Distance equation looks like.

3.8E+7Edit=0.0211((1.3Edit)-4(0.13Edit)(1.5Edit)(0.0005Edit))16
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Forster Critical Distance Solution

Follow our step by step solution on how to calculate Forster Critical Distance?

FIRST Step Consider the formula
R0=0.0211((η)-4(Φd)(κ2)(J))16
Next Step Substitute values of Variables
R0=0.0211((1.3)-4(0.13)(1.5)(0.0005))16
Next Step Prepare to Evaluate
R0=0.0211((1.3)-4(0.13)(1.5)(0.0005))16
Next Step Evaluate
R0=0.00380032719704193m
Next Step Convert to Output's Unit
R0=38003271.9704193A
LAST Step Rounding Answer
R0=3.8E+7A

Forster Critical Distance Formula Elements

Variables
Forster Critical Distance
The Forster Critical Distance is the distance at which the energy transfer efficiency is 50%.
Symbol: R0
Measurement: LengthUnit: A
Note: Value should be greater than 0.
Refractive Index of Medium
Refractive Index of Medium is the refractive index of the donor acceptor medium which is normally between 1.2 to 1.4.
Symbol: η
Measurement: NAUnit: Unitless
Note: Value should be greater than 0.
Fluorescence Quantum Yield without FRET
Fluorescence Quantum Yield without FRET is the fluorescence quantum yield of the donor in the absence of the acceptor.
Symbol: Φd
Measurement: NAUnit: Unitless
Note: Value should be greater than 0.
Orientation Factor
Orientation Factor is the spatial orientation factor describing the relative orientation in the space of the transition dipoles of the donor and acceptor.
Symbol: κ2
Measurement: NAUnit: Unitless
Note: Value should be greater than 0.
Spectral Overlap Integral
Spectral Overlap Integral is the overlap integral of the fluorescence emission spectrum of the donor and the absorption spectrum of the acceptor.
Symbol: J
Measurement: NAUnit: Unitless
Note: Value should be greater than 0.

Other formulas in Förster resonance energy transfer category

​Go Efficiency of Energy Transfer using Distances
E=11+(rR0)6
​Go Efficiency of Energy Transfer using Rate of Energy Transfer
E=KTKT+Knr+Kr
​Go Efficiency of Energy Transfer using Rate of Energy Transfer and Donor Lifetime
E=KT1ζDA
​Go Donor Lifetime using Rates of Transitions
ζD=1Kr+Knr

How to Evaluate Forster Critical Distance?

Forster Critical Distance evaluator uses Forster Critical Distance = 0.0211*((Refractive Index of Medium)^(-4)*(Fluorescence Quantum Yield without FRET)*(Orientation Factor)*(Spectral Overlap Integral))^(1/6) to evaluate the Forster Critical Distance, The Forster Critical Distance formula is defined as the Förster distance of this pair of donor and acceptor, i.e. the distance at which the energy transfer efficiency is 50%. Forster Critical Distance is denoted by R0 symbol.

How to evaluate Forster Critical Distance using this online evaluator? To use this online evaluator for Forster Critical Distance, enter Refractive Index of Medium (η), Fluorescence Quantum Yield without FRET d), Orientation Factor 2) & Spectral Overlap Integral (J) and hit the calculate button.

FAQs on Forster Critical Distance

What is the formula to find Forster Critical Distance?
The formula of Forster Critical Distance is expressed as Forster Critical Distance = 0.0211*((Refractive Index of Medium)^(-4)*(Fluorescence Quantum Yield without FRET)*(Orientation Factor)*(Spectral Overlap Integral))^(1/6). Here is an example- 3.8E+17 = 0.0211*((1.3)^(-4)*(0.13)*(1.5)*(0.0005))^(1/6).
How to calculate Forster Critical Distance?
With Refractive Index of Medium (η), Fluorescence Quantum Yield without FRET d), Orientation Factor 2) & Spectral Overlap Integral (J) we can find Forster Critical Distance using the formula - Forster Critical Distance = 0.0211*((Refractive Index of Medium)^(-4)*(Fluorescence Quantum Yield without FRET)*(Orientation Factor)*(Spectral Overlap Integral))^(1/6).
Can the Forster Critical Distance be negative?
No, the Forster Critical Distance, measured in Length cannot be negative.
Which unit is used to measure Forster Critical Distance?
Forster Critical Distance is usually measured using the Angstrom[A] for Length. Meter[A], Millimeter[A], Kilometer[A] are the few other units in which Forster Critical Distance can be measured.
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