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Enzyme Substrate Complex Concentration for Competitive Inhibition of Enzyme Catalysis Formula

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The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate. Check FAQs

ES = \frac{S \cdot [E 0]}{K M \cdot (1 + (\frac{I}{K i})) + S}

ES - Enzyme Substrate Complex Concentration?S - Substrate Concentration?[E₀] - Initial Enzyme Concentration?K_M - Michaelis Constant?I - Inhibitor Concentration?K_i - Enzyme Inhibitor Dissociation Constant?

Enzyme Substrate Complex Concentration for Competitive Inhibition of Enzyme Catalysis Example

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Here is how the Enzyme Substrate Complex Concentration for Competitive Inhibition of Enzyme Catalysis equation looks like with Values.

Here is how the Enzyme Substrate Complex Concentration for Competitive Inhibition of Enzyme Catalysis equation looks like with Units.

Here is how the Enzyme Substrate Complex Concentration for Competitive Inhibition of Enzyme Catalysis equation looks like.

25.3333 = \frac{1.5 \cdot 100}{3 \cdot (1 + (\frac{9}{19})) + 1.5}

25.3333mol/L = \frac{1.5mol/L \cdot 100mol/L}{3mol/L \cdot (1 + (\frac{9mol/L}{19mol/L})) + 1.5mol/L}

25.3333 = \frac{1.5 \cdot 100}{3 \cdot (1 + (\frac{9}{19})) + 1.5}

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Enzyme Substrate Complex Concentration for Competitive Inhibition of Enzyme Catalysis Solution

Follow our step by step solution on how to calculate Enzyme Substrate Complex Concentration for Competitive Inhibition of Enzyme Catalysis?

FIRST Step Consider the formula

ES = \frac{S \cdot [E 0]}{K M \cdot (1 + (\frac{I}{K i})) + S}

Next Step Substitute values of Variables

∴

ES = \frac{1.5mol/L \cdot 100mol/L}{3mol/L \cdot (1 + (\frac{9mol/L}{19mol/L})) + 1.5mol/L}

Next Step Convert Units

∴

ES = \frac{1500mol/m³ \cdot 100000mol/m³}{3000mol/m³ \cdot (1 + (\frac{9000mol/m³}{19000mol/m³})) + 1500mol/m³}

Next Step Prepare to Evaluate

∴

ES = \frac{1500 \cdot 100000}{3000 \cdot (1 + (\frac{9000}{19000})) + 1500}

Next Step Evaluate

∴

ES = 25333.3333333333mol/m³

Next Step Convert to Output's Unit

∴

ES = 25.3333333333333mol/L

LAST Step Rounding Answer

∴

ES = 25.3333mol/L

Enzyme Substrate Complex Concentration for Competitive Inhibition of Enzyme Catalysis Formula Elements

Variables

Enzyme Substrate Complex Concentration

The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate.

Symbol: ES

Measurement: Molar ConcentrationUnit: mol/L

Note: Value can be positive or negative.

Formulas to find Enzyme Substrate Complex Concentration

Substrate Concentration

The Substrate Concentration is the number of moles of substrate per liter solution.

Symbol: S

Measurement: Molar ConcentrationUnit: mol/L

Note: Value can be positive or negative.

Formulas to find Substrate Concentration

Initial Enzyme Concentration

The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction.

Symbol: [E₀]

Measurement: Molar ConcentrationUnit: mol/L

Note: Value should be greater than 0.

Formulas to find Initial Enzyme Concentration

Michaelis Constant

The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.

Symbol: K_M

Measurement: Molar ConcentrationUnit: mol/L

Note: Value can be positive or negative.

Formulas to find Michaelis Constant

Inhibitor Concentration

The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system.

Symbol: I

Measurement: Molar ConcentrationUnit: mol/L

Note: Value can be positive or negative.

Formulas to find Inhibitor Concentration

Enzyme Inhibitor Dissociation Constant

The Enzyme Inhibitor Dissociation Constant is measured by the method in which the inhibitor is titrated into a solution of enzyme and the heat released or absorbed is measured.

Symbol: K_i

Measurement: Molar ConcentrationUnit: mol/L

Note: Value can be positive or negative.

