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Enzyme Substrate Dissociation constant in Presence of Uncompetitive Inhibitor Formula

GoEnzyme Substrate Dissociation constant given Enzyme Substrate Modifying factor Formula

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The Enzyme Substrate Dissociation Constant is difficult to measure directly since the enzyme-substrate complex is short-lived and undergoes a chemical reaction to form the product. Check FAQs

K i' = \frac{ES \cdot I}{ESI}

K_i' - Enzyme Substrate Dissociation Constant?ES - Enzyme Substrate Complex Concentration?I - Inhibitor Concentration?ESI - Enzyme Substrate Inhibitor Complex Concentration?

Enzyme Substrate Dissociation constant in Presence of Uncompetitive Inhibitor Example

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Here is how the Enzyme Substrate Dissociation constant in Presence of Uncompetitive Inhibitor equation looks like with Values.

Here is how the Enzyme Substrate Dissociation constant in Presence of Uncompetitive Inhibitor equation looks like with Units.

Here is how the Enzyme Substrate Dissociation constant in Presence of Uncompetitive Inhibitor equation looks like.

36 = \frac{10 \cdot 9}{2.5}

36mol/L = \frac{10mol/L \cdot 9mol/L}{2.5mol/L}

36 = \frac{10 \cdot 9}{2.5}

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Enzyme Substrate Dissociation constant in Presence of Uncompetitive Inhibitor Solution

Follow our step by step solution on how to calculate Enzyme Substrate Dissociation constant in Presence of Uncompetitive Inhibitor?

FIRST Step Consider the formula

K i' = \frac{ES \cdot I}{ESI}

Next Step Substitute values of Variables

∴

K i' = \frac{10mol/L \cdot 9mol/L}{2.5mol/L}

Next Step Convert Units

∴

K i' = \frac{10000mol/m³ \cdot 9000mol/m³}{2500mol/m³}

Next Step Prepare to Evaluate

∴

K i' = \frac{10000 \cdot 9000}{2500}

Next Step Evaluate

∴

K i' = 36000mol/m³

LAST Step Convert to Output's Unit

∴

K i' = 36mol/L

Enzyme Substrate Dissociation constant in Presence of Uncompetitive Inhibitor Formula Elements

Variables

Enzyme Substrate Dissociation Constant

The Enzyme Substrate Dissociation Constant is difficult to measure directly since the enzyme-substrate complex is short-lived and undergoes a chemical reaction to form the product.

Symbol: K_i'

Measurement: Molar ConcentrationUnit: mol/L

Note: Value can be positive or negative.

Formulas to find Enzyme Substrate Dissociation Constant

Enzyme Substrate Complex Concentration

The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate.

Symbol: ES

Measurement: Molar ConcentrationUnit: mol/L

Note: Value can be positive or negative.

Formulas to find Enzyme Substrate Complex Concentration

Inhibitor Concentration

The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system.

Symbol: I

Measurement: Molar ConcentrationUnit: mol/L

Note: Value can be positive or negative.

Formulas to find Inhibitor Concentration

Enzyme Substrate Inhibitor Complex Concentration

The Enzyme Substrate Inhibitor Complex Concentration is the molar concentration of the complex.

Symbol: ESI

Measurement: Molar ConcentrationUnit: mol/L

Note: Value can be positive or negative.

Formulas to find Enzyme Substrate Inhibitor Complex Concentration

Other Formulas to find Enzyme Substrate Dissociation Constant

Go Enzyme Substrate Dissociation constant given Enzyme Substrate Modifying factor

K i' = \frac{I}{α' - 1}

Other formulas in Uncompetitive Inhibitor category

Go Substrate Concentration in presence of Uncompetitive Inhibitor

S = \frac{K M \cdot V 0}{V max - (V 0 \cdot α')}

Go Initial Reaction Rate in presence of Uncompetitive Inhibitor

V 0 = \frac{V max \cdot S}{K M + (α' \cdot S)}

Go Maximum Reaction Rate in Presence of Uncompetitive Inhibitor

V max = \frac{V 0 \cdot (K M + (α' \cdot S))}{S}

Go Michaelis Constant in Presence of Uncompetitive Inhibitor

K M = \frac{S \cdot (V max - (V 0 \cdot α'))}{V 0}

How to Evaluate Enzyme Substrate Dissociation constant in Presence of Uncompetitive Inhibitor?

