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Method of Separation Technique
Retention Volume in Method of Separation Technique Formulas
The Retention Volume is defined as the volume of the mobile phase required to elute the solute from the column. And is denoted by V
R
. Retention Volume is usually measured using the Liter for Volume. Note that the value of Retention Volume is always negative.
Method of Separation Technique formulas that make use of Retention Volume
f
x
Retention Time given Retention Volume
Go
f
x
Flow Rate given Retention Volume and Time
Go
f
x
Unretained Volume given Capacity Factor
Go
f
x
Capacity Factor given Retention Volume and Unretained Volume
Go
FAQ
What is the Retention Volume?
The Retention Volume is defined as the volume of the mobile phase required to elute the solute from the column. Retention Volume is usually measured using the Liter for Volume. Note that the value of Retention Volume is always negative.
Can the Retention Volume be negative?
Yes, the Retention Volume, measured in Volume can be negative.
What unit is used to measure Retention Volume?
Retention Volume is usually measured using the Liter[L] for Volume. Cubic Meter[L], Cubic Centimeter[L], Cubic Millimeter[L] are the few other units in which Retention Volume can be measured.
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