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The Enzyme Substrate Modifying Factor is defined by the inhibitor concentration and the dissociation constant of the enzyme-substrate complex. Check FAQs
α'=(VmaxS)-(V0KM)V0S
α' - Enzyme Substrate Modifying Factor?Vmax - Maximum Rate?S - Substrate Concentration?V0 - Initial Reaction Rate?KM - Michaelis Constant?

Enzyme Substrate Modifying Factor in Presence of Uncompetitive Inhibitor Example

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Here is how the Enzyme Substrate Modifying Factor in Presence of Uncompetitive Inhibitor equation looks like with Values.

Here is how the Enzyme Substrate Modifying Factor in Presence of Uncompetitive Inhibitor equation looks like with Units.

Here is how the Enzyme Substrate Modifying Factor in Presence of Uncompetitive Inhibitor equation looks like.

86.8889Edit=(40Edit1.5Edit)-(0.45Edit3Edit)0.45Edit1.5Edit
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Enzyme Substrate Modifying Factor in Presence of Uncompetitive Inhibitor Solution

Follow our step by step solution on how to calculate Enzyme Substrate Modifying Factor in Presence of Uncompetitive Inhibitor?

FIRST Step Consider the formula
α'=(VmaxS)-(V0KM)V0S
Next Step Substitute values of Variables
α'=(40mol/L*s1.5mol/L)-(0.45mol/L*s3mol/L)0.45mol/L*s1.5mol/L
Next Step Convert Units
α'=(40000mol/m³*s1500mol/m³)-(450mol/m³*s3000mol/m³)450mol/m³*s1500mol/m³
Next Step Prepare to Evaluate
α'=(400001500)-(4503000)4501500
Next Step Evaluate
α'=86.8888888888889
LAST Step Rounding Answer
α'=86.8889

Enzyme Substrate Modifying Factor in Presence of Uncompetitive Inhibitor Formula Elements

Variables
Enzyme Substrate Modifying Factor
The Enzyme Substrate Modifying Factor is defined by the inhibitor concentration and the dissociation constant of the enzyme-substrate complex.
Symbol: α'
Measurement: NAUnit: Unitless
Note: Value can be positive or negative.
Maximum Rate
The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration.
Symbol: Vmax
Measurement: Reaction RateUnit: mol/L*s
Note: Value can be positive or negative.
Substrate Concentration
The Substrate Concentration is the number of moles of substrate per liter solution.
Symbol: S
Measurement: Molar ConcentrationUnit: mol/L
Note: Value can be positive or negative.
Initial Reaction Rate
The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place.
Symbol: V0
Measurement: Reaction RateUnit: mol/L*s
Note: Value can be positive or negative.
Michaelis Constant
The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.
Symbol: KM
Measurement: Molar ConcentrationUnit: mol/L
Note: Value can be positive or negative.

Other Formulas to find Enzyme Substrate Modifying Factor

​Go Enzyme Substrate Modifying Factor given Enzyme Substrate Dissociation Constant
α'=(1+(IKi'))

Other formulas in Uncompetitive Inhibitor category

​Go Substrate Concentration in presence of Uncompetitive Inhibitor
S=KMV0Vmax-(V0α')
​Go Initial Reaction Rate in presence of Uncompetitive Inhibitor
V0=VmaxSKM+(α'S)
​Go Maximum Reaction Rate in Presence of Uncompetitive Inhibitor
Vmax=V0(KM+(α'S))S
​Go Michaelis Constant in Presence of Uncompetitive Inhibitor
KM=S(Vmax-(V0α'))V0

How to Evaluate Enzyme Substrate Modifying Factor in Presence of Uncompetitive Inhibitor?

Enzyme Substrate Modifying Factor in Presence of Uncompetitive Inhibitor evaluator uses Enzyme Substrate Modifying Factor = ((Maximum Rate*Substrate Concentration)-(Initial Reaction Rate*Michaelis Constant))/(Initial Reaction Rate*Substrate Concentration) to evaluate the Enzyme Substrate Modifying Factor, The Enzyme substrate modifying factor in presence of uncompetitive inhibitor formula is defined by the altered Michaelis-Menten equation. Enzyme Substrate Modifying Factor is denoted by α' symbol.

How to evaluate Enzyme Substrate Modifying Factor in Presence of Uncompetitive Inhibitor using this online evaluator? To use this online evaluator for Enzyme Substrate Modifying Factor in Presence of Uncompetitive Inhibitor, enter Maximum Rate (Vmax), Substrate Concentration (S), Initial Reaction Rate (V0) & Michaelis Constant (KM) and hit the calculate button.

FAQs on Enzyme Substrate Modifying Factor in Presence of Uncompetitive Inhibitor

What is the formula to find Enzyme Substrate Modifying Factor in Presence of Uncompetitive Inhibitor?
The formula of Enzyme Substrate Modifying Factor in Presence of Uncompetitive Inhibitor is expressed as Enzyme Substrate Modifying Factor = ((Maximum Rate*Substrate Concentration)-(Initial Reaction Rate*Michaelis Constant))/(Initial Reaction Rate*Substrate Concentration). Here is an example- 86.88889 = ((40000*1500)-(450*3000))/(450*1500).
How to calculate Enzyme Substrate Modifying Factor in Presence of Uncompetitive Inhibitor?
With Maximum Rate (Vmax), Substrate Concentration (S), Initial Reaction Rate (V0) & Michaelis Constant (KM) we can find Enzyme Substrate Modifying Factor in Presence of Uncompetitive Inhibitor using the formula - Enzyme Substrate Modifying Factor = ((Maximum Rate*Substrate Concentration)-(Initial Reaction Rate*Michaelis Constant))/(Initial Reaction Rate*Substrate Concentration).
What are the other ways to Calculate Enzyme Substrate Modifying Factor?
Here are the different ways to Calculate Enzyme Substrate Modifying Factor-
  • Enzyme Substrate Modifying Factor=(1+(Inhibitor Concentration/Enzyme Substrate Dissociation Constant))OpenImg
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