Formulas to find Enzyme Inhibitor Dissociation Constant

Other formulas in Competitive Inhibitor category

Go Modifying Factor of Enzyme Substrate Complex

α' = 1 + (\frac{I}{K i'})

Go Initial Rate of System given Rate Constant and Enzyme Substrate Complex Concentration

V RC = k 2 \cdot ES

Go Initial Reaction Rate given Dissociation Rate Constant

V DRC = \frac{V max \cdot S}{K D + S}

Go Maximum Rate given Dissociation Rate Constant

V max_DRC = \frac{V 0 \cdot (K D + S)}{S}

How to Evaluate Enzyme Substrate Complex Concentration for Competitive Inhibition of Enzyme Catalysis?

Enzyme Substrate Complex Concentration for Competitive Inhibition of Enzyme Catalysis evaluator uses Enzyme Substrate Complex Concentration = (Substrate Concentration*Initial Enzyme Concentration)/(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))+Substrate Concentration) to evaluate the Enzyme Substrate Complex Concentration, The Enzyme substrate complex concentration for competitive inhibition of enzyme catalysis formula is defined as a plot of the reaction velocity (V0) associated with the concentration [S] of the substrate which can then be used to determine values such as Vmax, initial velocity, and Km. Enzyme Substrate Complex Concentration is denoted by ES symbol.

How to evaluate Enzyme Substrate Complex Concentration for Competitive Inhibition of Enzyme Catalysis using this online evaluator? To use this online evaluator for Enzyme Substrate Complex Concentration for Competitive Inhibition of Enzyme Catalysis, enter Substrate Concentration (S), Initial Enzyme Concentration ([E₀]), Michaelis Constant (K_M), Inhibitor Concentration (I) & Enzyme Inhibitor Dissociation Constant (K_i) and hit the calculate button.

FAQs on Enzyme Substrate Complex Concentration for Competitive Inhibition of Enzyme Catalysis

What is the formula to find Enzyme Substrate Complex Concentration for Competitive Inhibition of Enzyme Catalysis?

The formula of Enzyme Substrate Complex Concentration for Competitive Inhibition of Enzyme Catalysis is expressed as Enzyme Substrate Complex Concentration = (Substrate Concentration*Initial Enzyme Concentration)/(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))+Substrate Concentration). Here is an example- 0.025333 = (1500*100000)/(3000*(1+(9000/19000))+1500).

How to calculate Enzyme Substrate Complex Concentration for Competitive Inhibition of Enzyme Catalysis?

With Substrate Concentration (S), Initial Enzyme Concentration ([E₀]), Michaelis Constant (K_M), Inhibitor Concentration (I) & Enzyme Inhibitor Dissociation Constant (K_i) we can find Enzyme Substrate Complex Concentration for Competitive Inhibition of Enzyme Catalysis using the formula - Enzyme Substrate Complex Concentration = (Substrate Concentration*Initial Enzyme Concentration)/(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))+Substrate Concentration).

Can the Enzyme Substrate Complex Concentration for Competitive Inhibition of Enzyme Catalysis be negative?

Yes, the Enzyme Substrate Complex Concentration for Competitive Inhibition of Enzyme Catalysis, measured in Molar Concentration can be negative.

Which unit is used to measure Enzyme Substrate Complex Concentration for Competitive Inhibition of Enzyme Catalysis?

Enzyme Substrate Complex Concentration for Competitive Inhibition of Enzyme Catalysis is usually measured using the Mole per Liter[mol/L] for Molar Concentration. Mole per Cubic Meter[mol/L], Mole per Cubic Millimeter[mol/L], Kilomole per Cubic Meter[mol/L] are the few other units in which Enzyme Substrate Complex Concentration for Competitive Inhibition of Enzyme Catalysis can be measured.

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Enzyme Substrate Complex Concentration for Competitive Inhibition of Enzyme Catalysis Formula

Enzyme Substrate Complex Concentration for Competitive Inhibition of Enzyme Catalysis Example

Enzyme Substrate Complex Concentration for Competitive Inhibition of Enzyme Catalysis Solution

Enzyme Substrate Complex Concentration for Competitive Inhibition of Enzyme Catalysis Formula Elements

Other formulas in Competitive Inhibitor category

How to Evaluate Enzyme Substrate Complex Concentration for Competitive Inhibition of Enzyme Catalysis?

FAQs on Enzyme Substrate Complex Concentration for Competitive Inhibition of Enzyme Catalysis

Variable Name