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Dissociation Constant given Enzyme Substrate Complex Concentration Formula

GoDissociation Constant given Apparent Initial Enzyme Concentration Formula

GoDissociation Constant in presence of Noncompetitive Inhibitor Formula

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The Enzyme Inhibitor Dissociation Constant is measured by the method in which the inhibitor is titrated into a solution of enzyme and the heat released or absorbed is measured. Check FAQs

K i = \frac{I}{(\frac{(\frac{[E 0] \cdot S}{ES}) - S}{K M}) - 1}

K_i - Enzyme Inhibitor Dissociation Constant?I - Inhibitor Concentration?[E₀] - Initial Enzyme Concentration?S - Substrate Concentration?ES - Enzyme Substrate Complex Concentration?K_M - Michaelis Constant?

Dissociation Constant given Enzyme Substrate Complex Concentration Example

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Here is how the Dissociation Constant given Enzyme Substrate Complex Concentration equation looks like with Values.

Here is how the Dissociation Constant given Enzyme Substrate Complex Concentration equation looks like with Units.

Here is how the Dissociation Constant given Enzyme Substrate Complex Concentration equation looks like.

2.5714 = \frac{9}{(\frac{(\frac{100 \cdot 1.5}{10}) - 1.5}{3}) - 1}

2.5714mol/L = \frac{9mol/L}{(\frac{(\frac{100mol/L \cdot 1.5mol/L}{10mol/L}) - 1.5mol/L}{3mol/L}) - 1}

2.5714 = \frac{9}{(\frac{(\frac{100 \cdot 1.5}{10}) - 1.5}{3}) - 1}

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Dissociation Constant given Enzyme Substrate Complex Concentration Solution

Follow our step by step solution on how to calculate Dissociation Constant given Enzyme Substrate Complex Concentration?

FIRST Step Consider the formula

K i = \frac{I}{(\frac{(\frac{[E 0] \cdot S}{ES}) - S}{K M}) - 1}

Next Step Substitute values of Variables

∴

K i = \frac{9mol/L}{(\frac{(\frac{100mol/L \cdot 1.5mol/L}{10mol/L}) - 1.5mol/L}{3mol/L}) - 1}

Next Step Convert Units

∴

K i = \frac{9000mol/m³}{(\frac{(\frac{100000mol/m³ \cdot 1500mol/m³}{10000mol/m³}) - 1500mol/m³}{3000mol/m³}) - 1}

Next Step Prepare to Evaluate

∴

K i = \frac{9000}{(\frac{(\frac{100000 \cdot 1500}{10000}) - 1500}{3000}) - 1}

Next Step Evaluate

∴

K i = 2571.42857142857mol/m³

Next Step Convert to Output's Unit

∴

K i = 2.57142857142857mol/L

LAST Step Rounding Answer

∴

K i = 2.5714mol/L

Dissociation Constant given Enzyme Substrate Complex Concentration Formula Elements

Variables

Enzyme Inhibitor Dissociation Constant

The Enzyme Inhibitor Dissociation Constant is measured by the method in which the inhibitor is titrated into a solution of enzyme and the heat released or absorbed is measured.

Symbol: K_i

Measurement: Molar ConcentrationUnit: mol/L

Note: Value can be positive or negative.

Formulas to find Enzyme Inhibitor Dissociation Constant

Inhibitor Concentration

The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system.

Symbol: I

Measurement: Molar ConcentrationUnit: mol/L

Note: Value can be positive or negative.

Formulas to find Inhibitor Concentration

Initial Enzyme Concentration

The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction.

Symbol: [E₀]

Measurement: Molar ConcentrationUnit: mol/L

Note: Value should be greater than 0.

Formulas to find Initial Enzyme Concentration

Substrate Concentration

The Substrate Concentration is the number of moles of substrate per liter solution.

Symbol: S

Measurement: Molar ConcentrationUnit: mol/L

Note: Value can be positive or negative.

Formulas to find Substrate Concentration

Enzyme Substrate Complex Concentration

The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate.

Symbol: ES

Measurement: Molar ConcentrationUnit: mol/L

Note: Value can be positive or negative.

Formulas to find Enzyme Substrate Complex Concentration

Michaelis Constant

The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.

Symbol: K_M

Measurement: Molar ConcentrationUnit: mol/L

Note: Value can be positive or negative.

Formulas to find Michaelis Constant

Other Formulas to find Enzyme Inhibitor Dissociation Constant

Go Dissociation Constant given Apparent Initial Enzyme Concentration

K i = (\frac{I}{(\frac{[E 0]}{E0 app}) - 1})

Go Dissociation Constant in presence of Noncompetitive Inhibitor

K i = (\frac{I}{(\frac{V max}{Vmax app}) - 1})

Other formulas in Noncompetitive Inhibitor category

Go Apparent Initial Enzyme Concentration in Presence of Noncompetitive Inhibitor

E0 app = (\frac{[E 0]}{1 + (\frac{I}{K i})})

Go Apparent Maximum Rate in presence of Noncompetitive Inhibitor

Vmax app = (\frac{V max}{1 + (\frac{I}{K i})})

Go Apparent Michaelis Menten constant given Inhibitor's Dissociation Constant

Km app = K M \cdot (1 + (\frac{I}{K i}))

Go Maximum Rate in presence of Noncompetitive Inhibitor

V max = (Vmax app \cdot (1 + (\frac{I}{K i})))

How to Evaluate Dissociation Constant given Enzyme Substrate Complex Concentration?