Enzyme Substrate Dissociation constant in Presence of Uncompetitive Inhibitor evaluator uses Enzyme Substrate Dissociation Constant = (Enzyme Substrate Complex Concentration*Inhibitor Concentration)/Enzyme Substrate Inhibitor Complex Concentration to evaluate the Enzyme Substrate Dissociation Constant, The Enzyme substrate dissociation constant in presence of uncompetitive inhibitor formula is defined as related to the concentration of different enzyme species. Enzyme Substrate Dissociation Constant is denoted by K_i' symbol.

How to evaluate Enzyme Substrate Dissociation constant in Presence of Uncompetitive Inhibitor using this online evaluator? To use this online evaluator for Enzyme Substrate Dissociation constant in Presence of Uncompetitive Inhibitor, enter Enzyme Substrate Complex Concentration (ES), Inhibitor Concentration (I) & Enzyme Substrate Inhibitor Complex Concentration (ESI) and hit the calculate button.

FAQs on Enzyme Substrate Dissociation constant in Presence of Uncompetitive Inhibitor

What is the formula to find Enzyme Substrate Dissociation constant in Presence of Uncompetitive Inhibitor?

The formula of Enzyme Substrate Dissociation constant in Presence of Uncompetitive Inhibitor is expressed as Enzyme Substrate Dissociation Constant = (Enzyme Substrate Complex Concentration*Inhibitor Concentration)/Enzyme Substrate Inhibitor Complex Concentration. Here is an example- 0.036 = (10000*9000)/2500.

How to calculate Enzyme Substrate Dissociation constant in Presence of Uncompetitive Inhibitor?

With Enzyme Substrate Complex Concentration (ES), Inhibitor Concentration (I) & Enzyme Substrate Inhibitor Complex Concentration (ESI) we can find Enzyme Substrate Dissociation constant in Presence of Uncompetitive Inhibitor using the formula - Enzyme Substrate Dissociation Constant = (Enzyme Substrate Complex Concentration*Inhibitor Concentration)/Enzyme Substrate Inhibitor Complex Concentration.

What are the other ways to Calculate Enzyme Substrate Dissociation Constant?

Here are the different ways to Calculate Enzyme Substrate Dissociation Constant-

Enzyme Substrate Dissociation Constant=Inhibitor Concentration/(Enzyme Substrate Modifying Factor-1)

Can the Enzyme Substrate Dissociation constant in Presence of Uncompetitive Inhibitor be negative?

Yes, the Enzyme Substrate Dissociation constant in Presence of Uncompetitive Inhibitor, measured in Molar Concentration can be negative.

Which unit is used to measure Enzyme Substrate Dissociation constant in Presence of Uncompetitive Inhibitor?

Enzyme Substrate Dissociation constant in Presence of Uncompetitive Inhibitor is usually measured using the Mole per Liter[mol/L] for Molar Concentration. Mole per Cubic Meter[mol/L], Mole per Cubic Millimeter[mol/L], Kilomole per Cubic Meter[mol/L] are the few other units in which Enzyme Substrate Dissociation constant in Presence of Uncompetitive Inhibitor can be measured.

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Enzyme Substrate Dissociation constant in Presence of Uncompetitive Inhibitor Formula

​GoEnzyme Substrate Dissociation constant given Enzyme Substrate Modifying factor Formula

Enzyme Substrate Dissociation constant in Presence of Uncompetitive Inhibitor Example

Enzyme Substrate Dissociation constant in Presence of Uncompetitive Inhibitor Solution

Enzyme Substrate Dissociation constant in Presence of Uncompetitive Inhibitor Formula Elements

Other Formulas to find Enzyme Substrate Dissociation Constant

Other formulas in Uncompetitive Inhibitor category

How to Evaluate Enzyme Substrate Dissociation constant in Presence of Uncompetitive Inhibitor?

FAQs on Enzyme Substrate Dissociation constant in Presence of Uncompetitive Inhibitor

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GoEnzyme Substrate Dissociation constant given Enzyme Substrate Modifying factor Formula