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The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate. Check FAQs
ES=kfESkr+kcat
ES - Enzyme Substrate Complex Concentration?kf - Forward Rate Constant?E - Catalyst Concentration?S - Substrate Concentration?kr - Reverse Rate Constant?kcat - Catalytic Rate Constant?

Enzyme Substate complex Concentration given Forward, Reverse, and Catalytic Rate Constant Example

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With units
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Here is how the Enzyme Substate complex Concentration given Forward, Reverse, and Catalytic Rate Constant equation looks like with Values.

Here is how the Enzyme Substate complex Concentration given Forward, Reverse, and Catalytic Rate Constant equation looks like with Units.

Here is how the Enzyme Substate complex Concentration given Forward, Reverse, and Catalytic Rate Constant equation looks like.

12.9371Edit=6.9Edit25Edit1.5Edit20Edit+0.65Edit
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Enzyme Substate complex Concentration given Forward, Reverse, and Catalytic Rate Constant Solution

Follow our step by step solution on how to calculate Enzyme Substate complex Concentration given Forward, Reverse, and Catalytic Rate Constant?

FIRST Step Consider the formula
ES=kfESkr+kcat
Next Step Substitute values of Variables
ES=6.9s⁻¹25mol/L1.5mol/L20mol/L*s+0.65s⁻¹
Next Step Convert Units
ES=6.9s⁻¹25000mol/m³1500mol/m³20000mol/m³*s+0.65s⁻¹
Next Step Prepare to Evaluate
ES=6.925000150020000+0.65
Next Step Evaluate
ES=12937.0795449148mol/m³
Next Step Convert to Output's Unit
ES=12.9370795449148mol/L
LAST Step Rounding Answer
ES=12.9371mol/L

Enzyme Substate complex Concentration given Forward, Reverse, and Catalytic Rate Constant Formula Elements

Variables
Enzyme Substrate Complex Concentration
The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate.
Symbol: ES
Measurement: Molar ConcentrationUnit: mol/L
Note: Value can be positive or negative.
Forward Rate Constant
The Forward Rate Constant is defined as the rate constant for the forward occurring reaction.
Symbol: kf
Measurement: First Order Reaction Rate ConstantUnit: s⁻¹
Note: Value can be positive or negative.
Catalyst Concentration
The Catalyst Concentration is the number of moles of catalyst present in the per liter of solution.
Symbol: E
Measurement: Molar ConcentrationUnit: mol/L
Note: Value can be positive or negative.
Substrate Concentration
The Substrate Concentration is the number of moles of substrate per liter solution.
Symbol: S
Measurement: Molar ConcentrationUnit: mol/L
Note: Value can be positive or negative.
Reverse Rate Constant
The Reverse Rate Constant is defined as the rate constant for the backward reaction.
Symbol: kr
Measurement: Reaction RateUnit: mol/L*s
Note: Value can be positive or negative.
Catalytic Rate Constant
The Catalytic Rate Constant is defined as the rate constant for conversion of the enzyme-substrate complex to enzyme and product.
Symbol: kcat
Measurement: First Order Reaction Rate ConstantUnit: s⁻¹
Note: Value can be positive or negative.

Other Formulas to find Enzyme Substrate Complex Concentration

​Go Enzyme Substrate complex Concentration in Presence of Uncompetitive Inhibitor
ES=Ki'ESII

Other formulas in Uncompetitive Inhibitor category

​Go Substrate Concentration in presence of Uncompetitive Inhibitor
S=KMV0Vmax-(V0α')
​Go Initial Reaction Rate in presence of Uncompetitive Inhibitor
V0=VmaxSKM+(α'S)
​Go Maximum Reaction Rate in Presence of Uncompetitive Inhibitor
Vmax=V0(KM+(α'S))S
​Go Michaelis Constant in Presence of Uncompetitive Inhibitor
KM=S(Vmax-(V0α'))V0

How to Evaluate Enzyme Substate complex Concentration given Forward, Reverse, and Catalytic Rate Constant?

Enzyme Substate complex Concentration given Forward, Reverse, and Catalytic Rate Constant evaluator uses Enzyme Substrate Complex Concentration = (Forward Rate Constant*Catalyst Concentration*Substrate Concentration)/(Reverse Rate Constant+Catalytic Rate Constant) to evaluate the Enzyme Substrate Complex Concentration, The Enzyme substate complex concentration given forward, reverse, and catalytic rate constants formula is defined as the relation with enzymatic catalyst and with substrate concentration in an enzymatic reaction mechanism. Enzyme Substrate Complex Concentration is denoted by ES symbol.

How to evaluate Enzyme Substate complex Concentration given Forward, Reverse, and Catalytic Rate Constant using this online evaluator? To use this online evaluator for Enzyme Substate complex Concentration given Forward, Reverse, and Catalytic Rate Constant, enter Forward Rate Constant (kf), Catalyst Concentration (E), Substrate Concentration (S), Reverse Rate Constant (kr) & Catalytic Rate Constant (kcat) and hit the calculate button.

FAQs on Enzyme Substate complex Concentration given Forward, Reverse, and Catalytic Rate Constant

What is the formula to find Enzyme Substate complex Concentration given Forward, Reverse, and Catalytic Rate Constant?
The formula of Enzyme Substate complex Concentration given Forward, Reverse, and Catalytic Rate Constant is expressed as Enzyme Substrate Complex Concentration = (Forward Rate Constant*Catalyst Concentration*Substrate Concentration)/(Reverse Rate Constant+Catalytic Rate Constant). Here is an example- 0.012937 = (6.9*25000*1500)/(20000+0.65).
How to calculate Enzyme Substate complex Concentration given Forward, Reverse, and Catalytic Rate Constant?
With Forward Rate Constant (kf), Catalyst Concentration (E), Substrate Concentration (S), Reverse Rate Constant (kr) & Catalytic Rate Constant (kcat) we can find Enzyme Substate complex Concentration given Forward, Reverse, and Catalytic Rate Constant using the formula - Enzyme Substrate Complex Concentration = (Forward Rate Constant*Catalyst Concentration*Substrate Concentration)/(Reverse Rate Constant+Catalytic Rate Constant).
What are the other ways to Calculate Enzyme Substrate Complex Concentration?
Here are the different ways to Calculate Enzyme Substrate Complex Concentration-
  • Enzyme Substrate Complex Concentration=(Enzyme Substrate Dissociation Constant*Enzyme Substrate Inhibitor Complex Concentration)/Inhibitor ConcentrationOpenImg
Can the Enzyme Substate complex Concentration given Forward, Reverse, and Catalytic Rate Constant be negative?
Yes, the Enzyme Substate complex Concentration given Forward, Reverse, and Catalytic Rate Constant, measured in Molar Concentration can be negative.
Which unit is used to measure Enzyme Substate complex Concentration given Forward, Reverse, and Catalytic Rate Constant?
Enzyme Substate complex Concentration given Forward, Reverse, and Catalytic Rate Constant is usually measured using the Mole per Liter[mol/L] for Molar Concentration. Mole per Cubic Meter[mol/L], Mole per Cubic Millimeter[mol/L], Kilomole per Cubic Meter[mol/L] are the few other units in which Enzyme Substate complex Concentration given Forward, Reverse, and Catalytic Rate Constant can be measured.
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