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Column Efficiency in Chromatography Formula

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Column Efficiency, also known as plate count, is a measure of the dispersion of a peak. Check FAQs

N = 16 \cdot ({(\frac{V R}{W b})}^{2})

N - Column Efficiency?V_R - Retention Volume?W_b - Width of Chromatographic Peak?

Column Efficiency in Chromatography Example

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Here is how the Column Efficiency in Chromatography equation looks like with Values.

Here is how the Column Efficiency in Chromatography equation looks like with Units.

Here is how the Column Efficiency in Chromatography equation looks like.

3.2113 = 16 \cdot ({(\frac{11.2}{25})}^{2})

3.2113 = 16 \cdot ({(\frac{11.2L}{25mm})}^{2})

3.2113 = 16 \cdot ({(\frac{11.2}{25})}^{2})

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Column Efficiency in Chromatography Solution

Follow our step by step solution on how to calculate Column Efficiency in Chromatography?

FIRST Step Consider the formula

N = 16 \cdot ({(\frac{V R}{W b})}^{2})

Next Step Substitute values of Variables

∴

N = 16 \cdot ({(\frac{11.2L}{25mm})}^{2})

Next Step Convert Units

∴

N = 16 \cdot ({(\frac{0.0112m³}{0.025m})}^{2})

Next Step Prepare to Evaluate

∴

N = 16 \cdot ({(\frac{0.0112}{0.025})}^{2})

Next Step Evaluate

∴

N = 3.211264

LAST Step Rounding Answer

∴

N = 3.2113

Column Efficiency in Chromatography Formula Elements

Variables

Column Efficiency

Column Efficiency, also known as plate count, is a measure of the dispersion of a peak.

Symbol: N

Measurement: NAUnit: Unitless

Note: Value should be greater than 0.

Formulas to find Column Efficiency

Retention Volume

The Retention Volume is defined as the volume of the mobile phase required to elute the solute from the column.

Symbol: V_R

Measurement: VolumeUnit: L

Note: Value can be positive or negative.

Formulas to find Retention Volume

Width of Chromatographic Peak

Width of Chromatographic Peak is the distance between points where lines tangent to the peak's left and right inflection points intersect the baseline.

Symbol: W_b

Measurement: LengthUnit: mm

Note: Value should be greater than 0.

Formulas to find Width of Chromatographic Peak

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How to Evaluate Column Efficiency in Chromatography?

Column Efficiency in Chromatography evaluator uses Column Efficiency = 16*((Retention Volume/Width of Chromatographic Peak)^2) to evaluate the Column Efficiency, The Column Efficiency in Chromatography formula is defined as a measure of the dispersion of a peak. Narrow peaks take up less space in the chromatogram and thus allow more peaks to be separated. Column Efficiency is denoted by N symbol.

How to evaluate Column Efficiency in Chromatography using this online evaluator? To use this online evaluator for Column Efficiency in Chromatography, enter Retention Volume (V_R) & Width of Chromatographic Peak (W_b) and hit the calculate button.

FAQs on Column Efficiency in Chromatography

What is the formula to find Column Efficiency in Chromatography?

The formula of Column Efficiency in Chromatography is expressed as Column Efficiency = 16*((Retention Volume/Width of Chromatographic Peak)^2). Here is an example- 3.211264 = 16*((0.0112/0.025)^2).

How to calculate Column Efficiency in Chromatography?

With Retention Volume (V_R) & Width of Chromatographic Peak (W_b) we can find Column Efficiency in Chromatography using the formula - Column Efficiency = 16*((Retention Volume/Width of Chromatographic Peak)^2).

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