Enzyme Concentration from Michaelis Menten Kinetics equation Formula

Fx Copy
LaTeX Copy
Initial Concentration of Enzyme is defined as the concentration of enzyme at the start of the reaction. Check FAQs
[Ei]=V0(KM+S)kcatS
[Ei] - Initial Concentration of Enzyme?V0 - Initial Reaction Rate?KM - Michaelis Constant?S - Substrate Concentration?kcat - Catalytic Rate Constant?

Enzyme Concentration from Michaelis Menten Kinetics equation Example

With values
With units
Only example

Here is how the Enzyme Concentration from Michaelis Menten Kinetics equation equation looks like with Values.

Here is how the Enzyme Concentration from Michaelis Menten Kinetics equation equation looks like with Units.

Here is how the Enzyme Concentration from Michaelis Menten Kinetics equation equation looks like.

2.0769Edit=0.45Edit(3Edit+1.5Edit)0.65Edit1.5Edit
You are here -
HomeIcon Home » Category Chemistry » Category Chemical Kinetics » Category Enzyme Kinetics » fx Enzyme Concentration from Michaelis Menten Kinetics equation

Enzyme Concentration from Michaelis Menten Kinetics equation Solution

Follow our step by step solution on how to calculate Enzyme Concentration from Michaelis Menten Kinetics equation?

FIRST Step Consider the formula
[Ei]=V0(KM+S)kcatS
Next Step Substitute values of Variables
[Ei]=0.45mol/L*s(3mol/L+1.5mol/L)0.65s⁻¹1.5mol/L
Next Step Convert Units
[Ei]=450mol/m³*s(3000mol/m³+1500mol/m³)0.65s⁻¹1500mol/m³
Next Step Prepare to Evaluate
[Ei]=450(3000+1500)0.651500
Next Step Evaluate
[Ei]=2076.92307692308mol/m³
Next Step Convert to Output's Unit
[Ei]=2.07692307692308mol/L
LAST Step Rounding Answer
[Ei]=2.0769mol/L

Enzyme Concentration from Michaelis Menten Kinetics equation Formula Elements

Variables
Initial Concentration of Enzyme
Initial Concentration of Enzyme is defined as the concentration of enzyme at the start of the reaction.
Symbol: [Ei]
Measurement: Molar ConcentrationUnit: mol/L
Note: Value can be positive or negative.
Initial Reaction Rate
The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place.
Symbol: V0
Measurement: Reaction RateUnit: mol/L*s
Note: Value can be positive or negative.
Michaelis Constant
The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.
Symbol: KM
Measurement: Molar ConcentrationUnit: mol/L
Note: Value can be positive or negative.
Substrate Concentration
The Substrate Concentration is the number of moles of substrate per liter solution.
Symbol: S
Measurement: Molar ConcentrationUnit: mol/L
Note: Value can be positive or negative.
Catalytic Rate Constant
The Catalytic Rate Constant is defined as the rate constant for conversion of the enzyme-substrate complex to enzyme and product.
Symbol: kcat
Measurement: First Order Reaction Rate ConstantUnit: s⁻¹
Note: Value can be positive or negative.

Other formulas in Michaelis Menten Kinetics Equation category

​Go Modifying Factor of Enzyme Substrate Complex
α'=1+(IKi')
​Go Initial Rate of System given Rate Constant and Enzyme Substrate Complex Concentration
VRC=k2ES
​Go Initial Reaction Rate given Dissociation Rate Constant
VDRC=VmaxSKD+S
​Go Maximum Rate given Dissociation Rate Constant
Vmax_DRC=V0(KD+S)S

How to Evaluate Enzyme Concentration from Michaelis Menten Kinetics equation?

Enzyme Concentration from Michaelis Menten Kinetics equation evaluator uses Initial Concentration of Enzyme = (Initial Reaction Rate*(Michaelis Constant+Substrate Concentration))/(Catalytic Rate Constant*Substrate Concentration) to evaluate the Initial Concentration of Enzyme, The Enzyme concentration from Michaelis Menten kinetics equation formula is defined as the relation with initial reaction rate and the concentration of a substrate. Initial Concentration of Enzyme is denoted by [Ei] symbol.

How to evaluate Enzyme Concentration from Michaelis Menten Kinetics equation using this online evaluator? To use this online evaluator for Enzyme Concentration from Michaelis Menten Kinetics equation, enter Initial Reaction Rate (V0), Michaelis Constant (KM), Substrate Concentration (S) & Catalytic Rate Constant (kcat) and hit the calculate button.

FAQs on Enzyme Concentration from Michaelis Menten Kinetics equation

What is the formula to find Enzyme Concentration from Michaelis Menten Kinetics equation?
The formula of Enzyme Concentration from Michaelis Menten Kinetics equation is expressed as Initial Concentration of Enzyme = (Initial Reaction Rate*(Michaelis Constant+Substrate Concentration))/(Catalytic Rate Constant*Substrate Concentration). Here is an example- 0.002077 = (450*(3000+1500))/(0.65*1500).
How to calculate Enzyme Concentration from Michaelis Menten Kinetics equation?
With Initial Reaction Rate (V0), Michaelis Constant (KM), Substrate Concentration (S) & Catalytic Rate Constant (kcat) we can find Enzyme Concentration from Michaelis Menten Kinetics equation using the formula - Initial Concentration of Enzyme = (Initial Reaction Rate*(Michaelis Constant+Substrate Concentration))/(Catalytic Rate Constant*Substrate Concentration).
Can the Enzyme Concentration from Michaelis Menten Kinetics equation be negative?
Yes, the Enzyme Concentration from Michaelis Menten Kinetics equation, measured in Molar Concentration can be negative.
Which unit is used to measure Enzyme Concentration from Michaelis Menten Kinetics equation?
Enzyme Concentration from Michaelis Menten Kinetics equation is usually measured using the Mole per Liter[mol/L] for Molar Concentration. Mole per Cubic Meter[mol/L], Mole per Cubic Millimeter[mol/L], Kilomole per Cubic Meter[mol/L] are the few other units in which Enzyme Concentration from Michaelis Menten Kinetics equation can be measured.
Copied!