Dissociation Constant given Enzyme Substrate Complex Concentration evaluator uses Enzyme Inhibitor Dissociation Constant = Inhibitor Concentration/(((((Initial Enzyme Concentration*Substrate Concentration)/Enzyme Substrate Complex Concentration)-Substrate Concentration)/Michaelis Constant)-1) to evaluate the Enzyme Inhibitor Dissociation Constant, The Dissociation constant given enzyme substrate complex concentrationformula is defined as a plot of the reaction velocity (V0) associated with the concentration [S] of the substrate can then be used to determine values such as Vmax, initial velocity, and Km. Enzyme Inhibitor Dissociation Constant is denoted by K_i symbol.

How to evaluate Dissociation Constant given Enzyme Substrate Complex Concentration using this online evaluator? To use this online evaluator for Dissociation Constant given Enzyme Substrate Complex Concentration, enter Inhibitor Concentration (I), Initial Enzyme Concentration ([E₀]), Substrate Concentration (S), Enzyme Substrate Complex Concentration (ES) & Michaelis Constant (K_M) and hit the calculate button.

FAQs on Dissociation Constant given Enzyme Substrate Complex Concentration

What is the formula to find Dissociation Constant given Enzyme Substrate Complex Concentration?

The formula of Dissociation Constant given Enzyme Substrate Complex Concentration is expressed as Enzyme Inhibitor Dissociation Constant = Inhibitor Concentration/(((((Initial Enzyme Concentration*Substrate Concentration)/Enzyme Substrate Complex Concentration)-Substrate Concentration)/Michaelis Constant)-1). Here is an example- 0.002571 = 9000/(((((100000*1500)/10000)-1500)/3000)-1).

How to calculate Dissociation Constant given Enzyme Substrate Complex Concentration?

With Inhibitor Concentration (I), Initial Enzyme Concentration ([E₀]), Substrate Concentration (S), Enzyme Substrate Complex Concentration (ES) & Michaelis Constant (K_M) we can find Dissociation Constant given Enzyme Substrate Complex Concentration using the formula - Enzyme Inhibitor Dissociation Constant = Inhibitor Concentration/(((((Initial Enzyme Concentration*Substrate Concentration)/Enzyme Substrate Complex Concentration)-Substrate Concentration)/Michaelis Constant)-1).

What are the other ways to Calculate Enzyme Inhibitor Dissociation Constant?

Here are the different ways to Calculate Enzyme Inhibitor Dissociation Constant-

Enzyme Inhibitor Dissociation Constant=(Inhibitor Concentration/((Initial Enzyme Concentration/Apparent Initial Enzyme Concentration)-1))
Enzyme Inhibitor Dissociation Constant=(Inhibitor Concentration/((Maximum Rate/Apparent Maximum Rate)-1))

Can the Dissociation Constant given Enzyme Substrate Complex Concentration be negative?

Yes, the Dissociation Constant given Enzyme Substrate Complex Concentration, measured in Molar Concentration can be negative.

Which unit is used to measure Dissociation Constant given Enzyme Substrate Complex Concentration?

Dissociation Constant given Enzyme Substrate Complex Concentration is usually measured using the Mole per Liter[mol/L] for Molar Concentration. Mole per Cubic Meter[mol/L], Mole per Cubic Millimeter[mol/L], Kilomole per Cubic Meter[mol/L] are the few other units in which Dissociation Constant given Enzyme Substrate Complex Concentration can be measured.

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Dissociation Constant given Enzyme Substrate Complex Concentration Formula

​GoDissociation Constant given Apparent Initial Enzyme Concentration Formula

​GoDissociation Constant in presence of Noncompetitive Inhibitor Formula

Dissociation Constant given Enzyme Substrate Complex Concentration Example

Dissociation Constant given Enzyme Substrate Complex Concentration Solution

Dissociation Constant given Enzyme Substrate Complex Concentration Formula Elements

Other Formulas to find Enzyme Inhibitor Dissociation Constant

Other formulas in Noncompetitive Inhibitor category

How to Evaluate Dissociation Constant given Enzyme Substrate Complex Concentration?

FAQs on Dissociation Constant given Enzyme Substrate Complex Concentration

Variable Name

GoDissociation Constant given Apparent Initial Enzyme Concentration Formula

GoDissociation Constant in presence of Noncompetitive Inhibitor Formula